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81.
Effects of an African weaver ant, Oecophylla longinoda, in controlling mango fruit flies (Diptera: Tephritidae) in Benin 总被引:1,自引:0,他引:1
Van Mele P Vayssières JF Van Tellingen E Vrolijks J 《Journal of economic entomology》2007,100(3):695-701
Six mango, Mangifera indica L., plantations around Parakou, northern Benin, were sampled at 2-wk intervals for fruit fly damage from early April to late May in 2005. Mean damage ranged from 1 to 24% with a weaver ant, Oecophylla longinoda (Latreille), being either abundant or absent. The fruit fly complex is made up of Ceratitis spp. and Bactrocera invadens Drew et al., a new invasive species in West Africa. In 2006, Ceratitis spp. peaked twice in the late dry season in early April and early May, whereas B. invadens populations quickly increased at the onset of the rains, from mid-May onward. Exclusion experiments conducted in 2006 with 'Eldon', 'Kent', and 'Gouverneur' confirmed that at high ant abundance levels, Oecophylla significantly reduced fruit fly infestation. Although fruit fly control methods are still at an experimental stage in this part of the world, farmers who tolerated weaver ants in their orchard were rewarded by significantly better fruit quality. Conservation biological control with predatory ants such as Oecophylla in high-value tree crops has great potential for African and Asian farmers. Implications for international research for development at the Consultative Group on International Agricultural Research level are discussed. 相似文献
82.
Génard M Bertin N Borel C Bussières P Gautier H Habib R Léchaudel M Lecomte A Lescourret F Lobit P Quilot B 《Journal of experimental botany》2007,58(5):917-928
The fruit is a hierarchically organized organ composed of cells from different tissues. Its quality, defined by traits such as fruit size and composition, is the result of a complex chain of biological processes. These processes involve exchanges (transpiration, respiration, photosynthesis, phloem and xylem fluxes, and ethylene emission) between the fruit and its environment (atmosphere or plant), tissue differentiation, and cell functioning (division, endoreduplication, expansion, metabolic transformations, and vacuolar storage). In order to progress in our understanding of quality development, it is necessary to analyse the fruit as a system, in which processes interact. In this case, a process-based modelling approach is particularly powerful. Such a modelling approach is proposed to develop a future 'virtual fruit' model. The value of a virtual fruit for agronomists and geneticists is also discussed. 相似文献
83.
Escherichia coli is the most common organism associated with asymptomatic bacteriuria (ABU) in humans. In contrast to uropathogenic E. coli (UPEC) that cause symptomatic urinary tract infection, very little is known about the mechanisms by which these strains colonize the urinary tract. Here, we have investigated the biofilm-forming capacity on abiotic surfaces of groups of ABU strains and UPEC strains in human urine. We found that there is a strong bias; ABU strains were significantly better biofilm formers than UPEC strains. Our data suggest that biofilm formation in urinary tract infectious E. coli seems to be associated with ABU strains and appears to be an important strategy used by these strains for persistence in this high-flow environment. 相似文献
84.
Kousik?Chandra Venkatesh?Ramakrishnan Yogendra?Sharma K.?V.?R.?CharyEmail author 《Journal of biological inorganic chemistry》2011,16(1):81-95
Neuronal calcium sensor-1 (NCS-1) interacts with many membranes and cytosolic proteins, both in a Ca2+-dependent and in a Ca2+-independent manner, and its physiological role is governed by its N-terminal myristoylation. To understand the role of myristoylation
in altering Ca2+ response and other basic biophysical properties, we have characterized the Ca2+ filling pathways in both myristoylated (myr) and non-myristoylated (non-myr) forms of NCS-1. We have observed that Ca2+ binds simultaneously to all three active EF-hands in non-myr NCS-1, whereas in the case of myr NCS-1, the process is sequential,
where the second EF-hand is filled first, followed by the third and fourth EF-hands. In the case of myr NCS-1, the observed
sequential Ca2+ binding process becomes more prominent in the presence of Mg2+. Besides, the analysis of 15N-relaxation data reveals that non-myr NCS-1 is more dynamic than myr NCS-1. The overall molecular tumbling correlation time
increases by approximately 20% upon myristoylation. Comparing the apo forms of non-myr NCS-1 and myr NCS-1, we found the possibility
of existence of some substates, which are structurally closer to the holo form of the protein. There are more such substates
in the case of non-myr NCS-1 than in the case of the myr NCS-1, suggesting that the former accesses larger volumes of conformational
substates compared with the latter. Further, the study reveals that the possibility of Ca2+ binding simultaneously to different parts of the protein is more favourable in non-myr NCS-1 than in myr NCS-1. 相似文献
85.
Imanishi L Vayssières A Franche C Bogusz D Wall L Svistoonoff S 《Molecular plant-microbe interactions : MPMI》2011,24(11):1317-1324
Among infection mechanisms leading to root nodule symbiosis, the intercellular infection pathway is probably the most ancestral but also one of the least characterized. Intercellular infection has been described in Discaria trinervis, an actinorhizal plant belonging to the Rosales order. To decipher the molecular mechanisms underlying intercellular infection with Frankia bacteria, we set up an efficient genetic transformation protocol for D. trinervis based on Agrobacterium rhizogenes. We showed that composite plants with transgenic roots expressing green fluorescent protein can be specifically and efficiently nodulated by Frankia strain BCU110501. Nitrogen fixation rates and feedback inhibition of nodule formation by nitrogen were similar in control and composite plants. In order to challenge the transformation system, the MtEnod11 promoter, a gene from Medicago truncatula widely used as a marker for early infection-related symbiotic events in model legumes, was introduced in D. trinervis. MtEnod11::GUS expression was related to infection zones in root cortex and in the parenchyma of the developing nodule. The ability to study intercellular infection with molecular tools opens new avenues for understanding the evolution of the infection process in nitrogen-fixing root nodule symbioses. 相似文献
86.
Schulte-Hostedde AI Kuula S Martin C Schank CC Lesbarrères D 《Journal of evolutionary biology》2011,24(5):1154-1159
Allometry of secondary sexual traits has been the subject of recent debate, and the generality of positive allometry and its association with sexual selection have been recently questioned. Whereas some studies suggest an almost universal positive allometry for traits under sexual selection and isometry or a negative allometry for traits not under such pressure, other studies argue that this pattern results from the study of exaggerated (ornamental) traits. To answer the call for an examination of the allometry of less-exaggerated sexually selected traits, we have examined morphological data from 14 sexually dimorphic traits and six monomorphic traits from three anuran species. Although we found evidence of positive allometry in male secondary sexual traits of several species and populations, not all nonsexual traits were isometric or exhibited negative allometry. Furthermore, our results indicate that larger traits in the populations that we studied were not associated with greater allometric slopes. Therefore, our study is in line with the contention suggesting no specific kind of allometric pattern for sexual and nonsexual characters, and we can only advocate for further investigation of trait allometry and sexual selection to understand the complexity underlying the evolution of allometry in sexual traits. 相似文献
87.
Cortes-Bratti X Bassères E Herrera-Rodriguez F Botero-Kleiven S Coppotelli G Andersen JB Masucci MG Holmgren A Chaves-Olarte E Frisan T Avila-Cariño J 《PloS one》2011,6(2):e16960
Background
Thioredoxin 80 (Trx80) is an 80 amino acid natural cleavage product of Trx, produced primarily by monocytes. Trx80 induces differentiation of human monocytes into a novel cell type, named Trx80-activated-monocytes (TAMs).Principal Findings
In this investigation we present evidence for a role of TAMs in the control of intracellular bacterial infections. As model pathogens we have chosen Listeria monocytogenes and Brucella abortus which replicate in the cytosol and the endoplasmic reticulum respectively. Our data indicate that TAMs efficiently inhibit intracellular growth of both L. monocytogenes and B. abortus. Further analysis shows that Trx80 activation prevents the escape of GFP-tagged L. monocytogenes into the cytosol, and induces accumulation of the bacteria within the lysosomes. Inhibition of the lysosomal activity by chloroquine treatment resulted in higher replication of bacteria in TAMs compared to that observed in control cells 24 h post-infection, indicating that TAMs kill bacteria by preventing their escape from the endosomal compartments, which progress into a highly degradative phagolysosome.Significance
Our results show that Trx80 potentiates the bactericidal activities of professional phagocytes, and contributes to the first line of defense against intracellular bacteria. 相似文献88.
Nidhi?P.?ChananaEmail author Vibha?Dhawan Sant?S.?Bhojwani 《Plant Cell, Tissue and Organ Culture》2005,83(2):169-177
Androgenesis is a phenomenon in which microspores are made to bypass the sexual pathway and follow the sporophytic mode of development to generate new plants without the intervention of fertilization under specialized in vitro conditions. Microspore culture provides an ideal system, with a large, relatively uniform population of haploid cells, for use in mutant selection, genetic transformation and in studies on the molecular mechanism of induction of androgenesis and embryogenesis. This paper involves a study on establishing a reproducible and efficient protocol for microspore embryogenesis in various varieties of Brassica juncea. The genotype had a pronounced effect on androgenic response in microspore cultures. The cultivar Rajat exhibited the most response, producing around 3500 embryos/100 buds. The microspores of B. juncea cv. PR-45 from ed plants maintained at a day/night temperature of 10 °C/5 °C form embryos with suspensors with varied morphology. The microspore embryos germinated to produce plants with frequencies. These plants exhibited 52% survival and 74% fertility. 相似文献
89.
A method for microperfusion of isolated segments of the midgut epithelium of Drosophila larvae has been developed to characterize cellular transport pathways and membrane transporters. Stereological ultrastructural
morphometry shows that this epithelium has unusually long tight junctions, with little or no lateral intercellular volume
normally found in most epithelia. Amplification of the apical and basal aspects of the cells, by ≈ 17-fold and ≈ 7-fold, respectively,
predicts an almost exclusively transcellular transport system for solutes. This correlates with the high lumen-negative transepithelial
potential (Vt) of 38 to 45 mV and high resistance (Rt) of 800 to 1400 Ω • cm2 measured by terminated cable analysis, in contrast to other microperfused epithelia like the renal proximal tubule. Several
blockers (amiloride 10−4 M, ouabain 10−4 M, bumetanide 10−4 M), K+-free solutions, or organic solutes such as D-glucose 10 mM or DL-alanine 0.5 mM failed to affect Vt or Rt. Bafilomycin-A1 (3 to 5 μM) decreased Vt by ≈ 40% and short-circuit current (Isc) by ≈ 50%, and decreased intracellular pH when applied from the basal side only, consistent with an inhibition of an electrogenic
V-H+-ATPase located in the basal membrane. Gradients of H+ were detected by pH microelectrodes close to the basal aspect of the cells or within the basal extracellular labyrinth. The
apical membrane is more conductive than the basal membrane, facilitating secretion of base (presumably HCO3−), driven by the basal V-H+-ATPase. 相似文献
90.
Urmil?J.?Mehta N.?Sahasrabudhe Sulekha?HazraEmail author 《In vitro cellular & developmental biology. Plant》2005,41(3):240-243
Summary Germination of tamarind seeds in medium containing thidiazuron (TDZ) resulted in induction of nodular protrusions in and around
the cotyledonary node meristem. The structures developed radially in well-defined circles and subsequently spread towards
the cotyledonary bridge and also in the proximal part of the hypocotyl. The structures developed into shoots on transfer to
medium devoid of growth regulators. Histological studies revealed that the protrusions initiated from the nodal meristem and
extended to the non-meristematic region between the two meristems and also in the proximal part of the hypocotyl in seedlings
germinated in 9.08 μM TDZ. Newly formed cell layers and less-differentiated meristematic protrusions were also seen. With the increase in the distance
from the meristem, the buds were less differentiated; in the proximal part of the hypocotyl only the multiple layers of meristematic
cells were noted. With extension of the period of incubation, the TDZ-induced meristematic activity extended laterally in
circles towards the neighboring region. The radial spread of the meristematic activity from the center of the nodal meristem
was also evident at 18.16 μM TDZ. From the pattern of the morphogenic development and the histological studies it may be hypothesized that in tamarind,
TDZ influences the existing meristems specifically. Subsequently de novo organogenesis is triggered in the neighboring cells. 相似文献