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101.
On the active site of diamine oxidase: kinetic studies 总被引:1,自引:0,他引:1
102.
Abstract— Unilateral sympathetic decentralization of the superior cervical ganglion of rats was performed 3 days prior to the experiments. A two-compartment kinetic model was proposed to describe the effect of decentralization on (1) the uptake of a nonphysiological amino acid from plasma to the submaxillary gland and (2) the incorporation of a physiological amino acid from precursor pool into protein. The calculations based on the model showed that the fractional rate constant for efflux of the nonphysiological amino acid, α-[3-14 C] aminoisobutyric acid, was greater in the decentralized than in the normal gland. However, efflux rate was equal in the two glands because the extrapolated zero time value of the initial concentration was greater in the normal gland.
The labelled physiological amino acid, [14 C]leucine, was used in initial experiments to assess turnover rate of the gland proteins but it was rapidly metabolized to many other radioactive compounds. Therefore, arginine[14 C]guanido was employed-arginine being the only labelled amino acid found after injection. Since the steady state content of submaxillary gland proteins was not changed but the fractional rate constant of conversion of free arginine into protein (kp ) was greater in the decentralized gland (kp = 0-40 h_l ) than in the normal (kp = 0-27 h−1 ), we can conclude that decentralization increases protein turnover rate; thus, assuming that arginine[14 C]guanido is homogeneously distributed in the tissue pools of free arginine, the rate of protein turnover is greater in the sympathetically decentralized gland than in the normal. 相似文献
The labelled physiological amino acid, [
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Diamine oxidase inactivation by hydrogen peroxide 总被引:3,自引:0,他引:3
108.
Yellow water traps are often used for sampling populations of flying aphids. This note suggests that the size and probably the shape of traps should be standardised, because trapping efficiency (nos. caught/unit area), and the relative attractiveness of traps to different species, depends on trap size. Aphidologists using water traps to compare mixed populations of flying aphids, should therefore compare catches from different traps with caution.Traps of three sizes were made up from individual trays, each 29×21.5×5cm, and painted Hansa yellow inside; 1 tray alone, 4 arranged in a 2×2 rectangle, and 9 in a 3×3 rectangle, gave trapping surfaces of approx. 625, 2500 and 5700 cm2 respectively. The traps were half-filled with water plus a drop of detergent, placed on bare land and daily catches collected on 19 days in June and July 1966. 相似文献
109.
B Mondovì G Rotilio M T Costa A Finazzi-Agrò E Chiancone R E Hansen H Beinert 《The Journal of biological chemistry》1967,242(6):1160-1167
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