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981.
Maria Carmen del Ruiz Ruiz Christian Stanetty Laszlo Czollner Ulrich Jordis Dirk Claßen-Houben Paul Kosma 《Carbohydrate research》2009,344(9):1063-1071
3-O-Glycopyranosides of glycyrrhetinic acid have been synthesized in good to high yields and excellent stereoselectivity using glycosyl bromide donors and silver zeolite as promoter. In addition to the preparation of glycosides containing β-linked glucosyl, 2-deoxy-2-trichloroacetamido-glucosyl, galactosyl, cellobiosyl and lactosyl residues, also the deactivated acetylated methyl glucopyranosyluronate bromide donor could be coupled to triterpene aglycon ester derivatives in good yields. The ester protecting group located at C-30 of the oleanolic acid scaffold exerted an influence on the overall yield, with the methylester-protected glycosyl acceptor giving better yields compared to the allyl, benzyl as well as diphenylmethyl ester aglycon. The acetyl-protected glucuronides were differently deblocked in high yields via Zemplén deacetylation or via hydrogenolysis followed by Zemplén deacetylation, and alkaline hydrolysis, respectively, to allow for a selective liberation of the ester groups from either the glucuronide or the glycyrrhetinic acid unit, respectively. The target glycosides/glucuronides serve as probes for pharmaceutical studies aimed at defining structure-activity relationships of glycoside/glucuronide triterpenes. 相似文献
982.
In microvillar photoreceptors, light stimulates the phospholipase C cascade and triggers an elevation of cytosolic Ca2+ that is essential for the regulation of both visual excitation and sensory adaptation. In some organisms, influx through light-activated ion channels contributes to the Ca2+ increase. In contrast, in other species, such as Lima, Ca2+ is initially only released from an intracellular pool, as the light-sensitive conductance is negligibly permeable to calcium ions. As a consequence, coping with sustained stimulation poses a challenge, requiring an alternative pathway for further calcium mobilization. We observed that after bright or prolonged illumination, the receptor potential of Lima photoreceptors is followed by the gradual development of an after-depolarization that decays in 1–4 minutes. Under voltage clamp, a graded, slow inward current (Islow) can be reproducibly elicited by flashes that saturate the photocurrent, and can reach a peak amplitude in excess of 200 pA. Islow obtains after replacing extracellular Na+ with Li+, guanidinium, or N-methyl-d-glucamine, indicating that it does not reflect the activation of an electrogenic Na/Ca exchange mechanism. An increase in membrane conductance accompanies the slow current. Islow is impervious to anion replacements and can be measured with extracellular Ca2+ as the sole permeant species; Ba can substitute for Ca2+ but Mg2+ cannot. A persistent Ca2+ elevation parallels Islow, when no further internal release takes place. Thus, this slow current could contribute to sustained Ca2+ mobilization and the concomitant regulation of the phototransduction machinery. Although reminiscent of the classical store depletion–operated calcium influx described in other cells, Islow appears to diverge in some significant aspects, such as its large size and insensitivity to and lanthanum; therefore, it may reflect an alternative mechanism for prolonged increase of cytosolic calcium in photoreceptors. SKF96365相似文献
983.
Andre-i Sarabia-Sainz Gabriela Ramos-Clamont Ma. María del Carmen Candia-Plata Luz Vázquez-Moreno 《International journal of biological macromolecules》2009,44(2):175-181
Escherichia coli (E. coli) that expresses galactose-reactive lectins, like K88 adhesin, causes high mortality among piglets. Carbohydrates that compete for adhesion could serve as an alternative for disease prevention. Porcine serum albumin (PSA) was modified by non-enzymatic glycation with lactose to produce PSA-Lac or PSA-Glc β (1–4) Gal, as confirmed by reduction of available free amino groups, increased molecular mass and by Ricinus communis lectin recognition. E. coli K88 binds to PSA-Lac treatments containing three and four lactoses, respectively. In addition, PSA-Lac partially inhibited K88 strain adherence to mucins. These results suggest that neoglycoconjugates obtained by non-enzymatic glycation of proteins may serve in the prophylaxis of piglets’ diarrhea. 相似文献
984.
In the present study we intended to determine how BAT (brown adipose tissue) maintained thermogenesis under treatment with OE (oleoyl-oestrone), a powerful slimming hormone that sheds off body lipid but maintains the metabolic rate. Overweight male rats were subjected to daily gavages of 10 nmol/g of OE or vehicle (control) for 10 days. A PF (pair-fed) vehicle-receiving group was used to discount the effects attributable to energy availability limitation. Interscapular BAT mass, lipid, DNA, mRNA and the RT-PCR (real-time PCR) expression of lipid and energy metabolism genes for enzymes and regulatory proteins were measured. BAT mass and lipid were decreased in OE and PF, with the latter showing a marked reduction in tissue mRNA. Maintenance of perilipin gene expression in PF and OE rats despite the loss of lipid suggests the preservation of the vacuolar interactive surface, a critical factor for thermogenic responsiveness. OE and, to a lesser extent, PF maintained the expression of genes controlling lipolysis and fatty acid oxidation, but markedly decreased the expression of those genes involved in lipogenic and acyl-glycerol synthesis. OE did not affect UCP1 (uncoupling protein 1) (decreased in PF), beta(3) adrenergic receptors or hormone-sensitive lipase gene mRNAs, which may translate in maintaining a full thermogenic system potential. OE rats were able to maintain a less energetically stressed BAT (probably through glucose utilization) than PF rats. These changes were not paralleled in PF rats, in which lower thermogenesis and glucose preservation resulted in a heavier toll on internal fat stores. Thus the mechanism of action of OE is more complex and tissue-specific than previously assumed. 相似文献
985.
Juan Manuel Ruiz-Lozano Maria del Mar Alguacil Gloria Bárzana Paolo Vernieri Ricardo Aroca 《Plant molecular biology》2009,70(5):565-579
The arbuscular mycorrhizal (AM) symbiosis has been shown to modulate the same physiological processes as the phytohormone
abscisic acid (ABA) and to improve plant tolerance to water deficit. The aim of the present research was to evaluate the combined
influence of AM symbiosis and exogenous ABA application on plant root hydraulic properties and on plasma-membrane intrinsic
proteins (PIP) aquaporin gene expression and protein accumulation after both a drought and a recovery period. Results obtained
showed that the application of exogenous ABA enhanced osmotic root hydraulic conductivity (L) in all plants, regardless of water conditions, and that AM plants showed lower L values than nonAM plants, a difference that was especially accentuated when plants were supplied with exogenous ABA. This
effect was clearly correlated with the accumulation pattern of the different PIPs analyzed, since most showed reduced expression
and protein levels in AM plants fed with ABA as compared to their nonAM counterparts. The possible involvement of plant PIP
aquaporins in the differential regulation of L by ABA in AM and nonAM plants is further discussed. 相似文献
986.
Francisco O'Valle Raimundo G. M. Del Moral María del Carmén Benítez David Martín-Oliva Mercedes Gómez-Morales David Aguilar José Aneiros-Fernández Pedro Hernández-Cortés Antonio Osuna Francesc Moreso Daniel Serón Francisco J. Oliver Raimundo G. Del Moral 《PloS one》2009,4(9)
Cold ischemia time especially impacts on outcomes of expanded-criteria donor (ECD) transplantation. Ischemia-reperfusion (IR) injury produces excessive poly[ADP-Ribose] Polymerase-1 (PARP-1) activation. The present study explored the hypothesis that increased tubular expression of PARP-1 contributes to delayed renal function in suboptimal ECD kidney allografts and in non-ECD allografts that develop posttransplant acute tubular necrosis (ATN).
Materials and Methods
Nuclear PARP-1 immunohistochemical expression was studied in 326 paraffin-embedded renal allograft biopsies (193 with different degrees of ATN and 133 controls) and in murine Parp-1 knockout model of IR injury.Results
PARP-1 expression showed a significant relationship with cold ischemia time (r coefficient = 0.603), time to effective diuresis (r = 0.770), serum creatinine levels at biopsy (r = 0.649), and degree of ATN (r = 0.810) (p = 0.001, Pearson test). In the murine IR model, western blot showed an increase in PARP-1 that was blocked by Parp-1 inhibitor. Immunohistochemical study of PARP-1 in kidney allograft biopsies would allow early detection of possible delayed renal function, and the administration of PARP-1 inhibitors may offer a therapeutic option to reduce damage from IR in donor kidneys by preventing or minimizing ATN. In summary, these results suggest a pivotal role for PARP-1 in the ATN of renal transplantation. We propose the immunohistochemical assessment of PARP-1 in kidney allograft biopsies for early detection of a possible delayed renal function. 相似文献987.
Sandro Bogdanović Salvatore Brullo Gianpietro Giusso del Galdo Cristina Salmeri 《Folia Geobotanica》2009,44(1):83-93
Here we describe Allium telmatum Bogdanovi?, Brullo, Giusso & Salmeri, a new species of Allium sect. Codonoprasum from North Dalmatia (Croatia). Its chromosome number (2n?=?32), karyotype, leaf anatomy, ecology and taxonomical relationships are examined. Several features, such as phenology (flowering in autumn), occurrence in coastal salt marshes, tetraploid chromosome number, and morphology, indicate that it is most closely related to the Tyrrhenian species Allium savii. On the basis of our herbarium survey, we present here a distribution map of the autumn-flowering species of Allium sect. Codonoprasum in the Mediterranean area. 相似文献
988.
Hugo Palafox Julio H. Córdova-Murueta María A. Navarrete del Toro Fernando L. García-Carreño 《Process Biochemistry》2009,44(5):584-587
There is a growing interest in adding value to the jumbo squid Dosidicus gigas fishery. This work describes two extraction procedures for processing muscle to obtain protein isolates with suitable functional properties. The effect on muscle protein solubility and protein recovery of combining freezing and grinding raw materials during storage was evaluated. Processes are based on extraction of protein at acid or alkaline pH and subsequent iso-electric precipitation. About 85% of the initial muscle protein was solubilized at pH 3 and 11. Regardless of the pH used for extraction, about 90% of the protein was obtained after precipitation at pH 5.5. The total yield from both procedures was 75%. Treatments during storage did not significantly affect solubility and yield of protein. Wastewater contained negligible amounts of protein and may be reused. Processing by acid and alkaline extraction are feasible alternatives for obtaining protein isolates either from fresh or frozen squid muscle, which is an important consideration when choosing the most appropriate and inexpensive method to scale up this technology. 相似文献
989.
Cristina Valls Setefilla Molina Teresa Vidal Jos C. del Río Josep F. Colom ngel T. Martínez Ana Gutirrez M. Blanca Roncero 《Process Biochemistry》2009,44(9):1032-1038
The way how sterols, the main lipophilic compounds present in eucalypt kraft pulp, are eliminated by an enzymatic stage using the laccase-mediator system was evaluated. With this purpose laccase-mediator stage (L) was applied on an Eucalyptus globulus pulp under different operation conditions following a three-variable (laccase dose, mediator dose and reaction time) sequential statistical plan, to optimise the removal of sterols. The decrease in pulp sterol content during the enzymatic treatment was related to the decrease in kappa number and to brightness increase, as well as with the increase in some oxidation products of sitosterol (namely 7-oxositosterol and stigmasta-3,5-dien-7-one). The increase in reaction time from 1 to 5 h strongly reduced the sterol content, while no more sterols were eliminated during the 5–7 h period. Increasing the laccase dose from 1 to 20 U g−1 of pulp produced a high reduction in pulp sterols, whereas the increase in mediator (1-hydroxybenzotriazole) dose (from 0.5 to 2.5% of pulp weight) had only a slight influence in removing sterols. Therefore, at 16 U g−1 laccase dose, 0.5% mediator dose, 4 h of reaction, practically all the sterols were removed. Finally, it was demonstrated that sterols were more sensitive to a L stage (practically 100% of sterols were eliminated) than to a chlorine dioxide stage (54% of sterols eliminated). 相似文献
990.
María del Mar Muñío Alfonso Robles Luis Esteban Pedro A. González Emilio Molina 《Process Biochemistry》2009,44(7):723-730
This paper studies the synthesis of structured triacylglycerols (STAGs), rich in polyunsaturated fatty acids (PUFAs) by a two-step enzymatic process: (i) alcoholysis of fish oils (cod liver and tuna oils) with ethanol to obtain 2-monoacylglycerols (2-MAGs), catalyzed by 1,3 specific lipases and (ii) esterification of these 2-MAGs with caprylic acid (CA, 8:0), also catalyzed by a 1,3 specific lipase, to produce STAGs of structure CA–PUFA–CA. As regards the alcoholysis reaction, three factors have been studied: the influence of the type of lipase used (lipase D from Rhizopus oryzae, immobilized on Accurel MP1000, and Novozym 435 from Candida antarctica), the operational mode of a stirred tank reactor (STR operating in discontinuous and continuous mode) and the intensity of treatment (IOT = lipase amount × reaction time/oil amount). Although higher 2-MAG yields were obtained with lipase D, Novozym 435 was selected due to its greater stability in the operational conditions. The highest 2-MAG yield (63%) was attained in the STR operating in discontinuous mode at an IOT of 1 g lipase × h g oil?1 (at higher IOT the 2-MAGs were degraded to glycerol). This system was scaled up to 100 times the initial volume, achieving a similar yield (65%) at the same IOT. The 2-MAGs in the final alcoholysis reaction mixture were separated from ethyl esters by solvent extraction using solvents of low toxicity (ethanol and hexane); the 2-MAG recovery yield was over 90% and the purity was approximately 87–90%. Regarding the esterification of the 2-MAGs, the following factors were studied: the influence of the lipase type used, the presence or absence of solvent (hexane) and the reaction time or intensity of treatment (IOT = lipase amount × reaction time/2-MAG amount). Of the five lipases tested, the highest STAG percentages (over 90%) were attained with lipases D and DF, immobilized on Accurel MP1000. These STAGs contain 64% CA, of which 98% is at positions 1 and 3. Position 2 contains 5% CA and 45% PUFAs, which means that all the PUFAs that were located at position 2 in the original oil remain in that position in the final STAGs. The lipase D immobilized on Accurel MP1000 is stable in the operational conditions used in the esterification reaction. Finally the purification of STAGs was carried out by neutralization of free fatty acids with hydroethanolic solution of KOH and extraction of STAGs with hexane. By this method purity was over 95% and separation yields were about 80%. 相似文献