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161.
The uptake of 2-deoxy-d-glucose (2-DG) into human glioma cells (138 MG) was related to cell growth. The uptake of 2-DG was high at confluency but low in both rapidly growing sparse cultures and in growth-inhibited dense cultures. Lineweaver-Burke plots of uptake at different cell densities showed changes in Vmax; Km, however, remained constant. Dibutyryl cyclic-AMP (db-cAMP) doubled the uptake of 2-DG into rapidly growing sparse cultures but lacked effect at higher cell density. Independent of their density, cells treated with db-cAMP attained the characteristic morphology of differentiated glial cells. 相似文献
162.
Francisco J. Silva Luisa M. Botella Jan-Erik Edström 《Journal of molecular evolution》1990,31(3):221-227
Summary An interspecies comparison was made between the 3 ends of Balbiani ring genes fromChironomus. The comparison was focused on the BR2.2 gene, and a part at the 3 end fromChironomus pallidivittatus (which included also a segment of the gene core) was cloned. Its sequence, and other previously published BR sequences from this species and fromChironomus tentans were used in the analysis.The 3 parts of these repetitive genes can be divided into a region belonging to the core of the genes followed by a terminal region. In the core region the repeats (each of which consists of a constant part and a subrepeated part) are highly similar and the constant parts show little interspecies differentiation. Furthermore, the two parts of the repeats are units in an evolutionary and probably also functional sense.The terminal region contains modified constant units, usually isolated betwen acidic so-called cys regions, the whole arrangement lying upstream of an intron toward a 3-terminal exon. Most of the modified constant units are mosaics in rates of evolution with stable outer quarters bordering to equally stable cys regions and a central half with a very high rate of evolution. One of the terminal units, present only in the BR2.2 gene and second from the end, differs distinctly not only from corresponding core units but also from other terminal units in the three normally active BR genes. It lies upstream of all cys regions and is evolutionarily conserved over most of its length.Furthermore, two-dimensional protein structure prediction does not exclude an endoproteolytic cleavage site in this unit. Such a site appears unlikely in other terminal or core regions. This is of interest in view of evidence for intracellular cleavage of the BR2.2 terminal region with liberation of a part containing a DNA-binding domain (Botella et al. 1988).All in all the fine anatomy of evolutionary changes at the BR gene termini shows interesting correlations with postulated functional relations and may have predictive value in the further functional analysis of this part of the gene. 相似文献
163.
Tandemly repeated 340 bp sequences, TA repeats, are present in seven of the eight pairs of chromosome ends in Chironomus pallidivittatus, being absent from the telocentric left end of chromosome four. We have previously shown that the family of TA repeats consists of four main subfamilies. One subfamily is composed of a master unit and the other three contain derived units, each of which has a small region where the master sequence is highly mutated. Here we find that there are considerable variations in numbers of TA repeats between animals and for the same telomere in different animals. We also show that the seven telomere pairs containing TA repeats differ with regard to the content of derived subfamilies. The master unit is probably present in all seven pairs. Two of the derived units are exclusively present in two telomere pairs. The third derived unit shows a more irregular distribution. Some of the telomeres have highly variable contents of such units among animals. Subfamilies thus have different behaviour as reflected in their stable and variable patterns of distribution between individual telomeres.W. Hennig 相似文献
164.
Electron probe X-ray microanalysis of human muscle biopsies 总被引:5,自引:0,他引:5
Romuald Wroblewski Godfried M. Roomans Eva Jansson Lars Edström 《Histochemistry and cell biology》1978,55(4):281-292
Summary The elemental composition of human muscle fibres have been determined by electron probe microanalysis. In order to distinguish between different types of fibres, two approaches were used. In one approach individual fibres were isolated, portions of them used for a typing by histochemical methods and the main part used for X-ray microanalysis. In the other approach the muscle biopsy was serial-sectioned, some sections used for a histochemical typing and the others (16 m thick cryosections) used for X-ray microanalysis in the electron microscope.The comparison of the ratios between P, S and K in Study No. 1 and 2 indicates different concentrations of sulphur in the subsarcolemmal zone and in the interior of the fibre. Both routes give information on all elements (except the ten lightest ones) contained in the fibres or in sections of them, provided the concentration is high enough. In order to obtain quantitative data, expressed as mmol/kgdw, the spectra of the specimens were compared to those of standards of known composition and the data subjected to a so called ZAF-correction (corrections for the atomic number effect, absorption of X-rays in the specimen and secondary fluorescence). Quantitative data concerning phosphorus, sulphur, chlorine and potassium were obtained in Study No. 2. A significantly higher sulphur concentration was found in type IIA muscle fibres as compared to those of type I. 相似文献
165.
166.
Binding of complement subcomponent C1q to Streptococcus pyogenes: evidence for interactions with the M5 and FcRA76 proteins 总被引:1,自引:0,他引:1
Irina V Koroleva ers G Sjöholm Claes Schalén 《FEMS immunology and medical microbiology》1998,20(1):11-20
Binding of C1q, the first component of the complement system, to some human pathogens has been earlier reported. In the present study, direct binding of C1q to group A streptococci (GAS) of various serotypes as well as some other Gram-positive and Gram-negative species was demonstrated. The interaction between C1q and GAS was investigated more in detail. In hot neutral extracts of a number of GAS strains two components of 64 and 52 kDa, respectively, bound C1q; alkaline and SDS extracts yielded the 52 kDa component as the main C1q-binding substance. Trypsin treatment of the SDS extracts of two GAS strains suggested the C1q-binding component(s) to be of protein nature. C1q-binding material purified from the SDS extract of an avirulent strain, type T27, was separated in 12% SDS-PAGE and probed in Western blot with human C1q and fibrinogen, conjugated to horse radish peroxidase (HRP) as well as rabbit IgG antibodies complexed to HRP (PAP system). The 52 kDa component was non-reactive with fibrinogen or rabbit IgG. However, C1q-binding components purified from the alkaline extracts of two M-positive strains revealed strong binding of either fibrinogen (type M5) or both fibrinogen and rabbit IgG (type M76); the molecular mass of these components, 55 kDa and 43–40 kDa, respectively, was in agreement with the reported molecular mass of the M5 and FcRA76 proteins. Our findings suggest that C1q may interact with GAS through certain M-family proteins as well as by a so far unidentified surface factor of protein nature occurring in most GAS strains. The involvement of M-family proteins, regarded as virulence factors of these organisms, may suggest the interaction of GAS with C1q as biologically important. 相似文献
167.
Igor Golovliov Kerstin Kuoppa ers Sjöstedt Arne Tärnvik Gunnar Sandström 《FEMS immunology and medical microbiology》1996,13(3):239-244
Abstract Cytokine mRNA expression was determined in the liver of mice subcutaneously inoculated with a lethal dose of the highly virulent strain FSC 041 of Francisella tularensis subvar. tularensis or a sublethal dose of the live vaccine strain of F. tularensis subvar. palaearctica . Expression of mRNA for TNF-α, IL-12, IFN-γ, and IL-10 was demonstrated within 48 h of inoculation, the kinetics being similar irrespective of bacterial strain used. Thus, the expression of a cytokine response believed to be important in the early host defence against live vaccine strain seemed insufficient to prevent the lethality of a more virulent strain. 相似文献
168.
Physiological overlap between osmotolerance and thermotolerance in Saccharomyces cerevisiae 总被引:3,自引:0,他引:3
Christina Trollmo Lars André ers Blomberg Lennart Adler 《FEMS microbiology letters》1988,56(3):321-325
Abstract A convenient enzyme-linked immunoabsorbent assay (ELISA) to titrate serum immunoglobin G to Campylobacter pylori (Cp) is described. It was found that 80% of the serum samples obtained from patients with Cp-associated chronic gastritis ( N = 476) displayed statistically raised IgG anti-Cp, as compared to healthy volunteer controls ( N = 24). Titers in patient sera were statistically higher than the average control value plus twice the standard deviation, thus supporting Cp involvement in these gastric diseases. Also, ELISA inhibition experiments suggested that antigenic variation among various Cp isolates is likely, thus indicating the need for subgrouping of Cp. We note the usefulness of the above IgG anti-Cp ELISA for sero-epidemiological studies of Cp associated chronic gastritis, especially those tracing the source of Cp infections and those determining potentially disease related Cp subgroups. 相似文献
169.
Erica Brostedt ers Lindblad Janet Jansson Stefan Nordlund 《FEMS microbiology letters》1997,150(2):263-267
The role of the reactions of the TCA cycle in the generation of reductant for nitrogenase in Rhodospirillum rubrum has been investigated. Addition of fluoroacetate inhibited nitrogenase activity almost completely when pyruvate or endogenous sources were used as electron donors, whereas the inhibition was incomplete when malate, succinate or fumarate were used. Addition of NAD(P)H to cells supported nitrogenase activity, both with and without prior addition of fluoroacetate. We suggest that the role of the TCA cycle in nitrogen fixation in R. rubrum is to generate reduced pyridine nucleotides which are oxidized by the components of the electron transport pathway to nitrogenase. 相似文献
170.
Johannes Salamon Tatjana Hoffmann Eva Elies Kersten Peldschus Julia S. Johansen Georg Lüers Udo Schumacher Daniel Wicklein 《PloS one》2014,9(4)
Induced overexpression of the secretory protein YKL-40 promotes tumor growth in xenograft experiments. We investigated if targeting YKL-40 with a monoclonal antibody could inhibit tumor growth. YKL-40 expressing human melanoma cells (LOX) were injected subcutenously in Balb/c scid mice. Animals were treated with intraperitoneal injections of anti-YKL-40, isoptype control or PBS. Non-YKL-40 expressing human pancreatic carcinoma cell line PaCa 5061 served as additional control. MR imaging was used for evaluation of tumor growth. Two days after the first injections of anti-YKL-40, tumor volume had increased significantly compared with controls, whereas no effects were observed for control tumors from PaCa 5061 cells lacking YKL-40 expression. After 18 days, mean tumor size of the mice receiving repeated anti-YKL-40 injections was 1.82 g, >4 times higher than mean tumor size of the controls (0.42 g). The effect of anti-YKL-40 on the increase of tumor volume started within hours after injection and was dose dependent. Intratumoral hemorrhage was observed in the treated animals. The strong effect on tumor size indicates important roles for YKL-40 in melanoma growth and argues for a careful evaluation of antibody therapy directed against YKL-40. 相似文献