Identification of an apyrase activating protein and of calmodulin in Solanum tuberosum

Three types of effector proteins have been isolated from a partially purified protein preparation of potato tuber. One of the proteins is a typical calmodulin which has no effect on apyrase. The two other proteins modulate ATPase and ADPase activities; one of them with an activating and the other with an inhibitory effect on apyrase. Calmodulin from potato tuber purified to homogeneity had a M_r of 17 500 and an isoelectric point of 4.4. Although the apyrase activating protein is not a pure fraction it differs from calmodulin because unlike this protein it is independent of calcium and does not activate cyclic nucleotide phosphodiesterase from bovine heart. Treatment of the activating protein with tetranitromethane reduces its effect on apyrase, while no change was detected upon treatment with bisdithionitrobenzoic acid.     

Ganoderic acid derivatives and ergosta-4,7,22-triene-3,6-dione from Ganoderma lucidum

Seven new triterpenoids, ganoderic acid T, ganoderic acid S, ganoderic acid R, ganoderic acid P, ganoderic acid Q, ganoderic acid 0, 7-O-methyl-ganoderic acid 0 and a known ergosterol derivative, ergosta-4,7,22-triene-3,6-dione were isolated from the cultured mycelium of Ganoderma lucidum. The structure of the first compound was determined using spectroscopic and X-ray analysis, and the structures of the other compounds were elucidated by spectroscopic data.     

Revision of the structures of rhazicine and rhazimine,two alkaloids from Melodinus acutiflorus

From the leaves of Melodinus acutiflorus the two alkaloids rhazicine and rhazimine were isolated and identified with known substances. However, based on extensive spectral and X-ray analysis their structures published earlier have to be revised.     

A novel withanolide from Datura metel

A novel withanolide, daturilin, has been isolated from the alcohol soluble extract of the fresh leaves of D. metel and its structure established as 1-oxo-21,24S-epoxy- (20S,22S)-witha-2,5,25-trienolide through spectral studies including 2D NMR.     

Phenolic glycosides from Lilium longiflorum

Three bitter principles were isolated from the bulb scales of Lilium longiflorum and identified as 3,6′-diferuloylsucrose, 4-acetyl-3,6′-diferu     

Biotransformation of isopapaverine by Silene alba cell suspensions

Silene alba cell suspension was supplied with isopapaverine (methanesulphonate), a papaverine analogue. The mechanisms of biotransformation were id     

Diterpenes of Ericameria linearifolia

The isolation and characterization of two new bicyclic labdane diterpenes, 18α-succinyloxy-labd-7-en-15-oic acid and 8,17H-7,8-dehydropinifolic acid,     

Metabolism of limonoids in the citrus hybrid calamondin

Radioactive tracer work showed that in young seeding of calamondin decetylnomilinate was converted to nomilin, obacunone and 6-keteb-nomilol, a new lim     

Alkaloids from callus cultures of Holarrhena floribunda

From callus cultures ofHolarrhena floribunda a complex mixture of alkaloids has been isolated in small yield, the main alkaloid of which was identified as conessine by MS, GC and TLC.