全文获取类型
收费全文 | 40392篇 |
免费 | 4355篇 |
国内免费 | 12篇 |
专业分类
44759篇 |
出版年
2022年 | 301篇 |
2021年 | 528篇 |
2020年 | 344篇 |
2019年 | 421篇 |
2018年 | 547篇 |
2017年 | 513篇 |
2016年 | 895篇 |
2015年 | 1615篇 |
2014年 | 1569篇 |
2013年 | 2088篇 |
2012年 | 2363篇 |
2011年 | 2083篇 |
2010年 | 1451篇 |
2009年 | 1320篇 |
2008年 | 1815篇 |
2007年 | 1791篇 |
2006年 | 1605篇 |
2005年 | 1563篇 |
2004年 | 1472篇 |
2003年 | 1269篇 |
2002年 | 1265篇 |
2001年 | 1230篇 |
2000年 | 1233篇 |
1999年 | 1106篇 |
1998年 | 610篇 |
1997年 | 562篇 |
1996年 | 555篇 |
1995年 | 539篇 |
1994年 | 491篇 |
1993年 | 512篇 |
1992年 | 1007篇 |
1991年 | 758篇 |
1990年 | 775篇 |
1989年 | 751篇 |
1988年 | 654篇 |
1987年 | 596篇 |
1986年 | 611篇 |
1985年 | 708篇 |
1984年 | 533篇 |
1983年 | 413篇 |
1982年 | 344篇 |
1981年 | 313篇 |
1980年 | 253篇 |
1979年 | 378篇 |
1978年 | 344篇 |
1977年 | 244篇 |
1976年 | 228篇 |
1975年 | 197篇 |
1974年 | 289篇 |
1973年 | 251篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
991.
Krook J; Vreugdenhil D; Dijkema C; van der Plas L 《Journal of experimental botany》1998,49(329):1917-1924
Cells were grown in batch culture on a mixture of 50 mM glucose and
fructose as the carbon source; either the glucose or the fructose was
[1-13C]-labelled. In order to investigate the uptake
and conversion of glucose and fructose during long-term labelling
experiments in cell suspensions of Daucus carota L.,
samples were taken every 2 d during a 2 week culture period and sucrose and
starch were assayed by means of HPLC and 13C-nuclear
magnetic resonance. The fructose moieties of sucrose had a lower labelling
percentage than the glucose moieties. Oxidative pentose phosphate pathway
activity in the cytosol is suggested to be responsible for this loss of
label of especially C-1 carbons. A combination of oxidative pentose
phosphate pathway activity, a relatively high activity of pathway to
sucrose synthesis and a slow equilibration between glucose-6-phosphate and
fructose-6-phosphate could explain these results. Starch contained glucose
units with a much lower labelling percentage than glucose moieties of
sucrose: it was concluded that a second, plastid-localized, oxidative
pentose phosphate pathway was responsible for removal of C-1 carbons of the
glucosyl units used for synthesis of starch. Redistribution of label from
[1-13C]-hexoses to
[6-13C]-hexoses also occurred: 18-45% of the label
was found at the C-6 carbons. This is a consequence of cycling between
hexose phosphates and those phosphates in the cytosol catalysed by PFP. The
results indicate that independent (oxidative pentose phosphate pathway
mediated) sugar converting cycles exist in the cytosol and
plastid.Key words: Daucus carotaL., cell suspensions,
carbon-13 nuclear magnetic resonance, 13C-NMR,
carbohydrate cycling, oxidative pentose phosphate pathway, plastid.
相似文献
992.
L. Barton Browne A. C. M. van Gerwen W. G. Vogt 《Entomologia Experimentalis et Applicata》1990,55(1):33-40
Oviposition by Lucilia cuprina Wiedemann (Diptera, Calliphoridae) was examined in relation to period of oviposition site-deprivation and egg-load. Effects of oviposition site-deprivation were examined by comparing oviposition performance of individual females that had matured their batch of oocytes within the previous 24 h with that of females which had reached ovarian maturity 8 days previously. Egg-load was manipulated by causing females of this anautogenous species to consume different amounts of protein-rich material. In no-choice experiments, individual females of the different categories were given access for 4 h to oviposition substrate, soaked with (i) liver exudate, (ii) the exudate diluted 16-fold or (iii) the undilated exudate containing the oviposition deterrent sodium chloride at a concentration of 2 M. These solutions elicited oviposition from different proportions of females, but neither these proportions, nor the interval between introduction of the oviposition site and the initiation of oviposition, was significantly affected by the period of oviposition site-deprivation or the number of eggs matured by the females.
Résumé L'effet de la privation de lieu de ponte a été étudié en comparant les pontes de femelles isolées ayant formé leurs ufs mûrs dans les 24 heures précédentes, à celles de femelles ayant atteint leur maturité sexuelle 8 jours avant. La rétention ovocytaire est provoquée en faisant consommer aux femelles de cette espèce anautogène différentes quantités d'aliments riches en protéines. La ponte de femelles dont le contingent total de leurs ovocytes s'est développé, — c'est-à-dire 260 —, après consommation ad libitum de foie de mouton pendant 48 heures, a été comparée à celle de femelles ayant formé 190 ovocytes mûrs après ingestion d'une quantité limitée de jus de foie.Dans des expériences sans choix, les femelles isolées de différences catégories ont eu accès pendant 4 heures au substrat de ponte trempé: 1) dans du jus de foie, 2) dans du jus dilué 16 fois, 3) dans du jus de foie non dilué mais contenant NaCl (inhibiteur de la ponte) à la concentration de 2 M. Le jus non dilué a provoqué une forte stimulation, induisant la ponte de 80% des femelles. Le jus dilué et celui contenant NaCl n'ont induit la ponte que de 40% des femelles avec des niveaux de stimulation bien plus faibles. La date d'introduction du lieu de ponte et le taux de rétention des ovocytes mûrs n'ont eu auçun effet sur la proportion de femelles réagissant à ces 3 types de stimulation.相似文献
993.
We have recently described an insulin-resistant patient with leprechaunism (leprechaun G.) having a homozygous leucine----proline mutation at amino acid position 233 in the alpha-chain of the insulin receptor. The mutation results in a loss of insulin binding to cultured fibroblasts. Fibroblasts from the patient and control individuals were used to quantify the stimulation of 2-deoxyglucose uptake by insulin and insulin-like growth factor 1 (IGF-1). Insulin hardly stimulates basal 2-deoxyglucose uptake in the patient's fibroblasts whereas in control fibroblasts the uptake of 2-deoxyglucose is stimulated by insulin approximately 1.7 times. In contrast, IGF-1 stimulates hexose uptake in the patient's fibroblasts 1.8 times, a similar value to that obtained by stimulation of control fibroblasts with insulin or IGF-1. With both types of fibroblasts, maximal IGF-1 response is reached at about 10 nM IGF-1, the ED50 being approximately 4 nM. The results indicate that the insulin responsive glucose transport in primary fibroblasts is functionally linked to the receptor for IGF-1. Insulin binds with an approximately 200-fold lower affinity to IGF-1 receptors, compared to homologous IGF-1 binding. As an insulin concentration of 10 microM is unable to give maximal stimulation of glucose uptake in the patient's fibroblasts, which is already seen with 10 nM IGF-1, it seems that occupation of IGF-1 receptors by insulin on the patient's cells is less efficient at stimulating hexose uptake compared to homologous activation. 相似文献
994.
M. Koornneef T. D. G. Bosma C. J. Hanhart J. H. van der Veen J. A. D. Zeevaart 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1990,80(6):852-857
Summary In tomato, nine independent EMS-induced mutants representing recessive mutations at three different loci (gib-1, gib-2, and gib-3) were isolated. Six of these have an almost absolute gibberellin requirement for seed germination and elongation growth. In addition, the leaves are darker green, smaller, and changed in structure as compared to wild type. The three other mutants, which germinate without GA, are allelic to specific, nongerminating mutants and have less severe mutant characteristics. The respective loci are situated on three different chromosomes. The genes identified by these mutants control steps in gibberellin biosynthesis, as endogenous gibberellins are strongly reduced. 相似文献
995.
William?H.?Morgan Elisa?Thébault Colleen?L.?Seymour F.?J.?Frank?van?VeenEmail author 《BioControl》2017,62(2):175-184
Host–parasitoid dynamics are intrinsically unstable unless the risk of parasitism is sufficiently heterogeneous among hosts. Spatial aggregation of parasitoids can contribute to this heterogeneity, stabilising host–parasitoid population dynamics and thereby reducing pest outbreaks. We examined the spatial distribution of mango gall fly (Procontarinia matteiana, Kiefer and Cecconi), a non-native pest of South African mango orchards, which is controlled by a single parasitoid (Chrysonotomyia pulcherrima, Kerrich). We assessed whether spatial aggregation of parasitoids is associated with proximity to natural vegetation and/or to host density-dependent and host density-independent factors at three spatial scales. We found evidence for higher parasitism rates near natural vegetation at the field scale, and inverse host-density dependent and density-independent parasitoid aggregation at both the leaf scale and field scale. Therefore, we conclude that natural vegetation plays a role in promoting stabilising aggregation of parasitoids, possibly through provision of non-host resources (nectar, pollen), in this system. 相似文献
996.
Leela J. Chakravarti Victor H. Beltran Madeleine J. H. van Oppen 《Global Change Biology》2017,23(11):4675-4688
Climate warming is occurring at a rate not experienced by life on Earth for 10 s of millions of years, and it is unknown whether the coral‐dinoflagellate (Symbiodinium spp.) symbiosis can evolve fast enough to ensure coral reef persistence. Coral thermal tolerance is partly dependent on the Symbiodinium hosted. Therefore, directed laboratory evolution in Symbiodinium has been proposed as a strategy to enhance coral holobiont thermal tolerance. Using a reciprocal transplant design, we show that the upper temperature tolerance and temperature tolerance range of Symbiodinium C1 increased after ~80 asexual generations (2.5 years) of laboratory thermal selection. Relative to wild‐type cells, selected cells showed superior photophysiological performance and growth rate at 31°C in vitro, and performed no worse at 27°C; they also had lower levels of extracellular reactive oxygen species (exROS). In contrast, wild‐type cells were unable to photosynthesise or grow at 31°C and produced up to 17 times more exROS. In symbiosis, the increased thermal tolerance acquired ex hospite was less apparent. In recruits of two of three species tested, those harbouring selected cells showed no difference in growth between the 27 and 31°C treatments, and a trend of positive growth at both temperatures. Recruits that were inoculated with wild‐type cells, however, showed a significant difference in growth rates between the 27 and 31°C treatments, with a negative growth trend at 31°C. There were no significant differences in the rate and severity of bleaching in coral recruits harbouring wild‐type or selected cells. Our findings highlight the need for additional Symbiodinium genotypes to be tested with this assisted evolution approach. Deciphering the genetic basis of enhanced thermal tolerance in Symbiodinium and the cause behind its limited transference to the coral holobiont in this genotype of Symbiodinium C1 are important next steps for developing methods that aim to increase coral bleaching tolerance. 相似文献
997.
B K Gillard D Blanchard J F Bouhours J P Cartron J A van Kuik J P Kamerling J F Vliegenthart D M Marcus 《Biochemistry》1988,27(13):4601-4606
The Cad antigen is a rare erythrocyte blood group antigen expressed on both sialoglycoprotein and ganglioside structures. It is related both serologically and biochemically to the Sda blood group antigen expressed on over 90% of Caucasian erythrocytes. We reported previously that Cad erythrocytes contain a novel ganglioside that binds Helix pomatia lectin and inhibits human anti-Sda antibody. We have now purified the Cad ganglioside and determined its structure. The ganglioside contained Glc-Gal-GlcNAc-GalNAc-NeuAc in a molar ratio of 1.00:1.94:0.95:0.93:1.05. Its chromatographic mobility was between that of GM1 and GD3. After treatment with beta-hexosaminidase (human placenta Hex A), the product migrated with 2-3-sialosylparagloboside (IV3NeuAcnLc4OseCer), it no longer bound H. pomatia lectin, and it acquired the ability to bind an antibody to sialosylparagloboside. Treatment of this material with neuraminidase (Vibrio cholerae) yielded a product with the mobility of paragloboside (nLc4OseCer) that bound monoclonal antibody 1B2, which is specific for terminal N-acetyllactosaminyl structures. Treatment of the Cad ganglioside with Arthrobacter ureafaciens neuraminidase yielded a product reactive with monoclonal antibody 2D4, which is specific for terminal GalNAc beta (1-4)Gal structures. These data provide strong evidence that the Cad ganglioside structure is GalNAc beta (1-4)[NeuAc alpha (2-3)]Gal beta (1-3)Gal beta (1-4)GlcCer. 1H NMR analysis also supports the conclusion that the terminal GalNAc is linked beta (1-4) to Gal. High-performance thin-layer chromatographic ganglioside patterns from three blood group Cad individuals showed a direct correlation between the quantity of Cad ganglioside and the strength of Cad antigen expression on the erythrocytes, as measured by hemagglutination.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
998.
NIKOLAAS J. van der MERWE JULIA A. LEE THORP RICHARD H. V. BELL† 《African Journal of Ecology》1988,26(2):163-172
Stable carbon isotope ratios have been successfully used to assess modern animal diets and to reconstruct prehistoric diets of animals and humans (Vogel & van der Merwe, 1977; van der Merwe & Vogel, 1978; Burleigh & Brothwell, 1978; Vogel, 1978a; DeNiro & Epstein, 1978; Tieszen et al., 1979; Tieszen & Imbamba, 1980; Chisholm, Nelson & Schwarcz, 1982; Tauber, 1981). We have used 13C/12C ratio measurements of bone collagen to study the diets of African elephants in twelve wildlife refuges. These represent most of the habitats in which elephants live, including such diverse plant communities as primary rain forest, savanna woodland and desert. The δ13C values were found to have a simple linear relationship with tree density in most cases. When translated into relative amounts of dietary browse (C3 plants) and graze (C4 plants), the grass content is seen to be systematically under-represented, presumably due to inefficient metabolism. This does not affect the relationship between elephant diet and tree density, which has implications for the study of elephant-woodland interactions, and for reconstructions of past African environments. 相似文献
999.
Summary The genusPlebeia has a special significance for the study of social evolution of stingless bees: morphologically primitive, its species display a wealth of behavioural evolution, especially with respect to the oviposition process. We comparePlebeia remota with the few other members of the genus studied so far.Related to its subtropical geographical range, brood production is seasonal (there is no brood in the colony in colder months), and adult workers occur as summer and winter bees. The nest is in tree cavities, and the involucrum is absent or restricted to the winter period. Brood cells are arranged in horizontal combs, and new cells are built completely synchronously. Each series consists of up to 50 cells, their number being mainly dependent on colony size. Construction speed is remarkably constant, allowing 4–6 batches per 24 hours. Cell building and provisioning are activities of a small group of specialized workers.The oviposition cycle follows the classical subdivisions for stingless bees. During the patrolling phase a worker may offer a trophic egg in a most remarkable way: while retreating backward from the queen she bends the abdomen under thorax and head, and lays an egg on the comb. This egg is eaten by the queen or a worker.The provisioning, oviposition and operculation of all cells occur simultaneously, each cell is provisioned by 4–9 workers. Localization of a cell by the queen may be facilitated by its characteristic guard, which defends the cell against the approaching queen. The degree of synchronization within a batch is very high: the duration per cell lasts 420–950 sec, the batch of up till 50 cells needs only 557–1160 sec. Operculation is done by a worker that was not involved in the previous steps.Males are generally produced by the queen. Several male producing cycles per year occur. In orphan colonies laying workers give rise to males, and in queenright colonies workers may occasionally reproduce as well.Division of labour follows the general pattern for stingless bees; however, cell building and provisioning are activities of a specialized group of workers. 相似文献
1000.