棉花耐盐变异体的遗传分析

利用花粉管通道技术将耐起因三的罗布麻（Ａｐｏｃｙｎｕｍ ｖｅｎｅｔｕｍ）ＤＮＡ导入鲁棉６号,从后代中筛选出棉花耐盐变异体山农０１１。对山农０１１及其杂种后代进行了抗盐遗传和基因效应分析。结果表明：变异体的耐盐性是由核基因控制的。其遗传基因效应以加性效应为主,并存在较大的加快互作效应。该耐盐变异体可作为一良好的抗盐种质材料利用。     

油松优树选择方法的研究

论述了优树选择应遵循优良种源区选择优良林分,优良林分中的选择优良单株的系统选择原则。提出了标准差法选择方法和标准,要求优树胸径大于林分平均胸径加２倍标准差,且优树年平均出长量：胸径大于０．９ｃｍ、树高大于０．５ｍ。根据１９株人工林优树周转的标准地每木实测数据,对小标准地法和大树选优法作了比较;根据３２株优树的选优结果,比较了１－５株大树法选优法之间的相关关系,大树法间相关都显著,但以３株法为最好;     

Cr^6＋对莼菜冬芽叶片急性毒害与保护酶系活性变化关系的研究

研究了Ｃｒ＾６＋的急性毒害对莼菜冬芽叶片的伤害程度和可溶性蛋白质、ＳＯＤ、ＣＡＴ、ＰＯＤ活性和ＭＤＡ含量变化之间的关系。在以０．５ｍｍｏｌ／Ｌ￣２．０ｍｍｏｌ／Ｌ浓度的Ｃｒ＾６＋处理时,冬芽叶片受害的程序明显地与处理浓度和处理时间呈正相关;可溶性蛋白质只在处理４ｄ时出现较大变化,其含量随处理浓度的提高而急剧增加;随着处理浓度的增加,ＳＯＤ、ＣＡＴ和ＰＯＤ的活性峰出现的时间不断后推,并且ＰＯＤ的活性     

抗氰交替途径参与马铃薯软腐病菌对马铃薯块茎的侵染

马铃薯软腐病菌中的强致病菌Ｅｃｃ、弱致病菌Ｅｃｃｂ在马铃薯块茎中诱导抗氰交替途径运行的能力依次为Ｅｃｃ、Ｅｃｃｂ。即病原菌的致病性越强,诱导抗氰交替途径的能力越强,显示病原菌对寄主植物的侵染过程与寄主植物体内抗氰交替途径的运行密切相关。ＳＨＡＭ抑制剂试验表明病原菌对寄主植物的侵染过程中寄主植物中抗氰交替途径的运行可增强寄主植物的感病性。信号传导试验显示ＬａＣｌ３、ＬｉＣｌ可通过促进体内抗氰交替途径     

蝗总科染色体研究及科级综合比较（直翅目）

对国际范围内蝗总科染色体研究简史及细胞分类学研究概况进行了回顾,并对我国蝗总科细胞分类学研究进展作了简要介绍。在此基础上,本文根据染色体数目、形态、染色体分组型式、性染色体位置、Ｃ带结构特征及异染色质总含量值等多方面特征对蝗总科８个科细胞分类学相互关系进行了综合比较。结果表明：癞蝗科染色体特点明显,进化位置独特;锥头蝗科与瘤锥蝗科相互近缘;斑腿蝗科与斑翅蝗科较为相近,网翅蝗科与槌角蝗科在染色体多项     

计算机在昆虫分类中的新用途

计算机作为一种昆虫分类的工具 ,已广泛用于昆虫的数值分类和支序分类中。随着多媒体计算机以及计算机网络技术的迅速发展 ,它在昆虫分类中又有了新的用途。多媒体计算机的使用将使传统的形态分类产生质的飞跃 ,并能通过互联网核对模式标本和进行昆虫种类的共同鉴定 ,使传统分类充满新的活力。     

一步多重PCR同时检测甲型肝炎和脊髓灰质炎病毒研究

建立的一步ＰＣＲ方法即反转录和ＰＣＲ在同一管中进行,同时检测甲型肝炎和脊髓灰质炎病毒病毒ＲＮＡ。实验中对不同的反转录温度以及一步多重ＰＣＲ的特异性和灵敏度进行了探讨。结果表明：４２℃、５０℃反转录时ｐｏｌｉｏ有非特异性条带出现,６０℃反转录特异性较好,而ＨＡＶ在三种不同的反转录温度下均得到牧场划性较好的条带;应用一步ＰＣＲ同时检测两种病毒与检测单一病毒的灵敏度基本一致,但在同等反应条件下后者的反应效率高于前者,特别是在检测ＨＡＶ时。     

乙肝孕妇肝功能指标变化与早产相关性的临床分析

目的:探讨乙型肝炎病毒感染伴肝功能异常肝功能指标变化对孕妇早产发生的影响.方法:选择2007年1月-2008年12月间在我院产检及住院分娩的156例孕28周至37周存在肝功异常的乙肝孕妇进行了回顾性分析.结果:晚期妊娠合并病毒性乙型肝炎患者早产组与非早产组血清转氨酶水平比较,差异有统计学意义(P＜0.05);两组总胆红素、总胆汁酸水平比较,差异显著(P＜0.01).孕晚期ALT轻度、中度以上异常,旱产发生率分别32%、90%,OR分别为2.7、51;AST轻度、中度以上异常,早产发生率分别35%、87%,OR分别为3.1、37.9;总胆汁酸轻度、中度以上异常,早产发生率分别39%、91%,OR分别为3.6、57.2;总胆红素轻度、中度以上异常,早产发生率分别49%、88%,OR分别为5.4、41.6;早产组与非早产组比较,羊水粪染及胎儿宫内窘迫差异有统计学意义(P＜0.01),剖宫产率、产后出血、新生儿窒息两组间差异无显著性(P＞0.05).结论:乙肝孕妇晚期妊娠血清ALT和AST、TBA、TBI1水平均与早产发生有关.     

Host sex‐specific parasites in a functionally dioecious fig: a preference way of adaptation to their hosts

Host–parasites interaction is a common phenomenon in nature. Diffusive coevolution might maintain stable cooperation in a fig–fig wasps system, in which the exploiter might diversify their genotype, phenotype, or behavior as a result of competition with pollinator, whereas the figs change flower syconia, fruits thickness, and syconia structure. In functionally dioecious Ficus auriculata, male figs and female figs contain two types of florets on separate plant, and share high similarities in outside morphology. Apocryptophagus (Sycophaginae, Chalcidoidea, Hymenoptera) is one of few groups of nonpollinating fig wasps that can reproduce within both male and female figs. On the basis of the morphology and DNA barcoding, evidence from partial sequences of mitochondrial cytochrome c oxidase I and nuclear internal transcribed spacer 2, we found that there are two nonsibling Apocryptophagus species living on male and female F. auriculata figs, respectively. We estimated that these two species diverged about 19.2 million years ago. Our study suggests that the host shift from Ficus variegate or Ficus prostrata fig species to male figs is a preference way for Apocryptophagus wasps to adapt to the separation of sexual function in diecious figs. Furthermore, to escape the disadvantage or sanction impact of the host, the exploiter Apocryptophagus wasps can preferably adapt to exploiting each sex of the figs, by changing their oviposition, niche shift, and habitat.     

Minimal physiologically-based pharmacokinetic modeling of DSTA4637A,A novel THIOMAB™ antibody antibiotic conjugate against Staphylococcus aureus,in a mouse model

DSTA4637A, a THIOMAB? antibody-antibiotic conjugate targeting Staphylococcus aureus, has shown promising bactericidal activity in a mouse model. DSTA4637A consists of a monoclonal anti-S. aureus antibody with an average of two rifalogue antibiotic molecules, dmDNA31, linked to its light chains. The goal of this study was to develop a minimal physiologically-based pharmacokinetic (mPBPK) model to characterize the pharmacokinetic (PK) properties of three analytes of DSTA4637A (i.e., total antibody, antibody-conjugated dmDNA31, and unconjugated dmDNA31) in mice, and to predict pharmacokinetics of DSTA4637A analytes in humans, as well as to provide an initial assessment for potential PK drug-drug interactions (DDI) in clinical trials via cross-species scaling of the mPBPK model. In the proposed model, selected organs, including heart, liver, and kidney, were connected anatomically with plasma and lymph flows. Mouse plasma and tissue concentrations of the three analytes of DSTA4637A were fitted simultaneously to estimate the PK parameters. Cross-species scaling of the model was performed by integrating allometric scaling and human physiological parameters. The final mPBPK model was able to successfully capture PK profiles of three DSTA4637A analytes in mouse plasma and in investigated organs. The model predicted a steady-state peak unbound dmDNA31 concentration lower than 5% of the IC₅₀ of dmDNA31 towards cytochrome P450 following 100 mg/kg weekly intravenous dose, which suggests a low risk of PK DDI in humans for DSTA4637A with co-administered cytochrome P450 substrates. The proposed mPBPK modeling and cross-species scaling approaches provide valuable tools that facilitate the understanding and translation of DSTA4637A disposition from preclinical species to humans.