Ethephon and auxin induce mycorrhiza-like changes in the morphology of root organ cultures of Mugo pine

The effects of ethylene and auxin on the morphology and anatomy of root organ cultures of Pinus mugo Turra var. mugo were investigated to test the hypothesis that changes in root morphology associated with formation of ectomycorrhizae may be related to ethylene produced by ectomycorrhizal fungi or by host plant roots in response to fungus-produced auxin. Morphological changes characteristic of mycorrhizal infection include dichotomous branching of lateral roots, inhibition of root hair formation and enlargement of cortical cells. Lateral roots on non-mycorrhizal root organ cultures, grown in a defined medium, underwent dichtotomous branching while root hair formation was inhibited in response to the ethylene released by 50 and 100 μ M ethephon (2-chloroethylphosphonic acid), but no effect on cortical cell dimensions was observed. The auxin, naphthaleneacetic acid (1 and 10 μ M ) also stimulated dichotomous branching and inhibited root hair formation, but to a lesser extent and with a greater lag time than ethephon. Auxin-stimulated ethylene production by root organ cultures was demonstrated. This appeared to be responsible, at least in part, for the auxin-induced dichotomous branching since the ethylene action inhibitor, silver thiosulfate (0.1 m M ) inhibited the response to auxin by 35%.     

Alterations of the Characteristics of the Circadian Rest‐Activity Rhythm of Cancer In‐Patients

The aim of the present study was to evaluate the characteristics of the circadian rest‐activity rhythm of cancer patients. Thirty‐one in‐patients, consisting of 19 males and 12 females, were randomly selected from the Regional Cancer Center, Pandit Jawaharlal Nehru Medical College, Raipur, India. The rest‐activity rhythm was studied non‐invasively by wrist actigraphy, and compared with 35 age‐matched apparently healthy subjects (22 males and 13 females). All subjects wore an Actiwatch (AW64, Mini Mitter Co. Inc., USA) for at least 4–7 consecutive days. Fifteen‐second epoch length was selected for gathering actigraphy data. In addition, several sleep parameters, such as time in bed, assumed sleep, actual sleep time, actual wake time, sleep efficiency, sleep latency, sleep bouts, wake bouts, and fragmentation index, were also recorded. Data were analyzed using several statistical techniques, such as cosinor rhythmometry, spectral analysis, ANOVA, Duncan's multiple‐range test, and t‐test. Dichotomy index (I<O) and autocorrelation coefficient (r24) were also computed. The results validated a statistically significant circadian rhythm in rest‐activity with a prominent period of 24 h for most cancer patients and control subjects. Results of this study further revealed that cancer patients do experience a drastic alteration in the circadian rest‐activity rhythm parameters. Both the dichotomy index and r24 declined in the group of cancer patients. The occurrence of the peak (acrophase, Ø) of the rest‐activity rhythm was earlier (p<0.001) in cancer patients than age‐ and gender‐matched control subjects. Results of sleep parameters revealed that cancer patients spent longer time in bed, had longer assumed and actual sleep durations, and a greater number of sleep and wake bouts compared to control subjects. Further, nap frequency, total nap duration, average nap, and total nap duration per 1 h awake span were statistically significantly higher in cancer patients than control subjects. In conclusion, the results of the present study document the disruption of the circadian rhythm in rest‐activity of cancer in‐patients, with a dampening of amplitude, lowering of mean level of activity, and phase advancement. These alterations of the circadian rhythm characteristics could be attributed to disease, irrespective of variability due to gender, sites of cancer, and timings of therapies. These results might help in designing patient‐specific chronotherapeutic protocols.     

Specific intercalary growth of rhizophores and roots in Selaginella kraussiana (Selaginellaceae) is related to unique dichotomous branching

Rhizophores - leafless axial organs, which apices are the only sites for root formation - are unique to the Selaginella genus. Both rhizophores and roots are dichotomously branched, forming a multibranch rhizophore-root system. In the examined Selaginella kraussiana, the first dichotomous division of a rhizophore results in the initiation of the twin root meristems, giving rise to the root primordia. These primordia are temporarily arrested in growth, but at the same time they are elevated, hidden inside the rhizophore apex due to the activity of a meristematic zone at the rhizophore axis. This meristematic region, located basally to the root meristems, apart from the rhizophore tissues consists of the derivative cells of root primordia and may be considered as a specific intercalary meristem.The growth mode of the roots is similar to that of the rhizophore, including dichotomous branching of the meristem and a temporary developmental arrest of the subsequent root primordia. The reiteration of the developmental program also involves the formation of new intercalary zones, thus through divisional activity, the root with dichotomized apex grows for some time as a single axis and, then the young roots emerge. At each step of the rhizophore-root branch system formation, tissues of the parental axis contribute significantly to the growth and emergence of the next axes. Therefore, dichotomous branching in Selaginella appears to be exceptional in comparison with other known dichotomies of axial organs.     

Dense crowns of the hemiparasitic mistletoe Viscum album L. exhibit shrub-like growth and high dry matter turnover

Growth characteristics and crown parameters of the evergreen, hemiparasitic mistletoe Viscum album were studied using a growth model based on the regular dichotomous growth and conservancy of branching patterns. Data were collected from one final harvest of 13 individuals of three different subspecies growing on the hosts Betula pendula, Abies alba and Pinus sylvestris. Compared to Viscum album ssp. album growing on deciduous hosts, the two subspecies abietis and austriacum (syn. laxum) on evergreen hosts showed longer average leaf life spans, but leaves continuously increased in area over time up to an age of at least 4 years irrespective of host. Relative growth rates in plant dry matter (RGR; 0.53–0.61 g g⁻¹ a⁻¹) were in the same range as of deciduous shrubs in central Europe but clearly higher than in mature forest trees. However, this growth is hidden within the spherical crowns of mistletoes, as annual increase in crown volume is extraordinarily low, resulting in very high leaf area densities (LAD; 7.7–17.6 m² m⁻³), not found in any other woody species. Besides differences in leaf size and phenology, the three subspecies exhibit similar patterns of partitioning. As in other mistletoe species, significant parts of dry matter are regularly allocated into fruit production (10–15%). Regarding the generally lower net photosynthesis rates in mistletoes as compared to their hosts, it is questioned, whether photoautotrophic carbon gain of the leaves is sufficient to maintain the observed high relative growth rates.     

关于“中国旧石器时代中期”的探讨

本文探讨了中国旧石器时代中期考古学研究的若干问题,包括研究历史、资料现状、年代学手段、石器技术的特点及发展变化,以及与西方旧石器时代考古学的历史渊源及现状比较。通过对“中国旧石器时代中期”这一术语的来源及其所涵盖的时限及文化内涵的演变,对这一时代界定所采取的原则和方法,以及中国旧石器时代文化特点的考察,作者认为“中国旧石器时代中期”不是一个严格和有意义的学术概念,因而中国旧石器时代考古学应摒弃传统的3期断代模式,而改为早、晚两期的2分法。文章并对中国旧石器时代早期延续时间的长久和晚期出现新的文化内容的动因进行了讨论。     

具有周期强迫项的三分子反应模型的概周期解

研究具有周期强迫项的三分子反应模型x=a-bx-xy2,y-bx+xy2-y+c+δcoswt的概周期解的存在性.这里δcoswt为周期强迫项,常数a,b,c是与某化学元素浓度有关的常数.