排序方式: 共有8条查询结果,搜索用时 0 毫秒
1
1.
2.
Sequence-specific DNA recognition by polyamides 总被引:12,自引:0,他引:12
3.
A Pseudomonas fluorescens was found to degrade and utilize a polyester polyurethane as a sole carbon and energy source. Polyurethane utilization by P. fluorescens. followed simple Michaelis–Menten kinetics. The Ks and μmax values were 0.9 mg ml−1 and 1.61 doublings · h−1, respectively. The enzymes from P. fluorescens responsible for polyurethane degradation were found to be extracellular. Analysis of the polyurethane degrading proteins using non-denaturing polyacrylamide gel electrophoresis revealed one active protein band with an Rf value of 0.083. A polyurethane degrading enzyme was purified and displayed protease activity. This enzyme was inhibited by phenylmethylsulfonyl fluoride and had a molecular weight of 29,000 daltons. 相似文献
4.
Le NM Sielaff AM Cooper AJ Mackay H Brown T Kotecha M O'Hare C Hochhauser D Lee M Hartley JA 《Bioorganic & medicinal chemistry letters》2006,16(24):6161-6164
An N-formamido pyrrole- and imidazole-containing triamide (f-PIP) has been shown by DNase I footprinting, SPR, and CD studies to bind as a stacked dimer to its cognate sequences: 5'-TACGAT-3' (5'-flank of the inverted CCAAT box-2 of the human topoisomerase IIalpha promoter) and 5'-ATCGAT-3'. A gel shift experiment provided evidence for f-PIP to inhibit protein-DNA interaction at the ICB2 site. Western blot studies showed that expression of the topoisomerase IIalpha gene in confluent NIH 3T3 cells was induced by treatment with f-PIP. The results suggested that the triamide was able to enter the nucleus, interacted with the target site within ICB2, inhibited NF-Y binding, and activated gene expression. 相似文献
5.
Sameer Chavda Balaji Babu Pravin Patil Adam Plaunt Amanda Ferguson Megan Lee Samuel Tzou Robert Sjoholm Toni Rice Hilary Mackay Joseph Ramos Shuo Wang Shicai Lin Konstantinos Kiakos W. David Wilson John A. Hartley Moses Lee 《Bioorganic & medicinal chemistry》2013,21(13):3907-3918
Orthogonally positioned diamino/dicationic polyamides (PAs) have good water solubility and enhanced binding affinity, whilst retaining DNA minor groove and sequence specificity compared to their monoamino/monocationic counterparts. The synthesis and DNA binding properties of the following diamino PAs: f-IPI (3a), f-IPP (4), f-PIP (5), and f-PPP (6) are described. P denotes the site where a 1-propylamino group is attached to the N1-position of the heterocycle. Binding of the diamino PAs to DNA was assessed by DNase I footprinting, thermal denaturation, circular dichroism titration, biosensor surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC) studies. According to SPR studies, f-IPI (3a) bound more strongly (Keq = 2.4 × 108 M?1) and with comparable sequence selectivity to its cognate sequence 5′-ACGCGT-3′ when compared to its monoamino analog f-IPI (1). The binding of f-IPI (3a) to 5′-ACGCGT-3′ via the stacked dimer motif was balanced between enthalpy and entropy, and that was quite different from the enthalpy-driven binding of its monoamino parent f-IPI (1). f-IPP (4) also bound more strongly to its cognate sequence 5′-ATGCAT-3′ (Keq = 7.4 × 106 M?1) via the side-by-side stacked motif than its monoamino analog f-IPP (2a). Although f-PPP (6) bound via a 1:1 motif, it bound strongly to its cognate sequence 5′-AAATTT-3′ (Keq = 4.8 × 107 M?1), 15-times higher than the binding of its monoamino analog f-PPP (2c), albeit f-PPP bound via the stacked motif. Finally, f-PIP (5) bound to its target sequence 5′-ATCGAT-3′ as a stacked dimer and it has the lowest affinity among the diamino PAs tested (Keq <1 × 105 M?1). This was about two times lower in affinity than the binding of its monoamino analog f-PIP (2b). The results further demonstrated that the ‘core rules’ of DNA recognition by monoamino PAs also apply to their diamino analogs. Specifically, PAs that contain a stacked IP core structure bind most strongly (highest binding constants) to their cognate GC doublet, followed by the binding of PAs with a stacked PP structure to two degenerate AT base pairs, and finally the binding of PAs with a PI core to their cognate CG doublet. 相似文献
6.
Babu B Liu Y Plaunt A Riddering C Ogilvie R Westrate L Davis R Ferguson A Mackay H Rice T Chavda S Wilson D Lin S Kiakos K Hartley JA Lee M 《Biochemical and biophysical research communications》2011,(3):848-852
An orthogonally positioned diamino/dicationic polyamide f-IPI 2 was synthesized. It has enhanced binding affinity, and it showed comparable sequence specificity to its monoamino/monocationic counterpart f-IPI 1. Results from CD and DNase I footprinting studies confirmed the minor groove binding and selectivity of polyamides 1 and 2 for the cognate sequence 5′-ACGCGT-3′. SPR studies provided their binding constants: 2.4 × 108 M−1 for diamino 2, which is ∼4 times higher than 5.4 × 107 M−1 for its monoamino analogue 1. 相似文献
7.
8.
Naoki Shiga Shihori Takayanagi Risa Muramoto Tasuku Murakami Rui Qin Yuta Suzuki Ken-ichi Shinohara Atsushi Kaneda Tetsuhiro Nemoto 《Bioorganic & medicinal chemistry letters》2017,27(10):2197-2200
Pyrrole-imidazole (Py-Im) polyamides are useful tools for chemical biology and medicinal chemistry studies due to their unique binding properties to the minor groove of DNA. We developed a novel method of synthesizing Py-Im polyamide oligomers based on a Cu-catalyzed cross-coupling strategy. All four patterns of dimer fragments could be synthesized using a Cu-catalyzed Ullmann-type cross-coupling with easily prepared monomer units. Moreover, we demonstrated that pyrrole dimer, trimer, and tetramer building blocks for Py-Im polyamide synthesis were accessible by combining site selective iodination of the pyrrole/pyrrole coupling adduct. 相似文献
1