全文获取类型
收费全文 | 378156篇 |
免费 | 158723篇 |
国内免费 | 29753篇 |
专业分类
566632篇 |
出版年
2018年 | 5018篇 |
2016年 | 5895篇 |
2015年 | 7370篇 |
2014年 | 8349篇 |
2013年 | 10883篇 |
2012年 | 12152篇 |
2011年 | 12759篇 |
2010年 | 10962篇 |
2009年 | 15059篇 |
2008年 | 12200篇 |
2007年 | 12504篇 |
2006年 | 10854篇 |
2005年 | 10602篇 |
2004年 | 10651篇 |
2003年 | 9951篇 |
2002年 | 10583篇 |
2001年 | 18221篇 |
2000年 | 16093篇 |
1999年 | 17504篇 |
1998年 | 11972篇 |
1997年 | 11894篇 |
1996年 | 11104篇 |
1995年 | 11259篇 |
1994年 | 10724篇 |
1993年 | 10305篇 |
1992年 | 16350篇 |
1991年 | 15914篇 |
1990年 | 16551篇 |
1989年 | 15626篇 |
1988年 | 14411篇 |
1987年 | 13273篇 |
1986年 | 12130篇 |
1985年 | 11750篇 |
1984年 | 9460篇 |
1983年 | 8086篇 |
1982年 | 7238篇 |
1981年 | 6467篇 |
1980年 | 6231篇 |
1979年 | 8873篇 |
1978年 | 7118篇 |
1977年 | 6783篇 |
1976年 | 6320篇 |
1975年 | 6300篇 |
1974年 | 6894篇 |
1973年 | 6915篇 |
1972年 | 6816篇 |
1971年 | 6334篇 |
1970年 | 5513篇 |
1969年 | 5446篇 |
1968年 | 4699篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
101.
F. Kato T. Hino A. Nakaji M. Tanaka Y. Koyama 《Molecular & general genetics : MGG》1995,247(3):387-390
In many species of actinomycetes, carotenogenesis can be photoinduced. The capacity to respond to photoinduction is, however unstable and, in various strains of Streptomyces, is lost at a relatively high frequency. In Streptomyces setonii ISP5395, which normally produces no carotenoids, carotenoid-producing mutants can be obtained following protoplast regeneration. We report here the characterization of a gene, crtS, which was isolated from one such mutant and can confer on wild-type S. setonii ISP5395 cells the capacity to synthesize carotenoids. Sequence analysis of crtS reveals an open reading frame, which shows homology to genes that encode alternative sigma factors in Bacillus subtilis. We propose that crtS encodes a sigma factor which is necessary for the expression of a cryptic gene(s) for carotenoid biosynthesis in S. setonii ISP5395. 相似文献
102.
James B. Reid 《Animal behaviour》1982,30(4):1212-1216
A young captive rook, Corvus frugilegus, inserted a plug into a plug-hole in its aviary floor so that a pool of water formed, which was used by all four rooks in the aviary for drinking and bathing. The bird was selective about which, of six holes, it chose for insertion of the tool, choosing the appropriate one with respect to the water source. Days on which attempted or successful tool-use occurred were drier than other days, and days of successful tool-uses were also warmer. Availability of fresh water to the birds did not influence the occurrence of tool-use. 相似文献
103.
The insulin receptor substrates (IRSs)-1-4 play important roles in signal transduction emanating from the insulin and insulin-like growth factor (IGF)-I receptors. IRS-4 is the most recently characterized member, which has been found primarily in human cells and tissues. It interacts with SH2-containing proteins such as phosphatidylinositol 3'-kinase (PI3K), Grb2, Crk-II, and CrkL. In this study, we transfected IRS-4 in mouse NIH-3T3 cells that overexpress IGF-I receptors. Clones expressing IRS-4 showed enhanced cellular proliferation when cells were cultured in 1% fetal bovine serum without added IGF-I. Addition of IGF-I enhanced cellular proliferation in cells overexpressing the IGF-I receptor alone but had an even greater proliferative effect in cells overexpressing both the IGF-I receptors and IRS-4. When etoposide and methylmethane sulfonate (MMS), both DNA damaging agents, were added to the cells, they uniformly induced cell cycle arrest. Fluorescence-activated cell sorter analysis demonstrated that the arrest of the cell cycle occurred at the G(1) checkpoint, and furthermore no significant degree of apoptosis was demonstrated with the use of either agent. In cells, overexpressing IGF-I receptors alone, IGF-I addition enhanced cellular proliferation, even in the presence of etoposide and MMS. In cells overexpressing IGF-I receptors and IRS-4, the effect of IGF-I in overcoming the cell cycle arrest was even more pronounced. These results suggest that IRS-4 is implicated in the IGF-I receptor mitogenic signaling pathway. 相似文献
104.
105.
It has been proposed that amplification of genes for esterase that provide resistance to insecticides may originate from
transposition events. To test this hypothesis, we have constructed a minigene coding for a soluble acetylcholinesterase under
the control of a nontissue-specific promoter (hsp70). When introduced into Drosophila, the gene is expressed in all tissues and the extra acetylcholinesterase produced confers a low level of insecticide resistance
(twofold). The minigene was mobilized by crossing the initial transformant with a strain providing a source of P-element transposase.
After 34 generations of exposure to the organophosphate parathion, we obtained a strain with a higher resistance (fivefold).
This strain had only one extra Ace gene, which overexpressed acetylcholinesterase. Thus, following transposition, resistance resulted from the overexpression
of a single copy of the gene and not from gene amplification.
Received: 9 August 1996 / Accepted: 27 May 1997 相似文献
106.
Cysteine-proteinase activity was observed in homogenates of human-cadaver renal cortex. This activity co-purified with renin enzymic activity until separation by aminohexyl-Sepharose--pepstatin affinity chromatography. The cysteine proteinase was purified 1780-fold after the following successive chromatographic procedures: Sephadex G-75, DEAE-cellulose DE-52, and an organomercurial affinity resin. The proteinase activity was dependent upon activation by thiol-containing compounds such as dithiothreitol, as well as by EDTA, and was inhibited by the thiol-group-specific alkylating reagents iodoacetic acid and N-ethylmaleimide. DE-52 cellulose chromatography resolved the cysteine proteinase into two components. On the basis of molecular size (26 000 daltons), activity as a function of pH, stability as a function of pH, substrate specificity and thermal lability, the major component (95%) has been identified as cathepsin B. The DE-52 cellulose elution pattern of the minor component (5%) is suggestive of cathepsin H [Schwartz & Barrett (1980) Biochem. J. 191, 487-497] Enzymic activity was determined with synthetic substrates, in particular alpha-N-benzoyl-DL-arginine 2-naphthylamide (Bz-Arg-NNap), thus precluding the detection of cathepsin L [Kirschke, Langner, Wiederanders, Ansorge, Bohley & Broghammer (1976) Acta Biol. Med. Germ. 35, 285-299]. Inhibition by dimethyl sulphoxide was observed in the determination of Km = 7.0 +/- 0.4 mM for the substrate Bz-Arg-NNap, and care must therefore be taken in the preparation of substrate solutions. 相似文献
107.
108.
109.
Involvement of the galactosyl-1-phosphate transferase encoded by the Salmonella enterica rfbP gene in O-antigen subunit processing. 总被引:11,自引:5,他引:6 下载免费PDF全文
rfbT of Salmonella enterica LT2 was previously thought, together with rfaL, to be involved in the ligation of polymerized O antigen to core-lipid A, and three mutants were known. We report the mapping of the mutations to rfbP, the galactosyl-1-phosphate transferase gene, which is now shown to encode a bifunctional protein. The mutations which have the former rfbT phenotype are referred to as rfbP(T). We also show that rfbP(T) mutants are not blocked in the ligation step as previously believed but in an earlier step, possibly in flipping the O-antigen subunit on undecaprenyl pyrophosphate from the cytoplasmic to periplasmic face of the cytoplasmic membrane. 相似文献