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261.
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Hadas Hawlena Farrah Bashey Curtis M. Lively 《Evolution; international journal of organic evolution》2010,64(11):3198-3204
Spite occurs when an individual harms itself in the act of harming other individuals. Such behaviors were once assumed to be of limited evolutionary importance, as the conditions for the evolution of spite were thought to be too restrictive. Recent theoretical work, however, suggests that spatial population structure, which allows local competition among genotypes, could favor the evolution of spite. One of the clearest examples of spite is the costly production and release by bacteria of toxins (called bacteriocins) that can kill unrelated strains of the same species. Here, we establish the existence of spatial structure in two natural populations of bacteriocin‐producing bacteria. Specifically, relatedness decreased with increasing spatial distance between the field isolates. In addition, toxin‐mediated inhibitions were found only between isolates that were collected more than 1 m apart and that were generally less than 80% similar in their genomic fingerprints. Taken together, the results suggest that the bacteria are spatially structured, with mixing of genotypes and spiteful interactions at the boundaries between demes. 相似文献
264.
Ewa Dubas Gabriela Golebiowska Iwona Zur Maria Wedzony 《Acta Physiologiae Plantarum》2011,33(2):529-537
According to regular reports, one of the most serious diseases of winter cereal and grass varieties in moderate and cold climatic
areas is pink snow mould caused by Microdochium nivale. Currently, the resistance of the economically important cereal species as triticale is not satisfactory. Moreover, there
is no efficient strategy of protection against this pathogen and the understanding of plant resistance mechanisms is rather
poor. Presented paper for the first time shows the cytological analysis of M. nivale infection in model triticale varieties by the use of fluorescent and light microscopy in combination with fluorescent dyes
and hydrogen peroxide staining. Both, the infection level and the dynamic of the process varied for tested genotypes confirming
the field and laboratory data of their different resistance to this pathogen. Moreover, our analysis showed that in both cultivars
cold-hardening of seedlings delayed the mycelium growth. In both cultivars, hyphal walls and fungal penetration sites were
visualized in crowns, leaf sheaths and leaves of hardened and non-hardened inoculated seedlings. For the first time the presence
of the haustoria produced by M. nivale was confirmed in those tissues. Single infection hyphae usually penetrated into the host tissues via stomatal apparatuses
were accompanied by the efflux of hydrogen peroxide. The data show a great potential of fluorescence techniques in studying
the host plant–pathogen interactions providing a better insight into plant defence reactions that may allow elaboration of
the efficient breeding strategies aimed at increasing resistance to this pathogenic fungus. 相似文献
265.
A strain of Coleps hirtus (Ciliophora, Prorodontida) was isolatedfrom the epilimnion of monomictic Lake Kinneret. Growth of thisciliate was tested in response to 12 species of planktonic algaeand seven species of cultured bacteria from lake isolates whichwere offered as food. Eight species of algae (one Cryptophyceaeand seven Chlorophyceae) and four bacteria supported good toexcellent growth of C.hirtus. Growth rates (µ) and doublingtimes (DT) ranged from 0.008 to 0.029 h1 and from 23.9to 90.8 h respectively. C.hirtus was able to grow on bacteriaat concentration levels as low as 28 x 105 cells ml1.No correlation was observed between growth rate of C.hirtusand cell volume of the prey.
aPresent address: Istituto di Ecologia, Universita di Parma,43100 Parma, Italy 相似文献
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This study is part of an ongoing attempt to identify and characterize proteins associated with the human decidual tissue. A novel decidual-associated glycoprotein with an apparent molecular weight of 71 kD named hDP71 (human decidual-protein 71), has been identified and purified by immunoaffinity technique using monoclonal antibodies. The monoclonal antibodies recognizing the hDP71 were raised against a partly purified preparation of decidual associated proteins, which was obtained by immunoabsorption of serum proteins from crude decidual extract. Although the hDP71 was copurified with another decidual-associated glycoprotein, the previously described hDP200 (Halperin et al., 1989), evidence is presented showing that the monoclonal antibodies described above are specific for hDP71. 相似文献
268.
The M2 protein is an essential component of the Influenza virus’ infectivity cycle. It is a homo-tetrameric bundle forming
a pH-gated H+ channel. The structure of M2 was solved by three different groups, using different techniques, protein sequences and pH environment.
For example, solid-state NMR spectroscopy was used on a protein in lipid bilayers, while X-ray crystallography and solution
NMR spectroscopy were applied on a protein in detergent micelles. The resulting structures from the above efforts are rather
distinct. Herein, we examine the different structures under uniform conditions such as a lipid bilayer and specified protonation
state. We employ extensive molecular dynamics simulations, in several protonation states, representing both closed and open
forms of the channel. Exploring the properties of each of these structures has shown that the X-ray structure is more stable
than the other structures according to various criteria, although its water conductance and water-wire formation do not correlate
to the protonation state of the channel. 相似文献
269.
Bavat Bornstein Hagar Meltzer Ruth Adler Idan Alyagor Victoria Berkun Gideon Cummings Fabienne Reh Hadas KerenShaul Eyal David Thomas Riemensperger Oren Schuldiner 《The EMBO journal》2021,40(12)
The mechanisms controlling wiring of neuronal networks are not completely understood. The stereotypic architecture of the Drosophila mushroom body (MB) offers a unique system to study circuit assembly. The adult medial MB γ‐lobe is comprised of a long bundle of axons that wire with specific modulatory and output neurons in a tiled manner, defining five distinct zones. We found that the immunoglobulin superfamily protein Dpr12 is cell‐autonomously required in γ‐neurons for their developmental regrowth into the distal γ4/5 zones, where both Dpr12 and its interacting protein, DIP‐δ, are enriched. DIP‐δ functions in a subset of dopaminergic neurons that wire with γ‐neurons within the γ4/5 zone. During metamorphosis, these dopaminergic projections arrive to the γ4/5 zone prior to γ‐axons, suggesting that γ‐axons extend through a prepatterned region. Thus, Dpr12/DIP‐δ transneuronal interaction is required for γ4/5 zone formation. Our study sheds light onto molecular and cellular mechanisms underlying circuit formation within subcellular resolution. 相似文献
270.
Specific binding of human alpha interferon to a high affinity cell surface binding site on bovine kidney cells 总被引:11,自引:0,他引:11
Virus-induced human alpha interferon (HuIFN-alpha) derived from Namalwa cells and purified to a specific activity of 2 X 10(8) units/mg of protein was radiolabeled with 125I-labeled Bolton and Hunter reagent to a specific activity of 4-12 microCi/micrograms of protein. The binding of this 125I-IFN to bovine kidney cells was examined at 4 degrees C. Scatchard analysis of the binding data indicate the presence of 650 binding sites/cell and binding of the ligand with an apparent Kd of 6 X 10(-11) M. Trypsin or acid treatment of cells to which 125I-IFN was bound resulted in the release of greater than or equal to 77% of the radioactivity, indicating a majority of radiolabeled material was bound to the cell surface. Antibodies against human leukocyte IFN but not antibodies against human fibroblast IFN inhibited the binding of radiolabeled IFN to the cells. The binding of 125I-IFN was not inhibited by a 75-fold molar excess of mouse IFN but was inhibited 30% by a 200-fold molar excess of human beta (fibroblast) IFN. These data are compatible with the Lower biological activities of these IFNs on bovine kidney cells. Several Escherichia coli derived HuIFN-alpha s inhibited the binding of the radiolabeled IFN to the same extent as native HuIFN-alpha s, but four fragments of HuIFN-alpha 1, an E. coli-derived 86 amino acid NH2-terminal fragment as well as 3 different synthetic carboxy-terminal fragments of 140, 56, or 46 amino acids did not inhibit binding. 相似文献