全文获取类型
收费全文 | 5513篇 |
免费 | 287篇 |
国内免费 | 259篇 |
出版年
2022年 | 36篇 |
2021年 | 62篇 |
2020年 | 18篇 |
2019年 | 13篇 |
2018年 | 91篇 |
2017年 | 60篇 |
2016年 | 133篇 |
2015年 | 276篇 |
2014年 | 273篇 |
2013年 | 299篇 |
2012年 | 377篇 |
2011年 | 624篇 |
2010年 | 426篇 |
2009年 | 183篇 |
2008年 | 197篇 |
2007年 | 158篇 |
2006年 | 105篇 |
2005年 | 302篇 |
2004年 | 474篇 |
2003年 | 292篇 |
2002年 | 180篇 |
2001年 | 166篇 |
2000年 | 86篇 |
1999年 | 89篇 |
1998年 | 68篇 |
1997年 | 89篇 |
1996年 | 45篇 |
1995年 | 45篇 |
1994年 | 28篇 |
1993年 | 120篇 |
1992年 | 99篇 |
1991年 | 59篇 |
1990年 | 62篇 |
1989年 | 58篇 |
1988年 | 48篇 |
1987年 | 29篇 |
1986年 | 28篇 |
1985年 | 42篇 |
1983年 | 10篇 |
1982年 | 14篇 |
1981年 | 12篇 |
1980年 | 10篇 |
1979年 | 20篇 |
1978年 | 12篇 |
1977年 | 10篇 |
1974年 | 14篇 |
1973年 | 10篇 |
1972年 | 10篇 |
1963年 | 11篇 |
1962年 | 14篇 |
排序方式: 共有6059条查询结果,搜索用时 13 毫秒
111.
Donald J. Cummings Léon Belcour Claude Grandchamp 《Molecular & general genetics : MGG》1979,171(3):239-250
Summary Mitochondrial (Mt) DNA from mitochondrial mutants of race s Podospora anserina and from senescent cultures of races s and A was examined. In mutants, we observed that fewer full length circles (31 ) were present; instead, smaller circles characteristic for each mutant sudied were found. Eco Rl digestion of these mutant MtDNAs indicated that in certain mutants, although specific fragments were absent, the total molecular weight of the fragments was not much different than wild-type.The properties of senescent MtDNA was strikingly different from either wild-type or mutant Mt DNA. First, a multimeric set of circular DNA was observed for both race s and A, with a monomeric repeat size of 0.89 . These circles ranged in size from 0.89 to greater than 20 ; only one molecule out of some 200 molecules was thought to be of full length (31 ). Density gradient analysis showed that there were two density species: a majority were at the same density as wild-type (1.694 g/cm3) and a second at 1.699 g/cm3. Most of the circular molecules from MtDNA isolated by either total DNA extraction or by extraction of DNA from isolated mitochondria were contained in the heavy DNA fraction. Eco R1 enzymatic digestion indicated that the light DNA had several fragments (amounting to about 23×106 daltons) missing, compared with young, wild-type MtDNA. Heavy senescent MtDNA was not cleaved by Eco R1. Analysis with Hae III restriction endonuclease showed also that light senescent MtDNA was missing certain fragments. Heavy MtDNA of average size 20×106 daltons, yielded only one fragment, 2,500 bp long, by digestion with Hae III restriction endonuclease. Digestion of heavy DNA with Alu I enzyme yielded 10 fragments totalling 2,570 bp. By three criteria, electron-microscopy, Eco R1 and Hae digestion, we conclude that the heavy MtDNA isolated from senescent cultures of Podospora anserina consisted of a monomeric tandemly repeating subunit of about 2,600 bp length.These results on the properties of senescent MtDNA are discussed with regard to the published properties of the rho
- mutation in the yeast, S. cerevisiae. 相似文献
112.
113.
木姜子叶精油的化学成分研究 总被引:2,自引:0,他引:2
采用气相色谱-质谱联用仪对产于秦岭太白山的木姜子叶的挥发油成分进行分析鉴定,从分离出的79个峰中鉴定了32种成分,其含量占挥发油总量的90.59%,主要成分为1,3,3-三甲基-乙-氧杂双环[2,2,2]辛烷(59.96%),1,8-桉油醇(8.96%),香茅醛(6.86%),2-甲基-5-(1-甲基乙烯基)环己酮(4.34%)和澄花醇乙酯(3.19%)。 相似文献
114.
寻螨属Zetzellia在中国曾报道了两种:苹果寻螨Z.mail(Ewing)和北京寻螨Z.beijing Wang and Xu1987.本文描述了寻螨属一新种,庐山寻螨Z.lushanensis sp.nov.模式标本保存于江西大学生物系.文内测量单位为μm。 相似文献
115.
急性病毒性肝炎的发病率慢性肝炎患病率和肝病死亡率的研究 总被引:15,自引:0,他引:15
1984~1987年,在黑龙江、河北、河南、湖南、上海五个省市城乡10.08855人口中进行急性肝炎发病率、慢性肝炎患病率、与病毒性肝炎有关的肝病死亡率的研究。急性肝炎标化发病率为152.19/10万,主要发生在20~50岁组人群;因无甲肝暴发流行,除上海外各点季节发病率分布均衡。慢性肝炎标化患病率为158.25/10万(诊断标准为6个月前有明确急性肝炎病史,现有明显的临床症状或体征,肝功能异常,故实际慢肝患病率要高于此数字);与病毒性肝炎有关的肝病死亡(包括肝癌)标化率为22.65/10万,其中肝病为 13.14/10万。男性死亡率显著高于女性。 相似文献
116.
European Committee for Clinical Laboratory Standards Subcommittee on Reference Materials for Tissue Stains 《The Histochemical journal》1992,24(4):233-235
The names and affiliations of the SRMTS members are cited in Part I: terminology and general principles,Histochem. J. (1992)24, 217–19. 相似文献
117.
Two distinct members of the ADP-ribosylation factor family of GTP-binding proteins regulate cell-free intra-Golgi transport. 总被引:14,自引:0,他引:14
We have used an intra-Golgi transport assay to identify GTP-binding proteins involved in regulation of protein traffic. Two soluble proteins of 20 kd were purified by their ability to mediate GTP gamma S-dependent inhibition of transport. These GTP-dependent Golgi binding factors, or GGBFs, exhibit a 3-fold difference in activity and are differentiated by their hydrophobicity, isoelectric points, and apparent size. Removal of 80% of GGBFs from cytosol abolishes GTP gamma S sensitivity but does not affect inhibition by aluminum fluoride. We demonstrate that GGBFs are members of the ADP-ribosylation factor (ARF) family. Recombinant ARF1 exhibits GGBF activity and myristoylation is required. The distinct biochemical properties of GGBFs indicate that members of the ARF family may have related but distinct functions in intracellular transport. 相似文献
118.
119.
A Skogl?f I Nilsson S Gustafsson J Deinum P O G?the 《Biochimica et biophysica acta》1990,1041(1):22-30
The angiotensin I-converting enzyme (peptidyl-dipeptide hydrolase, EC 3.4.15.1) inhibitor, ramiprilat (2-[N-[(S)-1-ethoxycarbonyl-3-phenylpropyl]-L-Ala]-(1S,3S,5S)-2- azabicyclo[3.3.0]octane-3-carboxylic acid), is shown to exist in tow conformational isomers, cis and trans, which interconvert around the amide bond. The two conformers were separated by reversed-phase high-performance liquid chromatography. The conformers were identified by nuclear Overhauser effect measurements. From line shape analysis the isomerization rate constants were determined to be kcis----trans = 15 s-1 and ktrans----cis = 5 s-1 at 368 K in [2H]phosphate buffer (p2H 7.5). By enzyme kinetic studies using 3-(2-furylacryloyl)-L-Phe-Gly-Gly as substrate, the trans conformer was found to be the most potent enzyme inhibitor, whereas the cis conformer had a very low inhibitory effect. A new inhibition mechanism is presented for this type of slow, tight-binding inhibitors that contain an amide bond. This mechanism involves an equilibrium between the two conformers and the enzyme-bound inhibitor complex. 相似文献
120.
The epididymis of adult rams is the primary source of alpha-glucosidase in seminal plasma. Two breeds of rams were selected to ascertain whether the enzyme was under androgenic control during adult life of rams. Opposite variations between serum testosterone and alpha-glucosidase were recorded over a period of 16 months in Suffolk and Finnish Landrace. In addition, the highest percentage of sperm motility was associated with a low alpha-glucosidase content of seminal plasma. Data from this study suggest that seasonal variations of testosterone in adult rams exert a negative control on the presence of alpha-glucosidase in semen. 相似文献