yeaI基因对奶牛源大肠杆菌NJ17生物学特性的影响

c-di-GMP是细菌中广泛存在的第二信使,可通过效应蛋白参与调控细菌的生物被膜形成、运动性和毒力等生物学特性。YeaI因含有能结合c-di-GMP分子的EGEVF基序,可能作为c-di-GMP效应蛋白发挥作用。[目的] 研究yeaI基因缺失对奶牛源大肠杆菌临床分离株NJ17生物学特性的影响。[方法] 构建NJ17的yeaI缺失株（NJ17ΔyeaI）及回复株cNJ17ΔyeaI,分析yeaI对NJ17生物学特性（如生长特性、生物被膜形成能力和对小鼠乳腺上皮细胞（EpH₄-Ev）的黏附）的影响。[结果] 成功构建NJ17的yeaI缺失株（NJ17ΔyeaI）及其回复株（cNJ17ΔyeaI）;与野生株NJ17相比,缺失株NJ17ΔyeaI生长特性及耐药性无显著变化,生物被膜形成能力显著下降,运动性显著升高（P<0.05）;透射电镜检测结果表明,yeaI缺失影响NJ17菌毛和鞭毛的形成;实时定量PCR（qPCR）结果显示,yeaI基因显著抑制NJ17鞭毛基因filG和motB的转录水平（P<0.05）;血清杀菌实验表明,yeaI缺失能显著增强其抵抗血清杀菌作用（P<0.05）;对EpH₄-Ev细胞黏附实验表明,yeaI缺失对NJ17黏附性无显著影响（P>0.05）。[结论] yeaI对奶牛源大肠杆菌NJ17的生物学特性具有重要的调控作用。     

Plant functional diversity enhances associations of soil fungal diversity with vegetation and soil in the restoration of semiarid sandy grassland

The trait‐based approach shows that plant functional diversity strongly affects ecosystem properties. However, few empirical studies show the relationship between soil fungal diversity and plant functional diversity in natural ecosystems. We investigated soil fungal diversity along a restoration gradient of sandy grassland (mobile dune, semifixed dune, fixed dune, and grassland) in Horqin Sand Land, northern China, using the denaturing gradient gel electrophoresis of 18S rRNA and gene sequencing. We also examined associations of soil fungal diversity with plant functional diversity reflected by the dominant species' traits in community (community‐weighted mean, CWM) and the dispersion of functional trait values (FD_is). We further used the structure equation model (SEM) to evaluate how plant richness, biomass, functional diversity, and soil properties affect soil fungal diversity in sandy grassland restoration. Soil fungal richness in mobile dune and semifixed dune was markedly lower than those of fixed dune and grassland (P < 0.05). Soil fungal richness was positively associated with plant richness, biomass, CWM plant height, and soil gradient aggregated from the principal component analysis, but SEM results showed that plant richness and CWM plant height determined by soil properties were the main factors exerting direct effects. Soil gradient increased fungal richness through indirect effect on vegetation rather than direct effect. The negative indirect effect of FDis on soil fungal richness was through its effect on plant biomass. Our final SEM model based on plant functional diversity explained nearly 70% variances of soil fungal richness. Strong association of soil fungal richness with the dominant species in the community supported the mass ratio hypothesis. Our results clearly highlight the role of plant functional diversity in enhancing associations of soil fungal diversity with community structure and soil properties in sandy grassland ecosystems.     

Cerebral Metabolic Differences Associated with Cognitive Impairment in Parkinson’s Disease

PurposeTo characterize cerebral glucose metabolism associated with different cognitive states in Parkinson’s disease (PD) using ¹⁸F-fluorodeoxyglucose (FDG) and Positron Emission Tomography (PET).MethodsThree groups of patients were recruited in this study including PD patients with dementia (PDD; n = 10), with mild cognitive impairment (PD-MCI; n = 20), and with no cognitive impairment (PD-NC; n = 30). The groups were matched for age, sex, education, disease duration, motor disability, levodopa equivalent dose and Geriatric Depression Rating Scale (GDS) score. All subjects underwent a FDG-PET study. Maps of regional metabolism in the three groups were compared using statistical parametric mapping (SPM5).ResultsPD-MCI patients exhibited limited areas of hypometabolism in the frontal, temporal and parahippocampal gyrus compared with the PD-NC patients (p < 0.01). PDD patients had bilateral areas of hypometabolism in the frontal and posterior parietal-occipital lobes compared with PD-MCI patients (p < 0.01), and exhibited greater metabolic reductions in comparison with PD-NC patients (p < 0.01).ConclusionsCompared with PD-NC patients, hypometabolism was much higher in the PDD patients than in PD-MCI patients, mainly in the posterior cortical areas. The result might suggest an association between posterior cortical hypometabolism and more severe cognitive impairment. PD-MCI might be important for early targeted therapeutic intervention and disease modification.     

Root decomposition of <Emphasis Type="Italic">Artemisia halodendron</Emphasis> and its effect on soil nitrogen and soil organic carbon in the Horqin Sandy Land,northeastern China

Root decomposition is a critical feedback from the plant to the soil, especially in sandy land where strong winds remove aboveground litter. As a pioneer shrub in semi-mobile dunes of the Horqin sandy land, Artemisia halodendron has multiple effects on nutrient capture and the microenvironment. However, its root decomposition has not been studied in terms of its influence on soil organic carbon (SOC) and nitrogen (N). In this study, we buried fine (≤2 mm) and coarse roots in litterbags at a depth of 15 cm below semi-mobile dunes. We measured the masses remaining and the C and N contents at intervals during 434 days of decomposition. The soils below the litterbags were then divided into layers and sampled to measure the SOC and N contents. After rapid initial decomposition, both coarse and fine roots decomposed slowly. After 53 days, 36.2 % of coarse roots and 39.8 % of fine roots had decomposed. In contrast, only 18.4 % of coarse roots and 30.5 % of fine roots decomposed in the following 381 days. Fine roots decomposed significantly faster, and their decomposition rate after the initial rapid decay was strongly related to climate (R ² = 0.716, P < 0.05). Root decomposition increased SOC and N contents below the litterbags, with larger effects for fine roots. The SOC content was more variable between soil layers than the N content. Thus, decomposition of A. halodendron roots cannot be ignored when studying SOC and N feedbacks from plants to the soil, particularly for fine roots.     

Regulation of mitochondrial NAD pool via NAD+ transporter 2 is essential for matrix NADH homeostasis and ROS production in Arabidopsis

Reactive oxygen species(ROS) play a crucial role in numerous biological processes in plants, including development, responses to environmental stimuli, and programmed cell death(PCD). Deficiency in MOSAIC DEATH 1(MOD1), a plastid-localized enoyl-ACP reductase essential for de novo fatty acid biosynthesis in Arabidopsis thaliana, leads to the increased malate export from chloroplasts to mitochondria, and the subsequent accumulation of mitochondria-generated ROS and PCD. In this study, we report the identification and characterization of a mod1 suppressor, som592. SOM592 encodes mitochondrion-localized NAD~+ transporter 2(NDT2). We show that the mitochondrial NAD pool is elevated in the mod1 mutant. The som592 mutation fully suppressed mitochondrial NADH hyper-accumulation, ROS production, and PCD in the mod1 mutant, indicating a causal relationship between mitochondrial NAD accumulation and ROS/PCD phenotypes. We also show that in wild-type plants, the mitochondrial NAD+uptake is involved in the regulation of ROS production in response to continuous photoperiod. Elevation of the alternative respiration pathway can suppress ROS accumulation and PCD in mod1, but leads to growth restriction. These findings uncover a regulatory mechanism for mitochondrial ROS production via NADH homeostasis in Arabidopsis thaliana that is likely important for growth regulation in response to altered photoperiod.     

A Desolvated Solid–Solid Interface for a High‐Capacitance Electric Double Layer

Understanding the electric double layer is essential for achieving efficient electrochemical energy storage technologies. A conventional solid–liquid electrode interface suffers from serious self‐discharge and a narrow voltage window, which makes the development of a solid–solid interface imperative. However, an in‐depth understanding of the electric double layer with a solid–solid interface is lacking. Here, a solid–solid interfacial electric double layer is proposed with excellent electrochemical performance. The solid layer is constructed by the electrochemical decomposition of lithium difluoro(oxalate)borate, which provides a desolvated environment for the establishment of a electric double layer. This makes a stronger interaction between the electrode surface and the ions. Based on this unique property, it is found that the solid–solid interfacial electric double layer has an increased capacitance, which suggests a way to develop high‐energy electrochemical capacitors.