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Zhaomin Li Hui Peng Li Qin Jing Qi Xiaobing Zuo Jing-Yuan Liu Jian-Ting Zhang 《The Journal of biological chemistry》2013,288(44):31447-31457
Many proteins exist and function as homodimers. Understanding the detailed mechanism driving the homodimerization is important and will impact future studies targeting the “undruggable” oncogenic protein dimers. In this study, we used 14-3-3σ as a model homodimeric protein and performed a systematic investigation of the potential roles of amino acid residues in the interface for homodimerization. Unlike other members of the conserved 14-3-3 protein family, 14-3-3σ prefers to form a homodimer with two subareas in the dimeric interface that has 180° symmetry. We found that both subareas of the dimeric interface are required to maintain full dimerization activity. Although the interfacial hydrophobic core residues Leu12 and Tyr84 play important roles in 14-3-3σ dimerization, the non-core residue Phe25 appears to be more important in controlling 14-3-3σ dimerization activity. Interestingly, a similar non-core residue (Val81) is less important than Phe25 in contributing to 14-3-3σ dimerization. Furthermore, dissociating dimeric 14-3-3σ into monomers by mutating the Leu12, Phe25, or Tyr84 dimerization residue individually diminished the function of 14-3-3σ in resisting drug-induced apoptosis and in arresting cells at G2/M phase in response to DNA-damaging treatment. Thus, dimerization appears to be required for the function of 14-3-3σ. 相似文献
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维甲酸X受体α(Retinoid X receptor-α,RXR-α)属于核受体家族,在调节基因转录及信号转导方面发挥着重要的作用。为了深入研究RXR-α的生物学作用,文章利用RT-PCR技术扩增了人RXR-α基因,并将其克隆入原核表达载体pQE-30Xa,转化大肠杆菌M15[PREP4],异丙基硫代-β-D-半乳糖苷(IPTG)诱导表达,SDS-PAGE可见与预期大小相符的蛋白条带,Western blotting证实该条带为重组人RXR-α蛋白,并用Ni2-NTA柱进行亲和层析纯化。免疫共沉淀结果显示其具有与TRβ1结合的能力。电泳迁移率改变实验(EMSA)显示RXR-α与TRβ1形成的杂二聚体具有与DNA结合的能力。结果显示,文章已成功建立了重组人RXR-α的大肠杆菌表达系统,表达产物具有很好的生物学活性。 相似文献
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原生质体电融合酵母多倍体生理特性的研究 总被引:5,自引:0,他引:5
对原生质体电融合技术获得的11株酵母多倍体融合株,进行了一系列生理特性的分析比较.结果发现,在电融合过程中,融合株的细胞体积及DNA含量并不随着核倍性呈线性递增.而是有其特殊的变化规律。当糖化酵母sta1、sta2和sta3在细胞核中各自纯合时,明显表现出表达的剂量效应。其中sta1、sta2纯合的融合株.在YEPS培养中,GA分泌可被淀粉大量诱导,表现出一定的二次生长特性。从分析结果推测,在亲株5301-14D及HU-TY—1A中可能有对GA分泌不利的因素。 相似文献
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Iron (Fe) is an essential and important trace element for animals. In order to study its metabolism and relationship with hepcidin, piglet models of Fe-deficiency and Fe-overload were established by intramuscular injection with different doses of Fe-dextran (150 mg Fe/ml) within 1 week of age. Twelve piglets were divided into three groups of four animals: deficiency, regular and overload group, receiving 0 ml, 1 ml and 6 ml Fe-dextran, respectively. The piglets were euthanised at the age of 7 days for analysis. The results showed that the Fe-concentrations in liver, spleen and serum of piglets in the overload group were higher than in the regular and deficiency groups (p < 0.05). In the overload group, several serum biochemical parameters, e.g. globulin, total bilirubin, total cholesterol, high density lipoprotein (HDL), malondialdehyde, glutathione peroxidase (GPx), peroxidase and xanthine oxidase were higher, while alkaline phosphatase (AKP) and triglycerides were lower, compared with the regular group (p < 0.05). The serum concentrations of AKP, total bilirubin and peroxidase in the deficiency group were lower, while HDL and GPx were higher, compared with the regular group (p < 0.05). Hepcidin mRNA abundance was 131 times lower in the liver of piglets with Fe-deficiency, and 7 times higher in the overloaded group than that in the regular group (p < 0.05). In conclusion, Fe-overload and deficiency would influence Fe-metabolism, serum biochemical indexes, oxidation state and hepcidin mRNA abundance in piglet liver. 相似文献
89.
S-亚硝基化是一种重要的蛋白质翻译后修饰方式, 是指一氧化氮(NO)基团共价连接至靶蛋白特定半胱氨酸残基的自由巯基, 从而形成S-亚硝基硫醇(SNO)的过程。S-亚硝基化修饰广泛存在于各有机体中, 通过改变蛋白质生化活性、稳定性、亚细胞定位以及蛋白质-蛋白质相互作用等机制而调控不同的生物学过程或信号通路。在蛋白质S-亚硝基化检测分析方法中, 最为广泛使用的是生物素转化法(biotin switch assay), 其基本原理是首先封闭未被修饰的自由巯基, 进而将被修饰的SNO基团特异地还原为自由巯基并使用生物素将其特异标记。被生物素标记的半胱氨酸残基(即被修饰位点)可进一步通过蛋白质免疫印迹和/或质谱等方法进行检测分析。该文详细描述了植物蛋白质样品的体内和体外生物素转化法的实验流程, 并对实验过程中的注意事项进行了讨论。 相似文献
90.
Mapping Nonfullerene Acceptors with a Novel Wide Bandgap Polymer for High Performance Polymer Solar Cells 下载免费PDF全文
Xunfan Liao Zhaoyang Yao Ke Gao Xueliang Shi Lijian Zuo Zonglong Zhu Lie Chen Feng Liu Yiwang Chen Alex K.‐Y. Jen 《Liver Transplantation》2018,8(24)
A new weak electron‐deficient building block, bis(2‐ethylhexyl) 2,5‐bis(5‐bromothiophen‐2‐yl) thieno[3,2‐b]thiophene‐3,6‐dicarboxylate ( TT‐Th ), is incorporated to construct a wide‐bandgap (1.88 eV) polymer PBDT‐TT for nonfullerene polymer solar cells (NF‐PSCs). PBDT‐TT possesses suitable energy levels and complementary absorption when blended with both ITIC analogues ( ITIC and IT‐M ) and a near‐infrared (NIR) acceptor ( 6TIC ). Moreover, PBDT‐TT exhibits good conjugated planarity and preferable face‐on orientation in the blended thin film, which are beneficial for charge transfer and carrier transport. The PSCs based on PBDT‐TT : IT‐M and PBDT‐TT : 6TIC blend films yield high power conversion efficiencies of 11.38% and 11.03%, respectively. To the best of the authors' knowledge, the PCE of 11.03% for PBDT‐TT : 6TIC‐ based device is one of the highest values reported for NIR NF‐PSCs. This work demonstrates that TT‐Th is a useful new electron‐accepting building block for making p‐type wide bandgap polymers for efficient NIR NF‐PSCs. 相似文献