全文获取类型
收费全文 | 221篇 |
免费 | 18篇 |
出版年
2021年 | 3篇 |
2020年 | 4篇 |
2018年 | 2篇 |
2017年 | 2篇 |
2016年 | 5篇 |
2015年 | 4篇 |
2014年 | 7篇 |
2013年 | 5篇 |
2012年 | 12篇 |
2011年 | 10篇 |
2010年 | 9篇 |
2009年 | 5篇 |
2008年 | 13篇 |
2007年 | 5篇 |
2006年 | 7篇 |
2005年 | 5篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 15篇 |
2001年 | 10篇 |
2000年 | 6篇 |
1999年 | 11篇 |
1997年 | 3篇 |
1996年 | 4篇 |
1994年 | 2篇 |
1993年 | 3篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 2篇 |
1983年 | 2篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1978年 | 2篇 |
1977年 | 1篇 |
1976年 | 4篇 |
1975年 | 6篇 |
1973年 | 4篇 |
1972年 | 1篇 |
1971年 | 5篇 |
1970年 | 6篇 |
1969年 | 2篇 |
1968年 | 3篇 |
1967年 | 6篇 |
1965年 | 1篇 |
1962年 | 1篇 |
1957年 | 1篇 |
1954年 | 1篇 |
排序方式: 共有239条查询结果,搜索用时 15 毫秒
211.
212.
213.
Felix Beulig Verena B Heuer Denise M Akob Bernhard Viehweger Marcus Elvert Martina Herrmann Kai-Uwe Hinrichs Kirsten Küsel 《The ISME journal》2015,9(3):746-759
Effects of extremely high carbon dioxide (CO2) concentrations on soil microbial communities and associated processes are largely unknown. We studied a wetland area affected by spots of subcrustal CO2
degassing (mofettes) with focus on anaerobic autotrophic methanogenesis and acetogenesis because the pore gas phase was largely hypoxic. Compared with a reference soil, the mofette was more acidic (ΔpH ∼0.8), strongly enriched in organic carbon (up to 10 times), and exhibited lower prokaryotic diversity. It was dominated by methanogens and subdivision 1
Acidobacteria, which likely thrived under stable hypoxia and acidic pH. Anoxic incubations revealed enhanced formation of acetate and methane (CH4) from hydrogen (H2) and CO2
consistent with elevated CH4
and acetate levels in the mofette soil.
13CO2
mofette soil incubations showed high label incorporations with ∼512 ng
13C g (dry weight (dw)) soil−1 d−1
into the bulk soil and up to 10.7 ng
13C g (dw) soil−1 d−1
into almost all analyzed bacterial lipids. Incorporation of CO2-derived carbon into archaeal lipids was much lower and restricted to the first 10 cm of the soil. DNA-SIP analysis revealed that acidophilic methanogens affiliated with
Methanoregulaceae
and hitherto unknown acetogens appeared to be involved in the chemolithoautotrophic utilization of
13CO2. Subdivision 1
Acidobacteriaceae
assimilated
13CO2
likely via anaplerotic reactions because
Acidobacteriaceae
are not known to harbor enzymatic pathways for autotrophic CO2
assimilation. We conclude that CO2-induced geochemical changes promoted anaerobic and acidophilic organisms and altered carbon turnover in affected soils. 相似文献
214.
215.
216.
pH Gradient-Induced Heterogeneity of Fe(III)-Reducing Microorganisms in Coal Mining-Associated Lake Sediments 下载免费PDF全文
Marco Blthe Denise M. Akob Joel E. Kostka Kathrin Gschel Harold L. Drake Kirsten Küsel 《Applied microbiology》2008,74(4):1019-1029
Lakes formed because of coal mining are characterized by low pH and high concentrations of Fe(II) and sulfate. The anoxic sediment is often separated into an upper acidic zone (pH 3; zone I) with large amounts of reactive iron and a deeper slightly acidic zone (pH 5.5; zone III) with smaller amounts of iron. In this study, the impact of pH on the Fe(III)-reducing activities in both of these sediment zones was investigated, and molecular analyses that elucidated the sediment microbial diversity were performed. Fe(II) was formed in zone I and III sediment microcosms at rates that were approximately 710 and 895 nmol cm−3 day−1, respectively. A shift to pH 5.3 conditions increased Fe(II) formation in zone I by a factor of 2. A shift to pH 3 conditions inhibited Fe(II) formation in zone III. Clone libraries revealed that the majority of the clones from both zones (approximately 44%) belonged to the Acidobacteria phylum. Since moderately acidophilic Acidobacteria species have the ability to oxidize Fe(II) and since Acidobacterium capsulatum reduced Fe oxides at pHs ranging from 2 to 5, this group appeared to be involved in the cycling of iron. PCR products specific for species related to Acidiphilium revealed that there were higher numbers of phylotypes related to cultured Acidiphilium or Acidisphaera species in zone III than in zone I. From the PCR products obtained for bioleaching-associated bacteria, only one phylotype with a level of similarity to Acidithiobacillus ferrooxidans of 99% was obtained. Using primer sets specific for Geobacteraceae, PCR products were obtained in higher DNA dilutions from zone III than from zone I. Phylogenetic analysis of clone libraries obtained from Fe(III)-reducing enrichment cultures grown at pH 5.5 revealed that the majority of clones were closely related to members of the Betaproteobacteria, primarily species of Thiomonas. Our results demonstrated that the upper acidic sediment was inhabited by acidophiles or moderate acidophiles which can also reduce Fe(III) under slightly acidic conditions. The majority of Fe(III) reducers inhabiting the slightly acidic sediment had only minor capacities to be active under acidic conditions. 相似文献
217.
K Küsel T Dorsch G Acker E Stackebrandt 《Applied and environmental microbiology》1999,65(8):3633-3640
To evaluate the microbial populations involved in the reduction of Fe(III) in an acidic, iron-rich sediment, the anaerobic flow of supplemental carbon and reductant was evaluated in sediment microcosms at the in situ temperature of 12 degrees C. Supplemental glucose and cellobiose stimulated the formation of Fe(II); 42 and 21% of the reducing equivalents that were theoretically obtained from glucose and cellobiose, respectively, were recovered in Fe(II). Likewise, supplemental H(2) was consumed by acidic sediments and yielded additional amounts of Fe(II) in a ratio of approximately 1:2. In contrast, supplemental lactate did not stimulate the formation of Fe(II). Supplemental acetate was not consumed and inhibited the formation of Fe(II). Most-probable-number estimates demonstrated that glucose-utilizing acidophilic Fe(III)-reducing bacteria approximated to 1% of the total direct counts of 4', 6-diamidino-2-phenylindole-stained bacteria. From the highest growth-positive dilution of the most-probable-number series at pH 2. 3 supplemented with glucose, an isolate, JF-5, that could dissimilate Fe(III) was obtained. JF-5 was an acidophilic, gram-negative, facultative anaerobe that completely oxidized the following substrates via the dissimilation of Fe(III): glucose, fructose, xylose, ethanol, glycerol, malate, glutamate, fumarate, citrate, succinate, and H(2). Growth and the reduction of Fe(III) did not occur in the presence of acetate. Cells of JF-5 grown under Fe(III)-reducing conditions formed blebs, i.e., protrusions that were still in contact with the cytoplasmic membrane. Analysis of the 16S rRNA gene sequence of JF-5 demonstrated that it was closely related to an Australian isolate of Acidiphilium cryptum (99.6% sequence similarity), an organism not previously shown to couple the complete oxidation of sugars to the reduction of Fe(III). These collective results indicate that the in situ reduction of Fe(III) in acidic sediments can be mediated by heterotrophic Acidiphilium species that are capable of coupling the reduction of Fe(III) to the complete oxidation of a large variety of substrates including glucose and H(2). 相似文献
218.
219.
220.
W Wehrli F Knüsel M Staehelin 《Biochemical and biophysical research communications》1968,32(2):284-288