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991.
J C van der Meulen 《Plastic and reconstructive surgery》1992,89(6):1060-1067
Flattening of the nasal tip and shortness of the columella are two of the deformities that remain following successful repair of a bilateral cleft of the lip. Until now, correction has not been possible without producing undesirable scars on the surface of the nose or lip. A three-dimensional Z-plasty on the alar rim achieves columellar lengthening and forward projection of the tip, but it does not have these disadvantages. 相似文献
992.
Walter W. Ferguson 《Primates; journal of primatology》1992,33(2):273-279
In response to a critique byFerguson (1989),Leonard (1991) reiterates most of his original arguments for supporting “Australopithecus afarensis”Johanson, White, andCoppens, 1978 as a single species. He disregards the principle of morphological equivalence by comparing the dental metrics and morphology
of a hominid with those of species of the Pongidae, which do not correspond with the degree of variation in hominids, instead
of with those of species of the Hominidae. He fails to refute clear evidence that the range of variation of dental metrics
and morphology in “A. afarensis” exceeds that seen in species of the Hominidae. On the basis of extreme variation, “A. afarensis” is, therefore, interpreted as representing a composite species. 相似文献
993.
M J van der Werf W J van den Tweel S Hartmans 《Applied and environmental microbiology》1992,58(9):2854-2860
More than 300 microorganisms were screened for their ability to convert maleate into D-malate as a result of the action of maleate hydratase. Accumulation of fumarate during incubation of permeabilized cells with maleate was shown to be indicative of one of the two enzymes known to transform maleate. The ratio in which fumarate and malate accumulated could be used to estimate the enantiomeric composition of the malate formed. Many strains (n = 128) were found to be capable of converting maleate to D-malate with an enantiomeric purity of more than 97%. Pseudomonas pseudoalcaligenes NCIMB 9867 was selected for more detailed studies. Although this strain was not able to grow on maleate, permeabilized cells were able to degrade maleate to undetectable levels, with a concomitant formation of D-malate. The D-malate was formed with an enantiomeric purity of more than 99.97%. 相似文献
994.
Walter Borzani Haroldo Hiss Teresinha W. de Santos Marina L. R. Vairo 《Biotechnology letters》1992,14(10):981-984
Summary A mathematical model is proposed to explain the influence of the volume fraction of inoculum on the fermentation time and ethanol productivity in semicontinuous ethanol fermentation of sugar cane blackstrap molasses by pressed yeast.Nomenclature a, b, c, d
constants, see equation (5)
- Eo
initial ethanol concentration
- Ef
final ethanol concentration
- K1, K2, K3
constants, see equation (1)
- P
ethanol productivity
- Pc
calculated values of P
- Pe
experimental values of P
- r
correlation coefficient
- So
initial TRS concentration
- Sm
TRS concentration of the feeding mash
- T
fermentation time (average of the experimental values)
- Tc
calculated value of T
- Te
experimental value of T
- TRS
total reducing sugars calculated as glucose
- Uo
initial urea concentration
- Um
urea concentration of the feeding mash
- V
reactor working volume
- Vi
volume of the inoculum
-
volume fraction of inoculum=Vi/V 相似文献
995.
Walter A. Blank Kenneth J. Elder Wendy P. Gati Alan R. P. Paterson Michael A. Pickard John S. Wilson 《Biotechnology letters》1992,14(8):669-672
Summary Washed cells ofE. coli ATCC 5275, a thymine auxotroph, catalysed formation of 2-chloro-2-deoxyadenosine when incubated with 2-chloroadenosine and a variety of deoxynucleosides. This transdeoxyribosylation reaction was complete after 4 h of shaking at 37°C. The equilibrium reaction mixture favoured product formation when purine rather than pyrimidine deoxyribonucleosides were used as cosubstrates, and when the ratio of deoxysugar donor to 2-chloroadenosine was high. Using deoxyadenosine as cosubstrate, chlorodeoxyadenosine was purified from larger scale reaction mixtures by treatment with Dowex-1 (OH-form) or by high performance liquid chromatography. 相似文献
996.
997.
In this paper, it is suggested that specificity and non-specificity in (oral) microbial adhesion are different expressions for the same phenomena. It is argued that the same basic, physicochemical forces are responsible for so-called 'non-specific' and 'specific' binding and that from a physico-chemical point of view the distinction between the two is an artificial one. Non-specific interactions arise from Van der Waals and electrostatic forces and hydrogen bonding, and originate from the entire cell. A specific bond consists of a combination of the same type of Van der Waals and electrostatic forces and hydrogen bonding, now originating from highly localized chemical groups, which together form a stereochemical combination. The absence or presence of specific receptor sites on microbial cell surfaces must therefore be reflected in the overall, non-specific surface properties of cells as well. This point is illustrated by showing that glucan-binding lectins on mutans streptococcal strains may determine the pH dependence of the zeta potentials of these cells. When studying microbial adhesion, a non-specific approach may be better suited to explain adhesion to inert substrata, whereas a specific approach may be preferred in case of adhesion to adsorbed protein films. Adhesion is, however, not as important in plaque formation in the human oral cavity as is retention, because low shear force periods, during which adhesion presumably occurs, are followed by high shear force periods, during which adhering cells must withstand these detachment forces. Evidence is provided that such detachment will be through cohesive failure in the pellicle mass, the properties of which are conditioned by the overall, non-specific substratum properties. Therefore, in vivo plaque formation may be more readily explained by a non-specific approach. 相似文献
998.
Summary Thirty-four wines that showed problems during malolactic fermentation were obtained from five different German wineries and were examined for the presence of phages using electron microscopy and the agar spot-test. Phages were discovered in 11 of the wines and host strains were found for ten of these phages. The phages were characterized on the basis of their morphology, host range and protein profiles. Furthermore the ten phages could be divided into four groups by restriction enzyme analysis of the phage DNA. This grouping was consistent with results based on morphology, protein composition and host range analysis.
Correspondence to: E. K. Arendt 相似文献
999.
Lysozyme expression in Lactococcus lactis 总被引:1,自引:0,他引:1
Maarten van de Guchte Fimme Jan van der Wal Jan Kok Gerard Venema 《Applied microbiology and biotechnology》1992,37(2):216-224
Summary Three lysozyme-encoding genes, one of eukaryotic and two of prokaryotic origin, were expressed in Lactococcus lactis subsp. lactis. Hen egg white lysozyme (HEL) could be detected in L. lactis lysates by Western blotting. No lysozyme activity was observed, however, presumably because of the absence of correctly formed disulphide bonds in the L. lactis product. The functionally related lysozymes of the E. coli bacteriophages T4 and were produced as biologically active proteins in L. lactis. In both cases, the highest expression levels were obtained using configurations in which the bacteriophage lysozyme genes had been translationally coupled to a short open reading frame of lactococcal origin. Both enzymes, like HEL, may prevent the growth of food-spoilage bacteria. 相似文献
1000.
Summary Synthetic oligonucleotides encoding the 5-non-translated (leader) sequence of the coat protein mRNA of alfalfa mosaic virus RNA 4 or the leader sequence of tobacco mosaic virus RNA were used to replace the natural leader region of the yeast phosphoglycerate kinase (PGK1) mRNAs and the translational efficiency of the chimeric mRNA was determined in yeast cells. In neither case did we observed a significant increase compared to the translational efficiency shown by the wild-type PGK mRNA, in contrast to the known stimulatory effect of these leader sequences on translation in mammalian, plant and bacterial in-vivo and/or in-vitro systems. The same result was obtained when the translational efficiencies in yeast cells of Escherichia coli -galactosidase mRNAs carrying the PGK or either of the two viral leader sequences were compared.
Offprint requests to: H. A. Raué 相似文献