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581.
Krisztián?Kvell Tamás?Cz?mp?ly László?Hiripi Péter?Balogh József?Kóbor Lilla?Bodrogi Judit?E.?Pongrácz William?A.?Ritchie Zsuzsanna?B?szeEmail author 《Transgenic research》2010,19(1):105-112
Lentiviral technology is a powerful tool for the creation of stable transgenic animals. However, uncertainties have remained
whether constitutive promoters resist long-term silencing. We used concentrated HIV-1 based lentiviral vectors to create stable
transgenic BALB/c mice by perivitelline injection. In our vectors eGFP expression was driven by the human EF1α promoter. The
established transgenic animals were analyzed for eGFP expression by in vivo fluorescence imaging, PCR, histology and flow-cytometry.
eGFP expression showed even distribution without mosaicism; however, tissue-dependent differences of eGFP expression were
observed. Up to the sixth generation only one newborn showed eGFP inactivation. eGFP + transgenic bone marrow cells efficiently
provided long-term haemopoietic repopulation in radiation chimeras, regenerating all bone marrow-derived lineages with eGFP + cells
with distinct eGFP expression profiles. The established eGFP + BALB/c mouse strain is expected to be extremely useful in various
immunological experiments. 相似文献
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Institóris L Kovács D Kecskeméti-Kovács I Lukács A Szabó A Lengyel Z Papp A Nagymajtényi L Dési I 《Acta biologica Hungarica》2006,57(4):433-439
Detectable interactions between NOEL (No Observed Effect Level) doses of Pb, Hg and Cd in general toxicological, hematological, and immune function parameters were investigated. The metals (Pb-acetate, 20 mg/kg; HgCl2, 0.40 mg/kg; CdCl2, 1.61 mg/kg) were combined. First, the rats received the combination Pb + Hg + Cd for 4 weeks per os. Significant difference vs. control was found only in the weight of lung and popliteal lymph node (PLN). The Pb + Hg and Pb + Cd combinations significantly decreased the PLN to 100 g body weight and PLN to brain weight ratio, and Pb+Hg also decreased the relative adrenal weight. After 12 weeks treatment with the same doses, effects on the thymus, kidney, and adrenal weights in the Pb + Hg, and thymus weight in the Pb + Cd, combination were seen. Pb + Cd also affected the white and red blood cell count and hematocrit. Combined with Hg or Cd, NOEL dose Pb showed toxicity, indicating that exposure limits may be inefficient in combined exposure situations. 相似文献
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Ye X Kuklenyik Z Bishop AM Needham LL Calafat AM 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2006,844(1):53-59
Parabens (alkyl esters of p-hydroxybenzoic acid) are widely used as antimicrobial preservatives in cosmetic products, pharmaceuticals, and food processing. However, weak estrogenicity of some parabens has been revealed from several studies. Human exposure to parabens may be assessed by measuring the conjugated or free species of these compounds or their metabolites in urine. We have developed a method using on-line solid phase extraction-high performance liquid chromatography-isotope dilution tandem mass spectrometry with peak focusing to measure the urinary concentrations of methyl, ethyl, propyl, n- and iso- butyl, and benzyl parabens. This method has good reproducibility and accuracy with detection limits for all analytes below 0.2ng/mL in 100microL of urine, and permits quick and accurate analysis of a large number of samples in epidemiologic studies for assessing the prevalence of human exposure to parabens. Using this method, we detected methyl, ethyl, and propyl parabens, mostly as conjugated species, in 22 urine samples collected from anonymous adults. 相似文献
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Michele Wan Krisztina Hejjas Zsolt Ronai Zsuzsanna Elek Maria Sasvari‐Szekely Frances A. Champagne Ádám Miklósi Enikő Kubinyi 《Animal genetics》2013,44(6):717-727
Both dopamine receptor D4 (DRD4) exon 3 and tyrosine hydroxylase (TH) intron 4 repeat polymorphisms have been linked to activity and impulsivity in German Shepherd dogs (GSDs). However, the results in GSDs may not be generalisable to other breeds, as allelic frequencies vary markedly among breeds. We selected the Siberian Husky for further study, because it is highly divergent from most dog breeds, including the GSD. The study sample consisted of 145 racing Siberian Huskies from Europe and North America. We found that this breed possesses seven DRD4 length variants, two to five more variants than found in other breeds. Among them was the longest known allele, previously described only in wolves. Short alleles of the DRD4 and TH repeat polymorphisms were associated with higher levels of activity, impulsivity and inattention. Siberian Huskies possessing at least one short allele of the DRD4 polymorphism displayed greater activity in a behavioural test battery than did those with two long alleles. However, the behavioural test was brief and may not have registered variation in behaviour across time and situations. Owners also completed the Dog‐Attention Deficit Hyperactivity Disorder Rating Scale (Dog‐ADHD RS), a more general measure of activity and attention. Siberian Huskies from Europe with two short alleles of the TH polymorphism received higher ratings of inattention on the Dog‐ADHD RS than did those with the long allele. Investigation of the joint effect of DRD4 and TH showed that dogs possessing long alleles at both sites were scored as less active–impulsive than were others. Our results are aligned with previous studies showing that DRD4 and TH polymorphisms are associated with activity–impulsivity related traits in dogs. However, the prevalence of variants of these genes differs across breeds, and the functional role of specific variants is unclear. 相似文献
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Feigl Gábor Horváth Edit Molnár Árpád Oláh Dóra Poór Péter Kolbert Zsuzsanna 《Journal of Plant Growth Regulation》2019,38(4):1481-1488
Journal of Plant Growth Regulation - Selenium (Se) results in primary root shortening and the concomitant induction of lateral roots (LRs) (stress-induced morphogenic response, SIMR). Both ethylene... 相似文献
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Francesca Rocchi Hiroyuki Oya Fabien Balezeau Alexander J. Billig Zsuzsanna Kocsis Rick L. Jenison Kirill V. Nourski Christopher K. Kovach Mitchell Steinschneider Yukiko Kikuchi Ariane E. Rhone Brian J. Dlouhy Hiroto Kawasaki Ralph Adolphs Jeremy D.W. Greenlee Timothy D. Griffiths Matthew A. Howard Christopher I. Petkov 《Neuron》2021,109(5):852-868.e8
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