全文获取类型
收费全文 | 1720篇 |
免费 | 110篇 |
国内免费 | 52篇 |
专业分类
1882篇 |
出版年
2024年 | 5篇 |
2023年 | 20篇 |
2022年 | 22篇 |
2021年 | 41篇 |
2020年 | 29篇 |
2019年 | 49篇 |
2018年 | 40篇 |
2017年 | 46篇 |
2016年 | 47篇 |
2015年 | 49篇 |
2014年 | 104篇 |
2013年 | 86篇 |
2012年 | 62篇 |
2011年 | 94篇 |
2010年 | 63篇 |
2009年 | 104篇 |
2008年 | 119篇 |
2007年 | 122篇 |
2006年 | 81篇 |
2005年 | 58篇 |
2004年 | 62篇 |
2003年 | 49篇 |
2002年 | 28篇 |
2001年 | 30篇 |
2000年 | 37篇 |
1999年 | 29篇 |
1998年 | 29篇 |
1997年 | 37篇 |
1996年 | 22篇 |
1995年 | 20篇 |
1994年 | 26篇 |
1993年 | 24篇 |
1992年 | 13篇 |
1991年 | 19篇 |
1990年 | 14篇 |
1989年 | 16篇 |
1988年 | 18篇 |
1987年 | 19篇 |
1986年 | 15篇 |
1985年 | 13篇 |
1984年 | 12篇 |
1983年 | 8篇 |
1982年 | 19篇 |
1981年 | 11篇 |
1980年 | 16篇 |
1979年 | 8篇 |
1978年 | 6篇 |
1977年 | 13篇 |
1973年 | 5篇 |
1972年 | 5篇 |
排序方式: 共有1882条查询结果,搜索用时 8 毫秒
1.
R. C. Griffiths S. W. McKechnie J. A. McKenzie 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1982,62(1):89-96
Summary Mother-offspring data for alcohol dehydrogenase genotypes of a vineyard cellar population of D. melanogaster are best explained by a model that allows 21% of females in the population to mate twice with an 83% level of sperm displacement. A population model with multiple mating and sperm displacement is examined theoretically. A formula for the effective population size is derived under this model. Multiple mating increases the effective population size relative to single mating. 相似文献
2.
This scanning-electron microscopic study demonstrates the convergent evolution of the mouthparts of various herbivorous stream animals (insects from different orders, an isopod, snails, fish, and a tadpole) feeding on epilithic algal pastures. This food source is rich but is often difficult to harvest. Nevertheless, a large number of species can live on it because they have evolved highly specialized mouthparts. There are four functional problems that an algae grazer has to solve: the algae must be removed from the stone, they have to be collected and crushed, and a current shield is needed to prevent the water flow sweeping away the food. Among the 30 algae grazers examined in this study, a limited number of morphological solutions have been found for each of these adaptational problems. There are multiple evolutionary pathways for mouthpart adaptation and even closely related species have often evolved different types of tools for the same function. This refects the existence of a certain amount of evolutionary scope. Such freedom of evolution is present, however, only at the beginning of the adaptiogenesis of an algae grazer. Once one of the evolutionary pathways is taken, further improvement of the mouthparts is possible only by the refinement of the ‘chosen’ type of tools. The consequence of this is that a large number of astonishing convergences have occurred in algae grazers that have independently trodden the same evolutionary pathway. 相似文献
3.
P. Angell W.J. Machowski P.P. Paul C.M. Wall F.F. Lyle Jr. 《Journal of microbiological methods》1997,30(3):173-178
A multiple chemostat system has been developed in which metal specimens can be exposed to a consortium of bacteria. The system comprises a single test chemostat containing the test specimen operated at a high dilution rate to facilitate the wash out of planktonic bacteria, selecting for attached or biofilm growth. This chemostat is fed at a steady low rate by a number of separate chemostats each of which contains a pure axenic culture of one member of the consortium being tested. This system has the advantage of providing a continual inoculum of the test species to the test specimen allowing both aerobic and anaerobic bacteria to be grown in the same system. Constant levels of three bacterial types were maintained in the system: Pseudomonas aeruginosa, Thiobacillus ferrooxidans and Desulfovibrio vulgaris. Exposure of 316L stainless steel electrodes to this system resulted in increased corrosion of coupons exposed biotically, as compared to those exposed abiotically. A current monitoring technique and electrochemical impedance spectroscopy were used to evaluate effects of bacteria on metallic corrosion. 相似文献
4.
Kristel Van Steen Nadia Tahri Geert Molenberghs 《Biometrical journal. Biometrische Zeitschrift》2004,46(2):187-202
Until recently, the most common parametric approaches to study the combined effects of several genetic polymorphisms located within a gene or in a small genomic region are, at the genotype level, logistic regressions and at the haplotype level, haplotype analyses. An alternative modeling approach, based on the case/control principle, is to regard exposures (e.g., genetic data such as derived from Single Nucleotide Polymorphisms – SNPs) as random and disease status as fixed and to use a marginal multivariate model that accounts for inter‐relationships between exposures. One such model is the multivariate Dale model. This model is based on multiple logistic regressions. That is why the model, applied in a case/control setting, leads to straightforward interpretations that are similar to those drawn in a classical logistic modeling framework. (© 2004 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim) 相似文献
5.
A flexible method to align large numbers of biological sequences 总被引:5,自引:0,他引:5
William R. Taylor 《Journal of molecular evolution》1988,28(1-2):161-169
Summary A method for the alignment of two or more biological sequences is described. The method is a direct extension of the method of Taylor (1987) incorporating a consensus sequence approach and allows considerable freedom in the control of the clustering of the sequences. At one extreme this is equivalent to the earlier method (Taylor 1987), whereas at the other, the clustering approaches the binary method of Feng and Doolittle (1987). Such freedom allows the program to be adapted to particular problems, which has the important advantage of resulting in considerable savings in computer time, allowing very large problems to be tackled. Besides a detailed analysis of the alignment of the cytochrome c superfamily, the clustering and alignment of the PIR sequence data bank (3500 sequences approx.) is described. 相似文献
6.
7.
Synopsis Aspects of the life history of Barbus anoplus were studied in Lake le Roux, a turbid man-made lake on the Orange River, South Africa. This minnow underwent a population
explosion and successfully colonized the shoreline of the newly-formed lake during the early phases of reservoir filling.
Male and female B. anoplus reach sexual maturity in one year at about 40 mm fork length. They have a multiple spawning habit with the first spawning
in November–January and the second in February–March. The growth of the two resulting cohorts is discussed. It is proposed
that the offspring from the second spawning not only acts as a ‘back-up’ but is capable of prolonging the life of that year-class
into an additional reproductive season. Most of the minnows die after their second summer, but more offspring from the second
spawning, especially females, live into a third summer. Females attain a larger maximum size (73 mm FL) and age (3–4 years)
than males (60 mm FL, 2–3 years). B. anoplus is small and short-lived with a high seasonal reproductive potential, which is in contrast to the larger Barbus species in the Orange River system. These life-history traits enable the species to colonize and successfully inhabit unstable
environments and probably account for its widespread distribution. 相似文献
8.
A multivariate t probability integral 总被引:2,自引:0,他引:2
9.
Plots of P-values to evaluate many tests simultaneously 总被引:3,自引:0,他引:3
10.
Differing Neurochemical and Morphological Sequelae of Global Ischemia: Comparison of Single- and Multiple-Insult Paradigms 总被引:3,自引:0,他引:3
Baowan Lin Mordecai Y.-T. Globus W. Dalton Dietrich Raul Busto Elena Martinez Myron D. Ginsberg 《Journal of neurochemistry》1992,59(6):2213-2223
The purpose of this investigation was to investigate pathomechanisms responsible for the deleterious effects of repeated episodes of brief forebrain ischemia. Halothane-anesthetized male Wistar rats were subjected to either (a) a single 15-min period or (b) three 5-min periods (separated by 1 h) of global forebrain ischemia by bilateral carotid artery occlusions plus hypotension (50 mm Hg), followed by various periods of recirculation. Brain temperature was normothermic throughout. In one series of rats, extracellular levels of glutamate, glycine, and gamma-aminobutyric acid (GABA) were measured in the dorsolateral striatum (n = 6-8 per group) and lateral thalamus (n = 4-6 per group) by microdialysis and HPLC before and during ischemia and during 3-5 h of recirculation. In a parallel series of rats (n = 6 per group), ischemic cell change was quantified at 2 (dark neurons), 24, or 72 h following either single or multiple ischemic insults. A single 15-min ischemic period led to massive glutamate release (13-fold increase; p = 0.001), which returned to normal by 20-30 min of recirculation and remained normal thereafter. By contrast, in rats with three 5-min periods of ischemia, the glutamate level rise with each repeated insult (four- to 4.5-fold; p < or = 0.02) was smaller than that observed during the single 15-min insult, but a late sustained rise (five- to six-fold; p < 0.05) occurred at 2-3 h of recirculation. Brief ischemia-induced elevations of glycine and GABA levels were detected in both the single- and multiple-insult groups, with normalization during recirculation. In contrast, the excitotoxic index, a composite measure of neurotransmitter release ([glutamate] x [glycine]/[GABA]), differed markedly following single versus multiple insults (p = 0.002 by repeated-measures analysis of variance) and increased by seven- to 12-fold (p < 0.05) at 1-3 h following the third insult. The total amount of glutamate released was 3.3-fold higher in the multiple-insult than in the single-insult group (p < 0.02). At 2 h of recirculation, histopathological analysis of dorsolateral striatum showed a significantly greater frequency of dark neurons in the multiple- than in the single-insult group (p < 0.05 by analysis of variance). In the thalamus, a higher frequency of ischemic neurons was seen in the multiple-than in the single-insult group at all intervals studied. Thus, in rats with multiple ischemic insults, accelerated ischemic damage was found in the striatum, and severe ischemic injury was documented in the thalamus.(ABSTRACT TRUNCATED AT 400 WORDS) 相似文献