全文获取类型
收费全文 | 1903篇 |
免费 | 29篇 |
国内免费 | 36篇 |
专业分类
1968篇 |
出版年
2023年 | 11篇 |
2022年 | 23篇 |
2021年 | 8篇 |
2020年 | 24篇 |
2019年 | 17篇 |
2018年 | 18篇 |
2017年 | 43篇 |
2016年 | 64篇 |
2015年 | 32篇 |
2014年 | 57篇 |
2013年 | 100篇 |
2012年 | 45篇 |
2011年 | 80篇 |
2010年 | 59篇 |
2009年 | 103篇 |
2008年 | 140篇 |
2007年 | 123篇 |
2006年 | 96篇 |
2005年 | 74篇 |
2004年 | 55篇 |
2003年 | 57篇 |
2002年 | 54篇 |
2001年 | 29篇 |
2000年 | 35篇 |
1999年 | 35篇 |
1998年 | 20篇 |
1997年 | 28篇 |
1996年 | 43篇 |
1995年 | 54篇 |
1994年 | 44篇 |
1993年 | 39篇 |
1992年 | 45篇 |
1991年 | 34篇 |
1990年 | 25篇 |
1989年 | 30篇 |
1988年 | 22篇 |
1987年 | 18篇 |
1986年 | 21篇 |
1985年 | 18篇 |
1984年 | 16篇 |
1983年 | 3篇 |
1982年 | 15篇 |
1981年 | 17篇 |
1980年 | 20篇 |
1979年 | 18篇 |
1978年 | 18篇 |
1977年 | 14篇 |
1976年 | 7篇 |
1973年 | 6篇 |
1969年 | 2篇 |
排序方式: 共有1968条查询结果,搜索用时 15 毫秒
1.
Hai-Yan Zhou Erdjan Salih Melvin J. Glimcher 《The Journal of biological chemistry》2010,285(46):36170-36178
Past studies of bone extracellular matrix phosphoproteins such as osteopontin and bone sialoprotein have yielded important biological information regarding their role in calcification and the regulation of cellular activity. Most of these studies have been limited to proteins extracted from mammalian and avian vertebrates and nonvertebrates. The present work describes the isolation and purification of two major highly glycosylated and phosphorylated extracellular matrix proteins of 70 and 22 kDa from herring fish bones. The 70-kDa phosphoprotein has some characteristics of osteopontin with respect to amino acid composition and susceptibility to thrombin cleavage. Unlike osteopontin, however, it was found to contain high levels of sialic acid similar to bone sialoprotein. The 22-kDa protein has very different properties such as very high content of phosphoserine (∼270 Ser(P) residues/1000 amino acid residues), Ala, and Asx residues. The N-terminal amino acid sequence analysis of both the 70-kDa (NPIMA(M)ETTS(M)DSKVNPLL) and the 22-kDa (NQDMAMEASSDPEAA) fish phosphoproteins indicate that these unique amino acid sequences are unlike any published in protein databases. An enzyme-linked immunosorbent assay revealed that the 70-kDa phosphoprotein was present principally in bone and in calcified scales, whereas the 22-kDa phosphoprotein was detected only in bone. Immunohistological analysis revealed diffusely positive immunostaining for both the 70- and 22-kDa phosphoproteins throughout the matrix of the bone. Overall, this work adds additional support to the concept that the mechanism of biological calcification has common evolutionary and fundamental bases throughout vertebrate species. 相似文献
2.
Chelsea E. Matisz Cameron P. Goater Douglas Bray 《International journal for parasitology》2010,40(3):307-312
Evidence for the presumed linkage between the enigmatic rodlet cells of fish and exposure to helminths is anecdotal and indirect. We evaluated the proliferation and development of rodlet cells in the optic lobes of fathead minnows exposed to cercariae of Ornithodiplostomum ptychocheilus. Mean rodlet cell densities (ca. 10/mm2) in the optic lobes were similar between unexposed controls and minnows with 1- and 2-week old infections. Rodlet cell densities increased at 4 weeks p.i., reaching maxima (ca. 200/mm2) at 6 weeks p.i., followed by a decline at 9 weeks. This temporal pattern of proliferation and maturation paralleled the development of metacercariae within the optic lobes. Unencysted metacercariae develop rapidly within tissues of the optic lobes for approximately 4 weeks after penetration by cercariae, then shift to the adjacent meninges to encyst. The former stage is associated with tissue damage, the latter with massive inflammation of the meninges. Thus, peak densities and maturation of rodlet cells correspond to the period when inflammation of the meninges caused by the large metacercarial cysts is at a maximum. Our results support recent contentions that rodlet cells comprise part of the host inflammatory defence response. 相似文献
3.
Shallow-water vegetated estuarine habitats, notably seagrass, mangrove and saltmarsh, are known to be important habitats for
many species of small or juvenile fish in temperate Australia. However, the movement of fish between these habitats is poorly
understood, and yet critical to the management of the estuarine fisheries resource. We installed a series of buoyant pop nets
in adjacent stands of seagrass, mangrove and saltmarsh in order to determine how relative abundance of fishes varied through
lunar cycles. Nets were released in all habitats at the peak of the monthly spring tide for 12 months, and in the seagrass
habitat at the peak of the neap tide also. The assemblage of fish in each habitat differed during the spring tides. The seagrass
assemblage differed between spring and neap tide, with the neap tide assemblage showing greater abundances of fish, particularly
those species which visited the adjacent habitats when inundated during spring tides. The result supports the hypothesis that
fish move from the seagrass to the adjacent mangrove and saltmarsh during spring tides, taking advantage of high abundances
of zooplankton, and use seagrass as a refuge during lower tides. The restoration and preservation of mangrove and saltmarsh
utility as fish habitat may in some situations be linked to the proximity of available seagrass. 相似文献
4.
Summary The effect of 16 weeks total starvation on the ultrastructure of the red and white myotomal muscles of the crucian carp (Carassius Carassius) has been investigated.
In the white fibres the amount of myofibrillar material fell from 89.6% to 70.7% of the total fibre volume whilst in the red
fibres the fall was from 72.2% to 70.3%. The sarcoplasmic reticulum appeared to have become swollen during starvation in both
fibre types. In the white fibres the terminal cisternae of some triads seem to have fused. The volume of the red fibres occupied
by mitochondria was reduced from 16.2 % to 5.9 %. The concentration of mitochondria in the white fibres was too low to detect
any quantitative changes. A marked reduction in the amount of euchromatin material was observed in most white fibre nuclei
and many red fibre nuclei.
Many of the ultrastructural changes noted in the present study can be correlated with biochemical changes known to occur in
the red and white myotomal muscles of fish during starvation.
This work was supported by a grant from the Natural Environmental Research Council. 相似文献
5.
To produce many small or few large eggs: a size-independent reproductive tactic of fish 总被引:2,自引:2,他引:0
Summary We demonstrate here the existence of a range of size-independent reproductive tactics in teleostean fish involving the allocation of a size-dependent reproductive effort between fecundity and egg size. Despite considerable evidence that larger eggs and the larvae hatching from them are more likely to survive than smaller ones, we found no evidence of evolutionary trends towards greater egg sizes. Fish with pelagic eggs tend to spawn many, and therefore small, eggs, whereas demersal spawners tend to produce large, and therefore few, eggs. Maximizing egg number should increase the number of eggs hatching in suitable locations in the patchy pelagic environment and, hence, increase the reproductive success of pelagic spawners. On the other hand, the reproductive success of demersal spawners, which reduce the variance in growing conditions experienced by the off spring, should be more dependent on the survival of the individual larvae, which increases as egg size increases. 相似文献
6.
Synopsis The bloater, Coregonus hoyi, deposits its eggs on deep sediments (70–100 m in Lake Michigan), where its eggs and embryos can be exposed to epibenthic
predators. We investigated the vulnerability of early life intervals of bloaters to predation by mysids, Mysis relicta, which are mostly epibenthic by day and planktonic at night. Bloaters were raised from spawn in the laboratory and presented
to field-collected mysids in laboratory predation trials. Eggs were not ingested by the mysids. Embryonic bloaters were vulnerable
to predation by mysids only during the interval between hatching and swim up, usually 1–24 h under laboratory conditions.
The mysids required about a day of exposure to this novel prey before they were able to kill significant numbers of the bloater
embryos by making successive attacks with their thoracic legs. In experiments with experienced (2 and 3 days with bloater
embryos) mysids, a functional response between embryo density and mysid predation rates was apparent. Temperature and the
presence of alternative prey (zooplankton) did not alter the ‘kill rate’ (about 2.5 embryos mysid-1d-1) of experienced mysids at high bloater densities (>4 bloaters/mysid). However, more embryos were partially, rather than completely,
ingested at 4 versus 9° C and in the presence of zooplankton. 相似文献
7.
Fish size and habitat depth relationships in headwater streams 总被引:5,自引:0,他引:5
Summary Surveys of 262 pools in 3 small streams in eastern Tennessee demonstrated a strong positive relationship between pool depth and the size of the largest fish within a pool (P<0.001). Similarly, the largest colonizers of newly-created deep pools were larger than the colonizers of shallow pools. We explored the role of predation risk in contributing to the bigger fish — deeper habitat pattern, which has been noted by others, by conducting five manipulative field experiments in two streams. Three experiments used stoneroller minnows (Campostoma anomalum); one used creek chubs (Semotilus atromaculatus); and one used striped shiners (Notropis chrysocephalus). The stoneroller experiments showed that survival of fish approximately 100 mm in total length (TL) was much lower in shallow pools (10 cm deep) than in deep (40 cm maximum) pools (19% versus 80% survival over 12 d in one experiment) and added cover markedly increased stoneroller survival in shallow pools (from 49% to 96% in an 11-d experiment). The creek chub experiment showed that, as for stonerollers, pool depth markedly influenced survival: the chubs survived an average of 4.9 d in shallow pools and >10.8 d in deep pools. In the striped shiner experiment in shallow artificial streamside troughs, no individuals 75–100 mm TL survived as long as 13 d, where-as smaller (20–25 mm) fish had 100% survival over 13 d. The results of the experiments show that predation risk from wading/diving animals (e.g., herons and raccoons) is much higher for larger fishes in shallow water than for these fishes in deeper water or for smaller fish in shallow water. We discuss the role of predation risk from two sources (piscivorous fish, which are more effective in deeper habitats, and diving/wading predators, which are more effective in shallow habitats) in contributing to the bigger fish — deeper habitat pattern in streams. 相似文献
8.
Subcellular modifications in hepatocytes of Carassius carassius var. auratus subjected to 24 hr and 48 hr sublethal acute lead (5mg·1−1) exposure were studied by electron microscopy. Cytological alterations were observed after 24 hr of treatment and became more evident after 48 hr. Lead induced an increase in nuclear heterochromatin and alterations in mitochondria, endoplasmic reticulum and Golgi complex ultrastructure. Glycogen granula decreased, and secondary lysosomes and lipid droplets increased. Furthermore, intracytoplasmic lumina with microvilli-bearing surfaces and numerous autophagic vacuola were observed after 48 hr of exposure. 相似文献
9.
Synopsis Developmental patterns as seen in cyprinodontiforms fishes with different reproductive styles are compared, and discussed
in relation to ecology and evolutionary significance. The discussion centres around Adinia xenica (its detailed ontogeny presented in two previous sequels to this paper), and, from the existing literature, Fundulus heteroclitus (closely related), Austrofundulus myersi (an annual) and Platypoecilus maculatus (a livebearer). The embryonic resting interval is present in various forms in the first three species, and differences in
it and the overall patterns of development are shown to be consistent with ecological conditions. Termination of the resting
interval leads immediately to hatching, a process in A. xenica, as in F. heteroclitus, apparently initiated by the appropriate summation of internal and external factors. These factors include any or all of:
metabolic changes and increased oxygen requirements, response to light, reduced environmental oxygen, agitation, and increased
hydrostatic pressure. They all can cause increased movement by the embryo which is credited with rupturing hatching gland
cells and releasing the enzyme(s). Annual fishes experience 3 pronounced resting intervals, termed diapauses. These are discussed
in the context of apparent steps and thresholds, and evolutionary ecology. A possible evolutionary sequence, from a simple
fractional spawning pattern to diapause, is presented. Morphological differences in primary embryonic respiratory surfaces,
as seen in the four species, are related to environmental conditions. The above illustrate ways in which the same basic structures
and events are modified to cope with different habitats. 相似文献
10.
E. Follenius 《Cell and tissue research》1970,106(1):61-68
Résumé Dans l'hypothalamus de l'Epinoche, certains prolongements nerveux présentent une organisation particulière du réticulum endoplasmique. Des séries de vésicules interconnectées sont disposées en stries transverses espacées de 0,3 à 1,3 . Cette organisation particulière du réticulum endoplasmique existe dans les prolongements nerveux de fort diamètre (1 ), même dans les fibres neurosécrétrices.
Special organization of the endoplasmic reticulum in some hypothalamic nerve processes ofGasterosteus aculeatus
Summary In some nerve processes of the hypothalamus ofGasterosteus aculeatus, the endoplasmic reticulum displays a special organization. Small interconnected smooth vesicles constitute transverse lines spaced between 0.3 and 1.3 . This special organization of the endoplasmic reticulum has been found in nerve processes with a diameter larger than 1 even in neurosecretory ones.相似文献