Synthesis of polymerizable phospholipid derivatives and creation of polymer monolayers and liposomes with immobilized bacteriorhodopsin

Polymerizable phospholipid derivatives with N-acryloyl groups have been synthesised; monomeric and polymeric monolayers and liposomes based on these compounds have been prepared and investigated. Bacteriorhodopsin immobilized in the monomeric and polymeric liposomes was shown to retain its native activity.     

Hybridization analysis of nucleic acids using stained latex]

Method of the latex hybridization analysis has been developed: after hybridization of NA-target with biotinylated probe visualization of hybrids was carried out using latex particles, containing fluorescent dye pyronin G and coated with streptavidin. Due to encapsulation of the fluorescent dye in polymer particles sensitivity of the analysis was increased by several orders of magnitude in comparison with methods, using fluorescently labelled probes. Possessing a number of advantages, the method yields to none of any other methods of NA hybridization analysis in sensitivity.     

The action of blood serum and its components on potential-dependent sodium channels in neuroblastoma cells.

Blood plasma, serum and its fractions containing components of different molecular weights as well as some identified serum constituents were tested for their action on sodium currents of voltage-clamped, internally dialyzed neuroblastoma cells. Only components with a molecular weight over 50 kDa produced a persistent increase in sodium channel currents (stimulatory effect) and shifts in activation and inactivation curves along the voltage axis towards more negative or positive potentials, respectively (modifying effect). Both modulations taken together provide a somewhat higher level of sodium electro-excitable system activity. Among the identified serum components tested, including those possessing high physiological activity, albumin was the only one which reproduced the effects of whole blood serum both qualitatively and quantitatively. The data obtained allow to assume albumin to play a role of an active substance responsible for the blood plasma or serum effects on the potential-dependent transporting system in the neuroblastoma cell membrane. Albumin seems to be involved in holding the functional activity of sodium channels on a suitable level rather than being involved in any regulatory processes.