全文获取类型
收费全文 | 2466篇 |
免费 | 137篇 |
国内免费 | 483篇 |
专业分类
3086篇 |
出版年
2023年 | 43篇 |
2022年 | 37篇 |
2021年 | 64篇 |
2020年 | 73篇 |
2019年 | 80篇 |
2018年 | 65篇 |
2017年 | 66篇 |
2016年 | 80篇 |
2015年 | 105篇 |
2014年 | 101篇 |
2013年 | 154篇 |
2012年 | 93篇 |
2011年 | 86篇 |
2010年 | 89篇 |
2009年 | 88篇 |
2008年 | 98篇 |
2007年 | 101篇 |
2006年 | 111篇 |
2005年 | 132篇 |
2004年 | 92篇 |
2003年 | 99篇 |
2002年 | 102篇 |
2001年 | 75篇 |
2000年 | 89篇 |
1999年 | 71篇 |
1998年 | 78篇 |
1997年 | 67篇 |
1996年 | 70篇 |
1995年 | 52篇 |
1994年 | 60篇 |
1993年 | 72篇 |
1992年 | 64篇 |
1991年 | 63篇 |
1990年 | 52篇 |
1989年 | 42篇 |
1988年 | 49篇 |
1987年 | 21篇 |
1986年 | 15篇 |
1985年 | 34篇 |
1984年 | 20篇 |
1983年 | 11篇 |
1982年 | 19篇 |
1981年 | 17篇 |
1980年 | 17篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1977年 | 8篇 |
1975年 | 12篇 |
1974年 | 12篇 |
1973年 | 8篇 |
排序方式: 共有3086条查询结果,搜索用时 0 毫秒
1.
A M Belles T J Montville B P Wasserman 《Journal of industrial microbiology & biotechnology》2000,24(1):71-74
Alcohol-extractable, hydrophobic zein proteins contaminate starch granule surfaces and can be removed by enzymatic digestion
with thermolysin. The goal of this research was to find practical alternatives to thermolysin that might be used during the
corn wet-milling process. All of the commercial thermostable alkaline proteases studied (SP 709, Neutrase, and Spezyme FAN)
removed the zein proteins from various types of cornstarch, as demonstrated by the lack of protein bands below 30 kDa under
the reducing conditions of SDS-PAGE gel. Each enzyme removed the zein proteins as effectively as thermolysin removed them.
However, the removal of the zein protein did not reduce the quantity of free fatty acids associated with the starch. Journal of Industrial Microbiology & Biotechnology (2000) 24, 71–74.
Received 27 May 1999/ Accepted in revised form 01 October 1999 相似文献
2.
3.
Production of trichothecene and non-trichothecene mycotoxins by Fusarium species isolated from maize in Minnesota 总被引:1,自引:0,他引:1
Eighty-two cultures of Fusarium species isolated in 1986 from moldy maize in Minnesota were each cultured on rice for 4 weeks and found to produce the following mycotoxins: F. graminearum isolates, deoxynivalenol (DON, 4–225 g/g), 3-acetyldeoxynivalenol (3-ADON, 2–4g/g), 15-acetyldeoxynivalenol (15-ADON, 1–35 g/g) and zearalenone (ZEA, 5–4350 g/g); F. moniliforme, fusarin C (detectable amounts to 1000 g/g); F. mòniliforme, F. oxysporum, F. proliferatum and F. subglutinans isolates, moniliformin (15–6775 g/g); F. moniliforme, F. proliferatum, and F. subglutinans isolates, fusaric acid (detectable amounts). Other mycotoxins screened for in each rice sample and not detected were T-2 toxin, HT-2 toxin, neosolaniol, T-2 tetraol, nivalenol, fusarenon-X, scirpenols, alpha and beta trans-zearalenols, wortmannin, and fusarochromanone. The rat feeding bioassay indicated that other, unidentified toxins may be present. 相似文献
4.
Tim Helentjaris Gretchen King Mary Slocum Chris Siedenstrang Sharon Wegman 《Plant molecular biology》1985,5(2):109-118
Summary Maize and tomato cDNA clones have been hybridized in Southern blotting experiments to plant genomic DNA prepared from different lines to detect restriction fragment polymorphisms (RFPs). In maize we have found that a high degree of genetic variability is present, even among domestic inbred lines. Most randomly chosen maize cDNA clones can be used to detect elements of this variability. Similar levels of polymorphism are observed when genomic DNA is digested with any of a number of different restriction enzymes and probed with individual clones. When a clone is hybridized to genomic DNAs prepared from several different maize lines, a number of different alleles are often detected at a single locus. At the same time one clone can often detect more than one independently segregating locus by cross hybridization to related sequences at other loci. As expected these markers are inherited as simple codominant Mendelian alleles from one generation to the next and colinkage of these markers can be demonstrated in the progeny from a heterozygous parent. In similar studies with tomato, remarkably different results were found. Few RFPs were demonstrable among domestic Lycopersicon esculentum lines although a higher level of variability could be detected when comparing esculentum with its wild Lycopersicon relatives. These results are discussed in relation to the applied uses of RFPs in plant breeding as well as the inherent variability of different plant genomes.This work was supported in part by funds from Sandoz Ltd. (Basel, Switzerland) and its subsidiary company, Northrup King Co. (Minneapolis, Minn., U.S.A.) as well as by NSF SBIR grant #BSR-8360870. 相似文献
5.
Roots of ten-days-old seedlings obtained from a maize hybrid grown in complete or in sulphate-deprived medium were used to extract Poly(A)+RNA. The response to sulphate deprivation, which is known to increase the uptake capacity up to ten times, was manifested also by the expression of three mRNA species, as shown by the in vitro translation of the mRNA population. One hour after transfer to complete nutrient medium all three mRNAs were still present.Abbreviations BSA
Bovine Serum Albumine
- DTT
1,4-Dithio-DL-Threitol
- EDTA
Ethylenediaminetetraacetic-acid
- MES
2-(N-Morpholino) ethane sulfonic acid
- SDS
Sodium Dodecyl Sulfate
- TCA
Trichloracetic acid
- TRIS
Tris(hydroxy-methyl)-aminomethane 相似文献
6.
Influence of mechanical impedance on root exudation of maize seedlings at two development stages 总被引:4,自引:0,他引:4
Studies were undertaken to evaluate the effects of mechanical impedance on root exudation by maize (Zea mays L., var Dea) and to examine the importance of these effects in relation to the stage of plant development. Plants were grown under sterile and hydroponic conditions. Mechanical impedance was simulated using glass beads of 1 mm diameter. This treatment was compared with a control without beads. Results demonstrated that plant growth was influenced by mechanical impedance. Mechanical impedance markedly affected the growth of the shoot, whether this was measured as leaf area or total dry matter. Besides increasing root/shoot biomass ratios, mechanical impedances also stimulated root exudation of organic and inorganic compounds. Stressed plants lost more nitrogenous compounds than control plants. Otherwise, the percentage of released carbon decreased. Depending on the developmental stage of the plant, there was a large variation in the magnitude and time course on mechanical impedance effects. The effects of mechanical impedance persist and accentuate with time. 相似文献
7.
Dynamics of soil microbial biomass N under zero and shallow tillage for spring wheat,using15N urea 总被引:2,自引:0,他引:2
Summary Field studies to determine the effect of zero and shallow (10 cm) cultivation on microbial biomass were conducted on several Chernozemic soils in western Canada. Using the CHCl3 fumigation method, the distribution of microbial biomass N and the immobilization and subsequent release of added15N (15N-urea) from the microbial biomass were determined in the A horizon, at the 0 to 5 and 5 to 10 cm depth, during the growing season for spring wheat.Temporal variation in microbial biomass N, associated with the development of the rhizosphere, was characterized by an increase between Feekes stage 1 and 5 or 10 and decrease at Feekes stage 11.4. Over the long term, the variation in biomass N between tillage systems corresponded with crop residue distribution. Immobilization of fertilizer N was related to the increase in biomass N from Feekes stage 1, which in turn, was associated with the incorporation of recent crop residues or levels of labile organic matter in the surface soil. The study demonstrated the relatively rapid remineralization of immobilized fertilizer N under field conditions and emphasized the role of the microbial biomass N as both a sink and source of mineral N. 相似文献
8.
Abstract Effect of ammonium on in vivo activity of nitrate reductase in roots, shoots and leaves of maize (Zea mays L.) seedlings was studied in relation to light/dark conditions and EDTA supply. Supply of 5 mM (NH4)2SO4 increased the steady state level of enzyme only in leaves and in light, while it had no effect in roots and shoots and in the dark. The substrate induction of enzyme was also little affected by 1 to 10 mM (NH4)2SO4 in roots and shoots. In the leaves the activity in the dark was either inhibited (minus EDTA) or stimulated (plus EDTA) by 5 to 10 mM (NH4)2SO4. The activity was stimulated in the light also in the presence of EDTA at higher concentrations of ammonium. When different concentrations of ammonium were supplied without any exogenous nitrate in the light, the enzyme activity increased at low concentration and was either inhibited or unaffected at higher concentrations depending upon the tissue used. Supply of EDTA with ammonium modified its effect to some extent. It is suggested that the effect of ammonium on nitrate reductase activity depends upon the tissue used and the effective concentration of the ammonium. 相似文献
9.
1961-2013年东北三省玉米低温冷害强度的时空分布特征 总被引:4,自引:0,他引:4
基于东北三省1961-2013年气象数据、《北方春玉米冷害评估技术规范(QX/T167-2012)行业标准》,结合具有生物学意义的热量指数,给出了东北三省低温冷害强度分级,利用经验正交函数(EOF)分解法和旋转经验正交函数(REOF)分解法分析了东北三省玉米低温冷害强度时空分布特征。结果表明:东北三省玉米低温冷害强度具有明显的空间分布特点,可分为全区一致型、南北反相位分布型以及东西反相位分布型。东北三省玉米低温冷害强度的时间变化具有较好的一致性,1961-2013年东北三省低温冷害的发生强度呈波动减小趋势。低温冷害强度区域差异显著,按照低温冷害强度强弱可分为黑龙江-吉林北部区(Ⅰ区)、吉林南部-辽宁北部区(Ⅲ区)和辽宁南部区(Ⅱ区)。研究结果可为东北三省低温冷害风险区划和采取有效的减灾避灾措施提供依据。 相似文献
10.
玉米高直链淀粉育种是玉米分子育种的一个重要研究方向.本实验中,首先研究了不同诱导愈伤培养基对再生体系的影响,确定了LS+2,4-D 2.0 mg/L+L-pro 700 mg/L+CH 500 mg/L+3 %蔗糖为诱导培养基.同时,构建并验证了含有淀粉分支酶sbeIIb基因双干涉片段载体和胚乳特异性启动子的表达载体pCAMBIA 1301+Glu+1620,并转入根癌农杆菌EHA105,以农杆菌转化法转化玉米自交系178.通过PCR检测,5株转化株表现阳性,初步证明了干涉片段已整合入玉米基因组中. 相似文献