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1 引言 柳牡蛎蚧(Lepidosaphes salicina Bersch)(同翅目:盾蚧科)是一种以为害杨树为主的刺吸式害虫之一,广泛分布于“三北”防护林区,该蚧一年发生一代,以卵越冬。通过刺吸式口器刺入树皮内,吸取树木养分和水分,并使树木表皮栓化。同时,由于枝干被蚧壳所覆盖,对呼吸及光合作用也有影响,特别是幼树被害后,一般 相似文献
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海鲜是全球超过30亿人的主要蛋白质来源,占人类饮食中动物蛋白的16%左右。目前,全球超过一半的海鲜来自水产养殖,水产养殖业每年产生的经济效益高达2435亿美元,已成为全球增长最快的食品生产方式(年增长约6%)。未来随着全球人口的增长,预计人类对海鲜的需求将翻倍增加,水产养殖业也将持续保持增长,成为未来海鲜供应的重要保障。 相似文献
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以8个敏感菌株作为指示菌,采用管碟法对分离自湛江硇洲岛(20°52′N~20°56′N,110°33′E~110°38′E)潮汐带香港巨牡蛎(Crassostrea hongkongensis)中的72株细菌的发酵液进行抗菌筛选,并对阳性菌株进行基因组DNA提取、16S rRNA基因PCR扩增和序列测定,继而进行系统发育分析。抗菌实验结果表明,受试菌株中有23株菌的发酵产物具有抗菌活性(阳性率31.9%),其中有5个菌株(JSM 111024、JSM 111027、JSM 111029、JSM 111076、JSM 111083)具有较强的抗菌活性。基于16S rRNA基因序列的系统发育分析表明,这23株菌具有较高的类群多样性和物种多样性,属于3个系统发育群/门(Alpha Proteobacteria、Gamma Proteobacteria、Bacteroidetes)中的8个科(Aeromonadaceae、Flavobacteriaceae、Halomonadaceae、Idiomarinaceae、Phyllobacteriaceae、Pseudoalteromonadaceae、Shewanellaceae、Xanthomonadaceae)的8个属(Idiomarina、Halomonas、Myroides、Nitratireductor、Oceanimonas、Pseudoalteromonas、Shewanella、Wohlfahrtiimonas),可分为11个物种。优势类群为Gamma Proteobacteria亚门(14株),其中优势属为Oceanimonas属(6株);第二大类群为Bacteroidetes门(7株),都属于Flavobacteriaceae科的Myroides属。具有较强抗菌活性的5个菌株中,有4个菌株(JSM 111024、JSM 111027、JSM 111029、JSM 111083)属于Alpha Proteobacteria 亚门Phyllobacteriaceae科〖WTBX〗Oceanimonas属,而菌株JSM 111076属于Gamma Proteobacteria 亚门Aeromonadaceae科Nitratireductor属。 相似文献
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从近江牡蛎(Jinjiang Ostrea vivularisGould)肠道中分离出21株菌,其中9株革兰氏阳性菌,12株革兰氏阴性菌。并检测其蛋白酶、脂肪酶、淀粉酶、纤维素酶的产酶能力。结果表明,13株菌(61.9%)能分泌蛋白酶,13株(61.9%)分泌淀粉酶;11株(52.4%)产脂肪酶;7株(33.3%)产纤维素酶。产4种酶的有5株,产3种酶的3株,产2种酶的5株,产1种酶的3株,不产酶的仅有5株。产酶菌株的比例高达76.2%(16/21),可见近江牡蛎肠道细菌对食饵消化有重要作用。 相似文献
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Qiaoli Feng ;Zi Fang ;Zhenguang Yan ;Rui Xing ;Liping Xie ;Rongqing Zhang 《Acta biochimica et biophysica Sinica》2009,(11):955-962
We previously identified a matrix protein, MSI7, from pearl oyster Pinctada fucata. According to the structural analysis, the DGD site in the N-terminal of MSI7 is crucial for its role in the shell formation. In this study, we expressed a series of recombinant MSI7 proteins, including the wild-type and several mutants directed at the DGD site, using an Escherichia coli expression system to reveal the structure-function relationship of MSI7. Furthermore, in vitro crystallization, crystallization speed assay, and circular dichroism spectrometry were carried out. Results indicated that wild-type MSI7 could induce the nucleation of aragonite and inhibit the crystallization of calcite. However, none of the mutants could induce the nucleation of aragonite, but all of them could inhibit the crystallization of calcite to some extent. And all the proteins accelerated the crystallization process. Taken together, the results indicated that MSI7 could contribute to aragonite crystallization by inducing the nucleation of aragonite and inhibiting the crystallization of calcite, which agrees with our prediction about its role in the nacreous layer formation of the shell. The DGD site was critical for the induction of the nucleation of aragonite. 相似文献