全文获取类型
收费全文 | 74篇 |
免费 | 6篇 |
国内免费 | 2篇 |
专业分类
82篇 |
出版年
2022年 | 1篇 |
2020年 | 1篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 4篇 |
2013年 | 2篇 |
2012年 | 5篇 |
2011年 | 1篇 |
2010年 | 6篇 |
2009年 | 4篇 |
2008年 | 2篇 |
2007年 | 3篇 |
2006年 | 2篇 |
2005年 | 5篇 |
2004年 | 6篇 |
2002年 | 1篇 |
2001年 | 3篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 2篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1991年 | 3篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1981年 | 1篇 |
1977年 | 3篇 |
1970年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有82条查询结果,搜索用时 15 毫秒
51.
Replication-selective virotherapy for cancer: Biological principles, risk management and future directions. 总被引:22,自引:0,他引:22
In the search for novel cancer therapies that can be used in conjunction with existing treatments, one promising area of research is the use of viral vectors and whole viruses. This review describes the underlying biological principles and current status of the field, outlines approaches for improving clinical effectiveness and discusses the unique safety and regulatory issues surrounding viral therapies. 相似文献
52.
Inferences for a semiparametric model with panel data 总被引:1,自引:0,他引:1
53.
Olfaction is critical to the host preference selection behavior of many disease-transmitting insects, including the mosquito Anopheles gambiae sensu stricto (hereafter A. gambiae), one of the major vectors for human malaria. In order to more fully understand the molecular biology of olfaction in this insect, we have previously identified several members member of a family of candidate odorant receptor proteins from A. gambiae (AgORs). Here we report the cloning and characterization of an additional AgOR gene, denoted as AgOr5, which shows significant similarity to putative odorant receptors in A. gambiae and Drosophila melanogaster and which is selectively expressed in olfactory organs. AgOr5 is tightly clustered within the A. gambiae genome to two other highly homologous candidate odorant receptors, suggesting that these genes are derived from a common ancestor. Analysis of the developmental expression within members of this AgOR gene cluster reveals considerable variation between these AgORs as compared to candidate odorant receptors from D. melanogaster. 相似文献
54.
The effect of abscisic acid on growth, ultrastructure and nucleic acid biosynthesis was studied in tissue culture of spinach (Spinacia oleracea L.). Low concentration (0.01 mg l?1) of abscisic acid increased fresh and dry weight of calluses, whereas 1.0 mg l?1 was inhibitory. The stimulating effect was observed only in the presence of a relatively high concentration of kinetin (1 mg l?1). The inhibitory effect was partly overcome by the same kinetin concentration. The low concentration of abscisic acid probably accelerated the induction of callus growth after subculture and stimulated cell division in the exponential phase of growth. Electron microscopy showed the presence of numerous polysomes and rough endoplasmic reticulum in callus cells grown at the stimulating abscisic acid concentration. Control cells and cells at the inhibitory concentration had slightly hyaline cytoplasm and were more vacuolated. Incubation of callus tissue with 32P in the presence of stimulating concentration of abscisic acid showed a significant increase in the rate of biosynthesis of all nucleic acid classes after 8 h, whereas inhibitory concentration produced a decrease in 32P incorporation. However, when the tissue was grown in the presence of abscisic acid for 20 days, both concentrations decreased the rate of nucleic acid biosynthesis, as compared to the controls. 相似文献
55.
56.
IV Zlatkin M Schneider FJ de Bruijn LJ Forney PhD 《Journal of industrial microbiology & biotechnology》1996,17(3-4):219-227
Culturable bacteria from the deep subsurface (179 m) at Cerro Negro, New Mexico were isolated and characterized. The average number of viable aerobic bacteria was estimated to be 5×105g–1 of sediment, but only about 0.1% of these could be recovered on agar medium when incubated under aerobic conditions. Of 158 strains isolated from this depth, 92 were characterized by cellular fatty acid profiles (FAME), 36 by analysis of partial 16S rDNA sequences, and 44 by rep-PCR genome fingerprint analysis using three different sets of oligonucleotide primers (REP, BOX, or ERIC). These analyses showed the majority of isolates (67%) were Gram-positive bacteria and primarily members of genera with a high %G+C DNA. The remaining isolates were -subdivisionProteobacteria (19%) and members of the flavobacteria group (14%). The diversity indices based on these different methods of characterization were very high suggesting this subsurface habitat harbors a highly diverse microbial community. 相似文献
57.
58.
为建立鸭乙型肝炎病毒LJ-76的转染细胞系,将LJ-76病毒DNA插入到pUC19的EcoRⅠ位点上,分离得到含有双拷贝LJ-76DNA的重组质粒.通过磷酸钙沉淀方法,将经CsCl等密度离心纯化的LJ-76DNA双体导入到人肝癌细胞BEL7402中.收集转染细胞的培养液进行蔗糖密度梯度离心,所得沉淀经检测发现含有LJ-76DNA并具有特异性DHBV内源性DNA多聚酶活性;对上述样品通过DotEIA检测DHBV核心抗原及表面抗原结果为阳性.Southernblot分析表明转染细胞内存在病毒DNA复制中间体cccDNA、ssDNA和rcDNA,而cccDNA被认为是复制活动较为活跃的标志.电镜观察转染细胞的上清发现有病毒颗粒的存在. 相似文献
59.
Laurence J. Zwiebel Vivian H. Cohn Donna R. Wright Gordon P. Moore 《Journal of molecular evolution》1982,19(1):62-71
Summary Single-copy DNA was isolated fromDrosophila melanogaster and hybridized with total genomic DNA ofD. melanogaster, D. mauritiana, D. simulans, D. pseudoobscura, D. willistoni, D. hydei andD. virilis. The duplexes were thermally eluted from hydroxyapatite and the data used to assess the relatedness of each species toD. melanogaster. The general pattern of relatedness was similar to that predicted by morphological methods but with some notable exceptions. The rate of nucleotide substitution was estimated to be greater than 0.66% of bases per million years. An unexpected, rapidly evolving component ofD. melanogaster single-copy DNA was identified. The relatedness of these species was also studied with respect to the gene coding for alcohol dehydrogenase (ADH). The ADH gene, previously cloned fromD. melanogaster (Goldberg 1980), was hybridized with Southern blots of genomic digests of the seven species. The intensity and position of the hybridizing bands suggest the amount of divergence of the gene. Divergence was quantitated by reassociation of a fragment of the cloned ADH gene with total DNA of the seven drosophilids and thermal elution of the resultant duplexes from hydroxyapatite. The ADH gene was isolated from genomic clone libraries ofD. melanogaster, D. simulans andD. mauritiana and further studied by comparison of position of restriction sites. Species relationships deduced from comparison of total single-copy DNA and the ADH gene were consistent, demonstrating that a single gene can reflect divergence of the entire genome. 相似文献
60.
C. Elaine Merrill Tracy M. Sherertz William B. Walker L. J. Zwiebel 《Developmental neurobiology》2005,63(1):15-28
The ability to modulate olfactory sensitivity is necessary to detect chemical gradients and discriminate among a multitude of odor stimuli. Desensitization of odorant receptors has been postulated to occur when arrestins prevent the activation of downstream second messengers. A paucity of in vivo data on olfactory desensitization prompts use of Drosophila melanogaster genetics to investigate arrestins' role in regulating olfactory signaling pathways. Physiological analysis of peripheral olfactory sensitivity reveals decreased responsiveness to a host of chemically distinct odorants in flies deficient for arrestin1 (arr1), arrestin2 (arr2), or both. These phenotypes are manifest in odorant‐ and dose‐ dependent fashions. Additionally, mutants display altered adaptive properties under a prolonged exposure paradigm. Behaviorally, arr1 mutants are impaired in olfactory‐based orientation towards attractive odor sources. As the olfactory deficits vary according to chemical identity and concentration, they indicate that a spectrum of arrestin activity is essential for odor processing depending upon the particular olfactory pathway involved. Arrestin mutant phenotypes are hypothesized to be a consequence of down‐regulation of olfactory signaling to avoid cellular excitotoxicity. Importantly, phenotypic rescue of olfactory defects in arr11 mutants is achieved through transgenic expression of wild‐type arr1. Taken together, these data clearly indicate that arrestins are required in a stimulus‐specific manner for wild type olfactory function and add another level of complexity to peripheral odor coding mechanisms that ultimately impact olfactory behavior. © 2004 Wiley Periodicals, Inc. J Neurobiol, 2005 相似文献