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991.
Xin-Hua Liu Jing Zhu An-na Zhou Bao-An Song Hai-Liang Zhu Lin-Shan bai Pinaki S. Bhadury Chun-Xiu Pan 《Bioorganic & medicinal chemistry》2009,17(3):1207-1213
A series of new 2-(1-(2-(substituted-phenyl)-5-methyloxazol-4-yl)-3-(2-substitued-phenyl)-4,5-dihydro-1H-pyrazol-5-yl)-7-substitued-1,2,3,4-tetrahydroisoquinoline derivatives were synthesized. The results showed that compounds 9q and 10q can strongly inhibit Staphylococcus aureus DNA gyrase and Bacillus subtilis DNA gyrase (with IC50s of 0.125 and 0.25 μg/mL against S. aureus DNA gyrase, 0.25 and 0.125 μg/mL against B. subtilis DNA gyrase). On the basis of the biological results, structure–activity relationships were also discussed. 相似文献
992.
Marcel Aribaud Michel Noirot Anne Gauvin Christine Da Silva-Robert Isabelle Fock Hippolyte Kodja 《Plant Physiology and Biochemistry》2009,47(4):313-321
Calli induced from Solanum torvum stem explants were inoculated with Ralstonia solanacearum under partial vacuum. All calli showed a hypersensitive response after infiltration. Furthermore, amine oxidase activity with aldehyde and H2O2 production was detected in semi-purified cell walls of calli infiltrated by the bacteria. Due to its preferential affinity for monoamines, this enzyme is supposed to have monoamine oxidase-like (MAO-like) activity. Moreover, the presence of hydroxyl radicals in the aromatic cycle alters the oxidative deamination kinetics of potential substrates. Indeed, the oxidation of dopamine (+2, OH) was shown to be faster than that of tyramine (+1, OH), which in turn was faster than that of phenylethylamine (0, OH). The MAO-like catalytic activity was significantly inhibited by some reducing agents such as sodium bisulphite and cysteine, and also by tryptamine under anaerobiosis. This latter result suggested that the prosthetic group of the MAO-like enzyme could be a tyrosine-derived 6-hydroxytopaquinone structure. Finally, the sigmoid kinetics of the MAO-like enzyme in semi-purified cell walls did not correspond to that expected for a purified MAO, suggesting that the kinetics were affected by some factors present in cell walls. 相似文献
993.
记述了采自黑龙江省伊春市呼兰河的中国软滑水螨一新纪录属:妖水螨属嘞Tiphys Koch,1836,及1新种:伊春妖水螨 Tiphys yichunensis sp.nov,.新种与分布于俄罗斯的近疤妖水螨Tiphys scaurellus Tuzovskij,1983近似,区别在于:新种P-Ⅳ背面起始端亚中部具1栓毛,近疤妖水螨缺失;新种足雄螨足Ⅲ爪特化,其中一爪为钩状简单爪,另一爪中、基部膨大,近疤妖水螨雄螨足Ⅲ爪均具腹小爪;新种雄螨Ⅳ.L4近筒状,近疤妖水螨雄螨Ⅳ.L-4从基部至端部逐渐膨大. 相似文献
994.
台湾窗翅叶蝉属种类纪要(半翅目,叶蝉科,大叶蝉亚科) 总被引:2,自引:0,他引:2
记述台湾的窗翅叶蝉属Mileewa Distant 11种,其中包括3新种:褐条窗翅叶蝉M.fusciovittata sp.nov.,南投窗翅叶蝉M.nantouensis sp.nov.和尖头窗翅叶蝉M.sharpa sp.nov.,并确认双斑窗翅叶蝉M.bimaculata Cai et He是船茎窗翅叶蝉M.ponta Yang et Li的新异名.文中详细描述了新种的形态特征并绘制了雄性外生殖器构造图,提供了11种的整体图片和分布,编制了分种检索表.研究标本分别保存在贵州大学昆虫研究所(GUGC)、台湾国立自然科学博物馆(NMNS)、台湾农业试验所(TARI)和台湾国立中兴大学(NCHU). 相似文献
995.
996.
采用19,22,25,28和31℃5个温度对竹织叶野螟Algedonia coclesalis Walker各虫态(龄)发育起点温度和有效积温进行测定。结果表明,竹织叶野螟的在19~31℃范围内均能正常生长发育,尤其是28~31℃范围最适宜于竹织叶野螟的生长发育。卵、1龄幼虫、2龄幼虫、3龄幼虫、4龄幼虫、5龄幼虫、6龄幼虫、7龄幼虫、蛹、成虫及世代的发育起点温度分别为6.63,12.51,11.18,10.93,10.05,8.01,6.80,5.78,6.20,7.81和8.33℃,有效积温分别为124.19,64.54,72.59,82.08,93.46,136.84,155.42,201.06,211.55,111.49和1235.50日.度。 相似文献
997.
Patricia E. López-Calcagno Johanna Moreno Luis Cedeño Luis Labrador Juan L. Concepción Luisana Avilán 《Mycological Research》2009,113(6-7):771-781
The genes of the mitochondrial and cytosolic malate dehydrogenase (mMDH and cMDH) of Phytophthora infestans were cloned and overexpressed in Escherichia coli as active enzymes. The catalytic properties of these proteins were determined: both enzymes have a similar specific activity. In addition, the natural mitochondrial isoenzyme was semi-purified from mycelia and its catalytic properties determined: the recombinant mitochondrial isoform behaved as the natural enzyme. A phylogenetic analysis indicated that mMDH, present in all stramenopiles studied, can be useful to study the relationships between these organisms. MDH with the conserved domain MDH_cytoplasmic_cytosolic is absent in some stramenopiles as well as in fungi. This enzyme seems to be less related within the stramenopile group. The Phytophthora cMDHs have an insertion of six amino acids that is also present in the stramenopile cMDHs studied, with the exception of Thalassiosira pseudonana cMDH, and is absent in other known eukaryotic cMDHs. 相似文献
998.
999.
《Environmental and Experimental Botany》2006,55(1-2):201-208
In our previous work, we described the defensive potential of a wound- and methyl jasmonate-inducible 87 kDa tomato cystatin and its accumulation in a crystalline form. Here, we report the immunolocalization of this cysteine proteinase inhibitor in tomato leaf blade. Methyl jasmonate treated wild type plants showed accumulation of crystalline structures that were specifically and strongly stained with polyclonal antibodies against tomato cystatin. Crystalline cystatin was found in palisade and spongy parenchyma cells and immuno-gold electron microscopy analysis revealed that the crystals were compartmentalized in the cytosol. The same pattern in localization of cystatin was observed in transgenic tomato plants superexpressing prosystemin transgene. Our data showing the accumulation of cystatin in response to methyl jasmonate and in response to a overproduction of a wound signal corroborate the protective role of this inhibitor. 相似文献
1000.
利用微卫星标记分析七品种(类群)小型猪的遗传多样性 总被引:9,自引:0,他引:9
采用世界粮农组织(FAO)和国际动物遗传学会(ISAG)推荐的27个微卫星,对久仰香猪、剑白香猪、从江香猪、环江香猪、黑香猪(贵州省种猪场)、五指山猪和滇南小耳猪等我国7小型猪种(类群)及杜洛克、长白和大白等3个外来猪种的群内遗传变异性和群间遗传差异进行了分析。结果表明,7个小型猪品种(类群)均有较高的群内遗传变异,但久仰、剑白、从江和环江4个香猪类群的群内遗传变异(平均多态信息含量(PIC)为0.61~0.64)显著低于其他3个小型猪种(平均PIC为0.80~0.84)。久仰、剑白、从江和环江4个香猪类群彼此间的遗传差异较小(奈氏标准遗传距离为0.12~0.22),但它们与其他3个小型猪种有较大的遗传差异(奈氏标准遗传距离为1.61~1.96),与3个外来猪种的遗传差异更大(奈氏标准遗传距离为1.99~3.30)。通过聚类分析,可将这些猪种清晰地分为3大类,久仰、剑白、从江和环江4个香猪类群紧密地聚为一类,其他3个小型猪种聚为一类,3个外来猪种聚为另一类。这一结果基本符合这些猪种的地理分布和品种来源。
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