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The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber
formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides
in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs. 相似文献
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Activation tagging identifies a gene from Petunia hybrida responsible for the production of active cytokinins in plants 总被引:16,自引:0,他引:16
Zubko E Adams CJ Macháèková I Malbeck J Scollan C Meyer P 《The Plant journal : for cell and molecular biology》2002,29(6):797-808
Cytokinins (CKs) are phytohormones that play an important role in plant growth and development. Although the first naturally produced CK, zeatin, was isolated almost four decades ago, no endogenous gene has been shown to produce active CKs in planta. In an activation tagging experiment we have identified a petunia line that showed CK-specific effects including enhanced shooting, reduced apical dominance and delayed senescence and flowering. This phenotype correlated with the enhanced expression of a gene we labelled Sho (Shooting). Sho, which encodes a protein with homology to isopentenyl transferases (IPTs), also causes CK-specific effects when expressed in other plant species. In contrast to the ipt gene from Agrobacterium, which primarily increases zeatin levels, Sho expression in petunia and tobacco especially enhances the levels of certain N6-(delta2-isopentenyl) adenosine (2iP) derivatives. Our data suggest that Sho encodes a plant enzyme whose activity is sufficient to produce active CKs in plants. 相似文献
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Zubko MK Zubko EI Ruban AV Adler K Mock HP Misera S Gleba YY Grimm B 《The Plant journal : for cell and molecular biology》2001,25(6):627-639
The genetic basis of multiple phenotypic alterations was studied in cell-engineered cybrids Nicotiana tabacum (+ Hyoscyamus niger) combining the nuclear genome of N. tabacum, plastome of H. niger and recombinant mitochondria. The plants possess a complex, maternally inheritable syndrome of nucleo-cytoplasmic incompatibility, severely affecting growth, metabolism and development. In vivo, the syndrome was manifested as: late germination of seeds; dramatic decrease of chlorophyll and carotenoids in cotyledons and leaves; altered morphology of cotyledons, leaves and flowers; and dwarfism. The leaf phenotype depended on light intensity. In 'green flowers' (an extreme phenotype), homeotic function B was downregulated. In vitro, the incompatibility syndrome was restricted to the pigment deficiency of cotyledons. Electron microscopy revealed perturbations in the differentiation of chloroplasts and palisade parenchyma cells in bleached leaves. The pigment deficiency accompanied by retarded growth is discussed as a result of plastome-genome incompatibility, whereas other features are likely to be due to nucleo-mitochondrial incompatibilities. 相似文献
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Urwin Peter E. Troth Kevin M. Zubko Elena I. Atkinson Howard J. 《Molecular breeding : new strategies in plant improvement》2001,8(1):95-101
A cysteine proteinase inhibitor expressed in potato plants provides the first demonstration that transgenic resistance to nematodes such as the potato cyst nematode Globodera pallida can be effective under field conditions. The highest level of resistance obtained in the field for one of the four transformed lines of the normally fully susceptible Solanum tuberosum tuberosum cv. Désirée was 70±9%. The partially resistant cv. Sante that is currently of commercial use in the UK showed a resistance of 85±3%. In containment experiments a change in G. pallida population to one known to be virulent against cv. Sante caused a significant loss of its resistance from 80±4% to only 51±6%. In contrast, the resistance of transgenic Désirée was similar for challenge by the avirulent and virulent populations with values of 68±6% and 71±4%, respectively. Constitutive expression of the cystatin had no detrimental effect on either number or weight of tubers and the weight of the haulm for 3 of the 4 lines in the field. The results establish that transgenic field resistance against G. pallida can be achieved. Clearly food and environmental safety must be assured before commercialisation can be contemplated. However, a prima facie case can be made that the technology is benign and can reduce the use of environmentally hazardous nematicides. 相似文献
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Kovalevskiy A. V. Zubko K. S. Efimova A. R. Luchnikova E. M. Drozdova O. M. 《Entomological Review》2019,99(1):135-135
Entomological Review - The figure on p. 1381 should be as follows: 相似文献