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991.
Yu-Lei Liu Yang Zhao Zhong-Min Dai Han-Min Chen Wei-Jun Yang 《The Journal of biological chemistry》2009,284(25):16931-16938
Artemia has attracted much attention for its ability to produce encysted embryos wrapped in a protective shell when subject to extremely harsh environmental conditions. However, what the cyst shell is synthesized from and how the formative process is performed remains, as yet, largely unknown. Over 20 oviparous specifically expressed genes were identified through screening the subtracted cDNA library enriched between oviparous and ovoviviparous Artemia ovisacs. Among them, a shell gland-specifically expressed gene (SGEG) has been found to be involved in the cyst shell formation. Lacking SGEG protein (by RNA interference) caused the cyst shell to become translucent and the chorion layer of the shell to become less compact and pultaceous and to show a marked decrease of iron composition within the shell. The RNA interference induced defective diapause cysts with a totally compromised resistibility to UV irradiation, extremely large temperature differences, osmotic pressure, dryness, and organic solvent stresses. In contrast, the natural cyst would provide adequate protection from all such factors. SGEG contains a 345-bp open reading frame, and its consequentially translated peptide consists of a 33-amino acid residue putative signal peptide and an 81-amino acid residue mature peptide. The results of Northern blotting and in situ hybridization indicate that the gene is specifically expressed in the cells of shell glands during the period of diapause cyst formation of oviparous Artemia. This investigation adds strong insight into the mechanism of cyst shell formation of Artemia and may be applicable to other areas of research in extremophile biology.Salt lakes on plateaus, are widely known as “seas of death,” because they represent one of the most hostile environments on the earth in terms of extreme salinity, high pH, anoxia, large temperature differences, and intermittent dry conditions. Hardly any animal can survive such extremes. However, one notable exception lies in the shape of a small crustacean, Artemia.Artemia, also called the brine shrimp, is an ancient species that first appeared ∼400 million years ago (1). To cope with harsh and complex habitats such as salt lakes, Artemia are able, when the circumstances become adverse, to release their offspring into a dormant, encysted state, rather than simply releasing swimming nauplius, to ensure survival. Such adverse conditions include environments where the Artemia may experience high salinity, low oxygen levels, short days, or conditions of extreme temperature variation (2, 3). These dormant cysts will keep diapause until the state is terminated by activation (triggered by factors such as desiccation, dehydration, cold or chemical treatment), at which point they resume development when appropriate and stable environmental conditions have arisen (4–7).The diapause cysts, with their greatly reduced metabolic activity, contain embryos existing as late gastrulae and are composed of ∼4000 cells that are arrested at the G2/M phase with a complete turning off of RNA and protein synthesis (8, 9). Previous studies indicate that the resistance and resumption ability of Artemia cysts have several causes. In addition to the arrested cell cycle, it has been noted that large amounts of two molecular chaperone proteins, namely p26 and artemin, are synthesized (10–12), and a high concentration of trehalose is also accumulated (13–15). Moreover, a complicated enzyme system is also involved in the diapause and resumption mechanism, including AMP-activated protein kinase (16) and p90 ribosomal S6 kinase regulatory pathway (17–19).In addition to falling into diapause, Artemia themselves secrete a rigid noncellular shell to cope with the extreme environmental stresses before they release the diapause cysts. The complex noncellular cyst shell consists of two main regions; the outer region, secreted by the shell gland, is of hypochlorite-soluble chorion, whereas the hypochlorite-resistant inner region is formed by blastoderm cells and comprises the embryonic cuticle (5, 20, 21). The shell glands, which are composed of clusters of secretory cells, are situated at the ovisac and open into the uterus. There are many dark brown secretory granules, which probably contain chorion material and pigments such as hematin formed in the cells of the shell glands at the point where the oocytes emerge in the ovaries during the reproductive period. These are secreted out at the second day after the oocytes enter the uterus. Therefore the shell glands vary from dark brown to white, even to colorless, as reproductive cycles differ (22, 23).Microphotographs shot by Sugumar and Munuswamy (24) reveal that both the chorion and the embryonic cuticle have an exquisite structure (21). Chorion consists of two distinct layers. First, a compact outer covering is over the cyst with many radially aligned aeropyles penetrating through. This is known as the cortical layer. Second, in a cavernous region below the cortical layer is the alveolar layer, which may act as a float for the newly laid cysts. A thin supra cortical layer, probably consisting of cuticulin, covers the outer surface of the cortical layer. The embryonic cuticle, which is impermeable to nonvolatile solutes, is otherwise composed of a broad multilamellar region as a fibrous layer sandwiched between the outer and inner cuticular membranes and constructed as a tripartite structure. This forms an area of relative independence from the external environment and serves to maintain the homeostasis of inorganic ions (2). The molecular formulation of the cyst shell is complex, and details remain unclear, although it is known that the cyst shell does contain chitin, lipoprotein, hematin, and some metal elements (25–27).Besides preventing mechanical damage (28), the cyst shell also plays an important role in protecting the embryo within from other lethal environmental stresses. Previous experimental data have confirmed the protective capabilities of the cyst shell. Tanguay et al. (29) indicated that the hatching rate of intact cysts is significantly higher than the decapsulated ones after ultraviolet irradiation treatment. Hematin, the hemopigment of the cyst shell, is also demonstrated to have a light-screening function (27). Clegg (30) indicated that the cyst shell plays a critical role in desiccation tolerance, because the rate of dehydration of decapsulated cysts is much higher than intact ones in the dehydration study, and rapid water loss significantly reduces the hatching level of dehydrated cysts. Liu et al. (31) also found that intact cysts have better thermotolerance than decapsulated ones in both dry and water heating studies.In our experiments, through the in vivo gene knockdown by RNA interference, a shell gland-specifically expressed gene (SGEG) has been found to be involved in the cyst shell formation. The formed cyst shell has been demonstrated to play an important role in resistance to UV irradiation, large temperature differences, osmotic pressure, dryness, and organic solution stresses. 相似文献
992.
993.
994.
Bingnan Gu Peng Sun Yuanyang Yuan Ricardo C. Moraes Aihua Li Andy Teng Anshu Agrawal Catherine Rhéaume Virginia Bilanchone Jacqueline M. Veltmaat Ken-Ichi Takemaru Sarah Millar Eva Y.-H.P. Lee Michael T. Lewis Boan Li Xing Dai 《The Journal of cell biology》2009,185(5):811-826
Recent studies have unequivocally identified multipotent stem/progenitor cells in mammary glands, offering a tractable model system to unravel genetic and epigenetic regulation of epithelial stem/progenitor cell development and homeostasis. In this study, we show that Pygo2, a member of an evolutionarily conserved family of plant homeo domain–containing proteins, is expressed in embryonic and postnatal mammary progenitor cells. Pygo2 deficiency, which is achieved by complete or epithelia-specific gene ablation in mice, results in defective mammary morphogenesis and regeneration accompanied by severely compromised expansive self-renewal of epithelial progenitor cells. Pygo2 converges with Wnt/β-catenin signaling on progenitor cell regulation and cell cycle gene expression, and loss of epithelial Pygo2 completely rescues β-catenin–induced mammary outgrowth. We further describe a novel molecular function of Pygo2 that is required for mammary progenitor cell expansion, which is to facilitate K4 trimethylation of histone H3, both globally and at Wnt/β-catenin target loci, via direct binding to K4-methyl histone H3 and recruiting histone H3 K4 methyltransferase complexes. 相似文献
995.
Trichaptum imbricatum, T. perenne and T. podocarpi are three new species from China. They are described, illustrated and compared in this paper with similar species. Trichaptum imbricatum is unique in its imbricate basidiocarps, white to cream hymenophores, small and regular pores, and scattered and thin-walled
cystidia. Trichaptum perenne differs from other species in the genus in its perennial and pileate habit, its large pores and entire dissepiments, and
oblong, ellipsoid basidiospores. Trichaptum podocarpi is distinguished in having totally resupinate basidiocarps, distinctly long cystidia, and in its habitat on Podocarpus. A key to species of Trichaptum occurring in China is provided; statistical variations of spore dimensions for each species are included in the key. 相似文献
996.
997.
Guangyong Zheng ;Hong Li ;Chuan Wang ;Quanhu Sheng ;Haiwei Fan ;Shaoyou Yang ;Boshu Liu ;Jianliang Dai ;Rong Zeng ;Lu Xie 《Acta biochimica et biophysica Sinica》2009,(4):273-279
With the development of functional genomics research, large-scale proteomics studies are now widespread, presenting significant challenges for data storage, exchange, and analysis. Here we present the Integrated Proteomics Exploring Database (IPED) as a platform for managing proteomics experimental data (both process and result data). IPED is based on the schema of the Proteome Experimental Data Repository (PEDRo), and complies with the General Proteomics Standard (GPS) drafted by the Proteomics Standards Committee of the Human Proteome Organization. In our work, we developed three components for the IPED platform: the IPED client editor, IPED server software, and IPED web interface. The client editor collects experimental data and generates an extensible markup language (XML) data file compliant with PEDRo and GPS; the server software parses the XML data file and loads information into a core database; and the web interface displays experimental results, to provide a convenient graphic representation of data. Given software convenience and data abundance, IPED is a powerful platform for data exchange and presents an important resource for the proteomics community. In its current release, IPED is available at http://www. biosino.org/iped2. 相似文献
998.
Platelets are the primary players in both thrombosis and hemostasis. Cyclic AMP (cAMP) and cAMP-dependent protein kinase (PKA)
are important signaling molecules in the regulation of platelet function, such as adhesion, aggregation, and secretion. Elevation
of intracellular cAMP, which induces the activation of PKA, results in the inhibition of platelet function. Thus, tight control
of the intracellular cAMP/PKA signaling pathway has great implications for platelet-dependent hemostasis and effective cardiovascular
therapy. In this review, we summarize the PKA substrates and their contributions to platelet function, especially the advancing
understanding of the cAMP/PKA-dependent signaling pathway in platelet physiology. In addition, we suggest the possibility
that cAMP/PKA is involved in the platelet procoagulant process and receptor ectodomain shedding. 相似文献
999.
Root and shoot traits responses to phosphorus deficiency and QTL analysis at seedling stage using introgression lines of rice 总被引:1,自引:0,他引:1
Phosphorous (P) deficiency is a major restraint factor for crop production and plants have developed several mechanisms to adapt to low P stress. In this study, a set of 271 introgression lines (ILs) were used to characterize the responses of seedlings to low P availability and to identify QTLs for root traits, biomass, and plant height under P-deficiency and P-sufficiency conditions. Plant height, total dry weight, shoot dry weight, and root number were inhibited under P-deficiency, whereas maximum root length (MRL) and root-shoot ratio (RS) were induced by P-deficiency stress. Relative MRL (RMRL, the ratio of MRL under P-deficiency to MRL under P-sufficiency con- dition) and relative RS (RRS) were used to evaluate P-deficiency tolerance at the seedling stage. A total of 24 additive QTLs and 29 pairs of epistatic QTLs were detected, but only qRN4 was detected in both conditions. This suggested that different mechanisms may exist in both P supply levels. QTLs for adaptive traits (RMRL, RRS, RRV, and RRDW) and qRN4 consistently expressed to increase trait stability may contribute to P-deficiency tolerance. Twelve intervals were cluster regions of QTLs for P-deficiency tolerance, and one QTL (qRRSS) showed pleiotropic effects on P-deficiency tolerance and drought tolerance. These interesting QTLs can be used in marker-assisted breeding through the target ILs. 相似文献
1000.