蝮蛇毒抗凝血活酶组份及几种蛇毒粗毒对人红细胞和血小板的作用

蝮蛇毒抗凝血活酶组分及蝗蛇毒、圆斑蝰蛇毒和眼镜蛇毒粗毒,不仅能够水解血浆中的磷脂,而且还能水解完整人红细胞膜和完整人血小板膜上的磷脂。但是五步蛇毒和金环蛇毒粗毒却不能水解完整人血小板膜上的磷脂。 扫描电镜观察表明,由于抗凝血活酶组份和几种蛇毒粗毒的作用,人红细胞和人血小板的形态发生了巨大的变化;人红细胞由正常的双圆盘形变成带刺的小球,人血小板的外形变成蜂窝状。     

蝮蛇抗栓酶治疗小儿脑炎致急性偏瘫血中6—K—PGF1α,TXB2的变化

应用蝮蛇抗栓酶治疗小儿脑炎致急性偏瘫共25例。沿疗前后检测血中的6-K-PGF_1x及 TXB_2.结果表明:治疗后血中 6-酮-前列腺素 F_1x 明显增高,血栓素 B_2明显降低。推测蝮蛇抗栓酶之所以具有去纤、溶栓、扩血管等作用,可能与血中前列腺素、血栓素 B_2水平变化有关。     

Crystal structure determination of basic phospholipase A2 from venom of Agkistrodon halys pallas by molecular replacement method

Basic phospholipase A₂ (BPLA₂) from the venom of Agkistrodon halys pallas has a strong ability to hemolyze erythrocytes. The asymmetrical unit of P2₁2₁2₁ crystal of BPLA₂ contains two molecules. Self-rotation function was used to study the orientation relationship of these two molecules. Cross-rotation and translation functions were then used to determine the orientations and positions of the two molecules in the unit cell. The model building and preliminary structure refinement were carried out. The result shows that the two molecules in the asymmetrical unit of orthorhombic crystal are related by a non-crystallographic 2-fold symmetry axis.     

蝮蛇毒碱性磷脂酶A_2基因的克隆

从蝮蛇毒腺中抽提总ＲＮＡ．利用人工合成寡核苷酸引物作逆转录,以ｃＤＮＡ为模板进行体外扩增,获得磷脂酶Ａ２（简称ＰＬＡ２）基因,克隆至ｐＢＳ－ｋｓ载体中。通过对３个碱性ＰＬＡ２（简称ＢＰＬＡ２）基因单独克隆分别作ＤＮＡ全序列分析,推导ｐｒｏ－ＢＰＬＡ２由１３８个氨基酸残基构成,与已测定的部分氨基酸序列比较,基本相符。该基因成功的克隆,不仅推导出ＢＰＬＡ２的蛋白质全序列,也为进一步开展蛇毒功能肽蛋白质工程的研究工作打下了良好的基础。     

五步蛇主要数量性状与排毒量的相关分析

本文首次报道了五步蛇身体主要部位的数量性状与其排毒量的关系。其中我们发现与排毒量有关的部位是头积、体粗、体重、佛指长。     

尖吻蝮咬伤与心脏损害：附20例分析

本文报告尖吻蝮咬伤并心脏受累20例。经中西医结合治愈19例(95％),死亡1例(5％),并对其发病机理及治疗进行了讨论。     

Terbium(III) fluorescence probe studies on metal ion-binding sites in anticoagulation factor I from Agkistrodon acutus venom

Anticoagulation factor I (ACF I) isolated from the venom of Agkistrodon acutus is an activated coagulation factor X-binding protein with marked anticoagulant activity. Present studies show that holo-ACF I assumes a compactly folded structure in the range of pH 5–6, in which the most interior Trp residues and quenchers are adjacent. Tb³⁺ ions can completely replace both Ca²⁺ ions in holo-ACF I, as determined by equilibrium dialysis. Although the two Tb³⁺ ions in Tb³⁺-ACF I have slightly different luminescence efficiencies, both have similar quenching effects on the intrinsic fluorescence, suggesting that probably there are same numbers of Trp residues close to both Tb³⁺-binding sites. Two Tb³⁺-binding sites with similar apparent Tb³⁺ association constant values, (1.69 ± 0.02) × 10⁷ M^–1 and (1.42 ± 0.01) × 10⁷ M^–1, respectively, were further identified through Tb³⁺ fluorescence titration. In addition, it has been confirmed from the titration of holo-ACF I and Tb³⁺-ACF I with NBS that only interior Trp residues are involved in the energy transfer to Tb³⁺ ions and that all accessible Trp residues located in the surface of holo-ACF I have similar affinity to NBS, while those located in the surface of Tb³⁺-ACF I have two different kinds of affinity to NBS, which strongly suggests a conformational change of holo-ACF I upon substitution of Tb³⁺ for Ca²⁺. The results show that although the Tb³⁺-altered conformation of ACF I cannot support the binding of Tb³⁺-ACF I with FXa, determined by nondenaturing PAGE, Tb³⁺ ions are effective and useful fluorescence probes to analyze the structures and properties of Ca²⁺-binding sites in ACF I.     

三七皂甙在五步蛇伤中的治疗作用

目的探讨三七皂甙对五步蛇咬伤的治疗作用。方法将2011年4～11月我院收治的五步蛇咬伤3h内就诊的患者30例随机分为对照组（A组）21例,给予常规治疗;研究组（B组）9例,在常规治疗的基础上加用三七皂甙治疗。观察比较两组患者24h后磷酸肌酸激酶的变化以及肿胀程度的改善情况。结果B组24h后磷酸肌酸激酶较A组下降,肿胀程度比A组减轻,差异均有显著统计学意义（P〈0．05）。结论三七皂甙对五步蛇咬伤的疗效明显,值得临床推广。     

蛇毒清合剂治疗蝮蛇咬伤的疗效观察

目的观察蛇毒清合剂对蝮蛇咬伤的临床疗效。方法选择2008年11月～2011年11月我院收治的蝮蛇咬伤患者62例,随机分成两组,其中观察组31例,在常规治疗的基础上加服蛇毒清合剂,对照组31例采用常规治疗。比较两组患者血清酶学3项（CK、LDH、AST）、肿胀消退时间、疼痛缓解情况、肾功能衰竭、呼吸衰竭发生率、局部组织坏死发生率、致残率以及住院时间。结果观察组的血清酶学3项（CK、LDH、AST）、肿胀消退时间、疼痛缓解情况、肾功能衰竭、呼吸衰竭发生率、局部组织坏死发生率、致残率以及住院时间均明显优于对照组（均P〈0.05）。结论蛇毒清合剂治疗对蝮蛇咬伤有显著疗效。