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591.
Zsuzsanna Izsvák Perry B. Hackett Laurence J.N. Cooper Zoltán Ivics 《BioEssays : news and reviews in molecular, cellular and developmental biology》2010,32(9):756-767
Recent results confirm that long‐term expression of therapeutic transgenes can be achieved by using a transposon‐based system in primary stem cells and in vivo. Transposable elements are natural DNA transfer vehicles that are capable of efficient genomic insertion. The latest generation, Sleeping Beauty transposon‐based hyperactive vector (SB100X), is able to address the basic problem of non‐viral approaches – that is, low efficiency of stable gene transfer. The combination of transposon‐based non‐viral gene transfer with the latest improvements of non‐viral delivery techniques could provide a long‐term therapeutic effect without compromising biosafety. The new challenges of pre‐clinical research will focus on further refinement of the technology in large animal models and improving the safety profile of SB vectors by target‐selected transgene integration into genomic “safe harbors.” The first clinical application of the SB system will help to validate the safety of this approach. 相似文献
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András Seregi Péter Serfözö Zsuzsanna Mergl rás Schaefer 《Journal of neurochemistry》1982,38(1):20-27
Abstract: The mechanism of involvement of monoamine oxidase (MAO) in catecholamine-stimulated prostaglandin (PG) biosynthesis was studied in the particulate fraction of rat brain homogenates. High concentrations of either noradrenaline (NA) or dopamine (DA) stimulated effectively PGF2α formation. The same amount of 2-phenylethylamine (PEA) acted similarly, provided that it was administered together with a catecholamine analogue or metabolite possessing the 3,4-dihydroxyphenyl nucleus–3, 4-dihydroxyphenylalanine (DOPA), 3,4-dihydroxyphenylacetic acid (DOPAC), 3,4-dihydroxyphenyl-glycol (DOPEG), 3,4-dihydroxyphenylacetaldehyde (DOPAL), or α-methylnoradrenaline (α-met-NA)–or with SnCl2 . In the absence of PEA, these compounds were ineffective with regard to stimulation of PGF2α formation. Catalase, pargyline, or indomethacin abolished completely PGF2α formation elicited either by catecholamines or by PEA plus a 3,4-dihydroxyphenyl compound or SnCl2 . With regard to the stimulation of PGF2α formation in the presence of α-met-NA, PEA could be replaced by H2 O2 , generated by the glucose oxidase(GOD)-glucose system. The effect of H2 O2 was inhibited by indomethacin or catalase, but pargyline was ineffective. It is assumed that catecholamines play a dual role in the activation of PG biosynthesis in brain tissue. During the enzymatic decomposition of catecholamines MAO produces H2 O2 , which stimulates endoperoxide synthesis. Simultaneously, catecholamines as hydrogen donors promote the nonenzymatic transformation of endoperoxides into PGF2α . The possible physiological importance of these findings is discussed. 相似文献
594.
Beatrix M Horvath Ben Scheres Beatrix M Horvath Hana Kourova Szilvia Nagy Edit Nemeth Zoltan Magyar Csaba Papdi Zaki Ahmad Gabino F Sanchez-Perez Serena Perilli Ikram Blilou Aladár Pettkó-Szandtner Zsuzsanna Darula Tamas Meszaros Pavla Binarova Laszlo Bogre Ben Scheres 《The EMBO journal》2017,36(9):1261-1278
595.
Katarzyna Parys Nicholas R. Colaianni Ho-Seok Lee Ulrich Hohmann Natalie Edelbacher Alen Trgovcevic Zuzana Blahovska Duhwa Lee Alexander Mechtler Zsuzsanna Muhari-Portik Mathias Madalinski Niklas Schandry Isaac Rodríguez-Arévalo Claude Becker Elisabeth Sonnleitner Arthur Korte Udo Bläsi Niko Geldner Youssef Belkhadir 《Cell host & microbe》2021,29(4):620-634.e9
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596.
597.
Molecular analysis of the waxy locus of Zea mays 总被引:1,自引:0,他引:1
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Zsuzsanna Szatmári Viktor Kis Mónika Lippai Krisztina Heged?s Tamás Faragó Péter L?rincz Tsubasa Tanaka Gábor Juhász Miklós Sass 《Molecular biology of the cell》2014,25(4):522-531
During autophagy, double-membrane autophagosomes deliver sequestered cytoplasmic content to late endosomes and lysosomes for degradation. The molecular mechanism of autophagosome maturation is still poorly characterized. The small GTPase Rab11 regulates endosomal traffic and is thought to function at the level of recycling endosomes. We show that loss of Rab11 leads to accumulation of autophagosomes and late endosomes in Drosophila melanogaster. Rab11 translocates from recycling endosomes to autophagosomes in response to autophagy induction and physically interacts with Hook, a negative regulator of endosome maturation. Hook anchors endosomes to microtubules, and we show that Rab11 facilitates the fusion of endosomes and autophagosomes by removing Hook from mature late endosomes and inhibiting its homodimerization. Thus induction of autophagy appears to promote autophagic flux by increased convergence with the endosomal pathway. 相似文献
600.
Vanessa Schegg Monique Vogel Svetlana Didichenko Michael B Stadler Zsuzsanna Beleznay Stephan Gadola Christine Sengupta Beda M Stadler Sylvia M Miescher 《Biologicals》2008,36(4):213-222
Inhibitory anti-muscarinic receptor type 3 (M3R) antibodies may contribute to the pathogenesis of Sj?gren's syndrome (SS), and putative anti-M3R blocking antibodies in intravenous immunoglobulin (IVIg) have been suggested as a rationale for treatment with IVIg. We investigated the presence of subtype-specific anti-MR autoantibodies in healthy donor and SS sera using MR-transfected whole-cell binding assays as well as M1R and M3R peptide ELISAs. Control antibodies against the second extracellular loop of the M3R, a suggested target epitope, were induced in rabbits and found to be cross-reactive on the peptides M3R and M1R. The rabbit antibodies had neither an agonistic nor an antagonistic effect on M3R-dependent ERK1/2 signalling. Only one primary SS (out of 5 primary SS, 2 secondary SS and 5 control sera) reacted strongly with M3R transfected cells. The same SS serum also reacted strongly with M1R and M2R transfectants, as well as M1R and two different M3R peptides. Strong binding to M1R and low-level activities against M3R peptides were observed both in SS and control sera. IVIg showed a strong reactivity against all three peptides, especially M1R. Our results indicate that certain SS individuals may have antibodies against M1R, M2R and M3R. Our results also suggest that neither the linear M3R peptide nor M3R transfectants represent suitable tools for discrimination of pathogenic from natural autoantibodies in SS. 相似文献