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1.
Field observations of two sympatric pitheciine species reveal that the positional repertoire of the white-faced saki, Pithecia pithecia, is dominated by leaping behaviors, whereas the bearded saki, Chiropotes satanas, is predominantly quadrupedal. Examination and comparison of the postcranial skeletal morphologies and limb proportions of these species display numerous features associated with their respective locomotor behaviors. These observations accord with associations found in other primate and mammalian groups and with predictions based on theoretical and experimental biomechanics. Preliminary observations of the skeletal morphology of Cacajao calvus demonstrate a marked similarity to that of Chiropotes. The fossil platyrrhine Cebupithecia sarmientoi displays greater similarity to Pithecia, suggesting that its positional repertoire also included significant leaping and clinging behaviors. 相似文献
2.
Muscle fine structure reflects ecotype in two nototheniids 总被引:3,自引:0,他引:3
The fine structure of swimming (pectoral) and myotomal (axial) skeletal muscle and myocardium of two species of Antarctic nototheniid fishes were studied by electron microscopy, comparing the cryopelagic Pagothenia borchgrevinki and the benthic Trematomus bernacchii . Mean fibre size varied by a factor of four among muscles within each species and may have reflected the locomotory power available, being larger in pectoral oxidative (red) and axial glycolytic (white) muscle of P. borchgrevinki . Both species use labriform locomotion, and the more active P. borchgrevinki had a greater capillary supply, expressed as a capillary to fibre ratio, than T. bernacchii to both red (3·48 ± 0·36 v . 1·63 ± 0·14, mean ± s . e .; P < 0·01) and white (2·70 ± 0·20 v . 1·53 ± 0·18, mean ± s . e .; P < 0·01) regions of the pectoral musculature. The greater aerobic scope of P. borchgrevinki was strikingly demonstrated in the higher mitochondrial content of all skeletal muscle types sampled, and the ventricular myocardium (0·269 ± 0·011 v . 0·255 ± 0·012 mean ± s . e .; P < 0·05). Minor differences were found in other elements of fibre composition, with the exception of a five‐fold greater lipid content in pectoral red fibres of P. borchgrevinki (0·074 ± 0·014 mean ± s . e .) v . T. bernacchii (0·010 ± 0·003; P < 0·05). Differences in muscle fine structure among species clearly reflected differences in their ecotype. 相似文献
3.
The uptake of two different preparations of99mTechnetium-methylene diphosphonate in fetal rat calvaria is compared. The localization of99mTc after administration of99mTc(Sn)-MDP and99mTc-MDP showed equal distribution in autoradiography. 相似文献
4.
E. Golomer P. Dupui H. Monod 《European journal of applied physiology and occupational physiology》1997,76(2):140-144
We investigated the effects of maturation on the dynamic body sways of healthy girls. Prepubertal and postpubertal girls
practising professional physical activities requiring a good ability to maintain equilibrium (acrobats and dancers) were asked
to stand on a free seesaw platform and the results compared to those for untrained age-matched girls. This platform (stabilometer)
allows self-induced body sways. Stabilograms were obtained by a double integration of the angular acceleration from the recordings
of the platform sways made with an accelerometer. Fast Fourier transform processing of stabilograms allowed spectral frequency
analysis. The total spectrum energy and the energies of three frequency bands (0–0.5 Hz, 0.5–2 Hz, 2–20 Hz) were determined.
ANOVA showed that, for all groups of different equilibrium activity and independent of visual input, prepubertal girls had
higher energy values than postpubertal girls in the 0- to 0.5-Hz band whereas the opposite was true for 0.5- to 2-Hz band.
Ballet dancers were more dependent than acrobats on visual inputs for the regulation of their postural control but were less
dependent than untrained girls at both ages. Maturation seemed to shift body sways towards higher frequencies and the utilization
of the cues of postural control was different according to the type of equilibrium activity practised by the subjects.
Accepted: 7 February 1997 相似文献
5.
Y. Saijo S. Takeda A. Scherer T. Kobayashi Y. Mada H. Taniguchi M. Yao S. Wakatsuki 《Protein science : a publication of the Protein Society》1997,6(4):916-918
Troponin is a ternary protein complex consisting of subunits TnC. TnI, and TnT, and plays a key role in calcium regulation of the skeletal and cardiac muscle contraction. In the present study, a partial complex (CI47) was prepared from Escherichia coli-expressed rabbit skeletal muscle TnC and fragment 1-47 of TnI, which is obtained by chemical cleavage of an E. coli-expressed mutant of rabbit skeletal muscle TnI. Within the ternary troponin complex, CI47 is thought to form a core that is resistant to proteolytic digestion, and the interaction within CI47 likely maintains the integrity of the troponin complex. Complex CI47 was crystallized in the presence of sodium citrate. The addition of trehalose improved the diffraction pattern of the crystals substantially. The crystal lattice belongs to the space group P3(1)(2)21, with unit cell dimensions a = b = 48.2 A, c = 162 A. The asymmetric unit presumably contains one CI47 complex. Soaking with p-chloromercuribenzenesulfonate (PCMBS) resulted in loss of isomorphism, but enhanced the quality of the crystals. The crystals diffracted up to 2.3 A resolution, with completeness of 91% and R(merge) = 6.4%. The crystals of PCMBS-derivative should be suitable for X-ray studies using the multiple-wavelength anomalous diffraction technique. This is the first step for elucidating the structure of the full troponin complex. 相似文献
6.
A computerized mechanical cell stimulator for tissue culture: Effects on skeletal muscle organogenesis 总被引:8,自引:0,他引:8
Herman H. Vandenburgh 《In vitro cellular & developmental biology. Plant》1988,24(7):609-619
Summary A tissue culture system has been developed which can mechanically stimulate cells growing on a highly elastic plastic substratum
in a 24-well cell growth chamber. The collagen-coated substratum to which the cells attach and grow in the Mechanical Cell
Stimulator (Model I) can be repetitively stretched and relaxed by stepper motor with linear accuracy of 30 μm. The activity
controlling unit is an Apple IIe computer interfaced with the cell growth chamber via optical data links and is capable of
simulating many of the mechanical activity patterns that cells are subjected to in vivo. Primary avian skeletal myoblasts
proliferate and fuse into multinucleated myotubes in this set-up in a manner similar to normal tissue culture dishes. Under
static culture conditions, the muscle cells differentiate into networks of myotubes which show little orientation. Growing
the proliferating muscle cells on a unidirectional stretching substratum causes the developing myotubes to orient parallel
to the direction of movement. In contrast, growing the cells on a substratum undergoing continuous stretch-relaxation cycling
orients the developing myotubes perpendicular to the direction of movement. Neither type of mechanical activity significantly
affects the rate of cell proliferation of the rate of myoblast fusion into myotubes. These results indicate that during in
vivo skeletal muscle organogenesis, when substantial mechanical stresses are placed on skeletal muscle cells by both continuous
bone elongation and by spontaneous contractions, only bone elongation plays a significant role in proper fiber orientation
for subsequent functional work.
Supported by grants NS16753, AR36266, and RR05818 from the National Institutes of Health, Bethesda, MD. 相似文献
7.
Alan D. Fleming Thomas J. Kuehl David T. Armstrong 《Molecular reproduction and development》1985,11(2):107-119
Pig oocytes obtained from slaughterhouse material and rat oocytes obtained from PMSG-treated immature females were incubated as isolated oocytes or injected into explanted pig follicles (5–8 mm). Free oocytes of both species, with or without their cumulus investment or gonadotropins during culture, matured at high rates after 30 hr or 9–10 hr of culture, respectively. Gonadotropic stimulation was necessary for maturation of both the native and injected cumulus-intact pig oocytes in follicle culture. Cumulus-free pig oocytes injected into follicle failed to mature in response to gonadotropic stimulation, suggesting an inability to perceive or respond to stimulation. Injected rat oocytes, however, matured irrespective of cumulus investment or gonadotropic stimulation. Their maturation was delayed and reduced at 9 hr. These results in the rat suggest that the pig follicular environment is incapable of regulating rat oocyte maturation but rather presents a permissive or supportive environment for their maturation. The explanted surrogate follicles from the pig or other species may provide a useful model for the study of oocyte-follicle interactions in oocyte maturation within or between species. 相似文献
8.
Protein synthesis and secretion in the human epididymis and immunoreactivity with sperm antibodies 总被引:1,自引:0,他引:1
P Ross F W Kan P Antaki N Vigneault A Chapdelaine K D Roberts 《Molecular reproduction and development》1990,26(1):12-23
The synthesis and secretion of proteins in the different regions of the human epididymis were studied in vitro. Epididymal tissues obtained from patients undergoing castration for prostatic carcinoma or from cadavers were incubated in the presence of [35S]methionine, and the resulting radiolabeled proteins were analysed on SDS-PAGE. The corpus region was found to be the most active segment in total protein synthesis. Significant qualitative and quantitative changes were observed in the pattern of proteins secreted from the different epididymal regions. To establish those epididymal proteins that interact with maturing sperm, the secreted products were immunoreacted with antibodies raised against a Triton X-100 extract of ejaculated human sperm heads. The antibodies react mainly with the head region of ejaculated spermatozoa as judged by indirect immunofluorescence. Protein A-gold labeling of freeze-fracture images showed gold particle distribution on the sperm plasma membrane. Western blot analysis of the secreted proteins revealed four bands (66, 37, 32, and 29 kDa) in the proximal regions and six additional bands (80, 76, 48, 27, 22, and 17 kDa) in the distal part of the epididymis. Immunoprecipitation of the secreted proteins with these antibodies revealed six radioactive bands of 170, 80, 76, 60, 48, and 37 kDa, which indicates that certain proteins of epididymal origin bind to the sperm plasma membrane. 相似文献
9.
10.