横山水库浮游动物群落结构特征及水质评价

为了解横山水库浮游动物群落结构特征及水质状况,于2012年5月(春)、7月(夏)、10月(秋)及2013年1月(冬)对该水库的浮游动物及主要水质理化指标进行了调查分析,利用生物多样性指数法和综合营养状态指数法评价其水质状况。结果共鉴定浮游动物100种(原生动物47种、轮虫36种、枝角类12种、桡足类5种)。春夏秋季种类组成主要以轮虫和原生动物为主,枝角类与桡足类种类较少;冬季各种类均很少。优势种为王氏似铃壳虫(Tintinnopsis wangi)、淡水简筒虫(Tintinnidium fluviatile)、球形沙壳虫(Difflugia globulosa)、湖生累枝虫(Epistylis lacustris)、裂痕龟纹轮虫(Anuraeopsis fissa)、针簇多肢轮虫(Polyarthra trigla)、裂足臂尾轮虫(Brachionus diversicornis)和等刺异尾轮虫(Trichocerca similis),季节更替明显。浮游动物年均丰度为1460 ind./L,变化范围17—3164 ind./L;年均生物量为1.252 mg/L,变化范围0.042—2.732 mg/L;香农多样性指数(H')、均匀度指数(J)及丰富度指数(D)年均值分别为2.06、0.68及1.30;全年综合营养状态指数值范围33.36—44.15。CCA分析表明,温度及pH等环境因子与浮游动物群落结构具有明显相关性。水质总体评价显示,横山水库水质处中污染状态、水体处中营养水平,基本符合国家Ⅱ类水的水质标准,但作为饮用水水源地,其水质还需进一步加强管理与调控。     

基于温度-伤害度关系分析酿酒葡萄根系及芽抗寒性

采用差热分析系统(DTA)对8个主栽酿酒葡萄品种的芽和根系进行低温放热分析(LTE),建立各品种芽、根系韧皮部及木质部的温度-伤害度(LT-I)回归方程,评估不同品种的根系及芽抗寒性.结果表明： 8个品种的根系韧皮部伤害度50%的温度从高到低为马瑟兰>品丽珠>赤霞珠>小芒森>霞多丽>蛇龙珠>贵人香>熊岳白;不同品种木质部伤害度50%的温度从高到低为马瑟兰>霞多丽>赤霞珠>小芒森>品丽珠>蛇龙珠>贵人香>熊岳白;芽伤害度50%的温度从高到低为赤霞珠>小芒森>蛇龙珠>品丽珠>霞多丽>贵人香>马瑟兰>熊岳白.利用模糊隶属函数值法综合评价根系及芽的抗寒性,马瑟兰根系的抗寒性最差,熊岳白根系的抗寒性最好;赤霞珠、品丽珠、小芒森和蛇龙珠芽的抗寒性最差,贵人香和熊岳白芽的抗寒性最好.     

退耕还湖后湿地土壤对磷的吸附解吸特性

以安徽菜子湖区不同退耕年限(3、5、9和11年)湿地为研究对象,以相邻油菜地和原始湿地为对照(共6个样地),研究退耕还湖后湿地土壤对磷的吸附解吸特性变化.结果表明: 退耕湿地土壤对磷的等温吸附曲线与Langmuir和Freundlich吸附方程拟合程度均达极显著水平(P<0.01);6个样地土壤对磷的最大吸附量(X_m)、吸附常数(K)和最大缓冲容量(MBC)范围分别为478～1074 mg·kg^-1、0.14～0.61和68.6～661.5 mg·kg^-1,且退耕湿地的这些参数均随着退耕年限的延长而升高,但未达到原始湿地水平;6个样地土壤磷的平均解吸率为6.2%～14.6%,且退耕湿地土壤磷解吸率随着退耕年限的延长呈先增高后降低的趋势,但均显著高于原始湿地.表明随着退耕年限的增加,湿地土壤对外源磷的固持能力不断增强,而土壤有机质和粘粒含量是影响湿地土壤对外源磷固持能力的重要因子.     

A simultaneous extraction method for metabolome and lipidome and its application in cry1Ac and sck-transgenic rice leaf treated with insecticide based on LC–MS analysis

Abiotic stress caused by insecticide treatment is an interesting and challenging topic in plant research. Here a simultaneous extraction method with methyl tert-butyl ether for metabolomics and lipidomics analysis by using rapid resolution liquid chromatography quadrupole time-of-flight mass spectrometry was developed and validated. The extraction efficiency of lipidome and metabolome based on the current developed method showed an obvious improvement compared with literatures. About 300 metabolites were identified in rice leaf. Method validation was performed and analytical properties including the linearity (R², 0.991–1.000), repeatability (over 87 % peaks with CV < 20 % accounting for over 92 % of total response) and recovery (74.4–119.6 %) were satisfactory. The method was then applied to investigate time-course changes caused by insecticide treatment in transgenic rice with cry1Ac and sck genes and its wild counterpart. Antioxidants including ferulic acid and sinapic acid were down-regulated at 24 h after insecticide treatment. Signaling metabolites including salicylic acid and nicotinamide adenine dinucleotide were significantly up-regulated at 12–24 h after treatment in transgenic rice. More flavonoids were significantly changed in transgenic plants. Results indicated that gene transformation affected the metabolic response of rice to insecticide stress.     

Regulation of cyp26a1 on Th17 cells in mouse peri‐implantation

Cytochrome P450 26A1 (cyp26a1) is expressed in the mouse uterus during peri‐implantation. The repression of this protein is closely associated with a reduction in implantation sites, suggesting a specific role for cyp26a1 in pregnancy and prompting questions concerning how a metabolic enzyme can generate this distinct outcome. To explore the effective downstream targets of cyp26a1 and confirm if its role in peri‐implantation depends on its metabolic substrate RA (retinoic acid), we characterized the changes in the peripheral blood, spleen and uterine implantation sites using the cyp26a1 gene vaccine constructed before. Flow cytometry results showed a significant increase in CD4⁺RORγt⁺ Th17 cells in both the peripheral blood and spleen in the experimental group. The expression of RORγt and IL‐17 presented the Th17 cells reduction in uterus followed by the suppression of cyp26a1 expression. For greater certainty, cyp26a1 antibody blocking model and RNA interference model were constructed to determine the precise target immune cell group. High performance liquid chromatography results showed a significant increase in uterine at‐RA followed by the immunization of cyp26a1 gene vaccine. Both the ascertain by measuring RARα protein levels in peri‐implantation uterus after gene vaccine immunization and researches using the specific agonist and antagonist against RARα suggested that RARα may be the main RA receptor for signal transduction. These results provided more evidence for the signal messenger role of RA in cyp26a1 regulation from the other side. Here, we showed that the cyp26a1‐regulated Th17 cells are dependent on at‐RA signalling, which is delivered through RARα in mouse peri‐implantation.     

不同光质对小球藻光自养培养积累油脂的影响

研究了5种光质对小球藻(Chlorella vulgaris)M209256生长和产油的影响。结果表明:蓝光为小球藻的最适生长和产油光质;与其他光质相比,蓝光培养的小球藻生物量和油脂含量均较高,为2.40×107个/mL和28%;红光培养的小球藻生长最慢且油脂含量最低,为1.32×107个/mL和15.13%,表现出明显的"红降"现象。在GCMS分析的基础上,对油脂甲酯化后的十六烷值进行评估,结果发现:蓝光的十六烷值最高;5种光质培养的小球藻所产油脂,甲酯十六烷值均在47以上。因此,小球藻油脂所制备的生物柴油具有较好的燃烧性能。     

22例糖尿病视神经病变综合治疗的疗效观察

目的：总结诊断及治疗糖尿病性视神经病变（diabeticopticneuropathy,DON）的临床经验,为本病的治疗和预防提供依据。方法：回顾性研究22例糖尿病视神经痛变的发病特点,在接受治疗的患者中严格控制血糖,应用复方樟柳碱注射液病侧颞浅动脉旁皮下注射,口服或静脉滴注活血化瘀药物,并口服维生素B1、维生素B2、肌苷片等营养视神经的药物,同时给予全身检查,包括对高血压、糖尿病等全身疾病的治疗,观察经综合治疗前后的视力、眼底、视野改变及眼底荧光血管造影（fundusfluoresceinall-giography,FFA）的特点等。结果：接受治疗的患者共有22例（29只眼）,治愈10例（12只眼）;好转7例（10只眼）,总有效率为79．3％。结论：糖尿病性视神经病变的及时正确诊断、系统的综合治疗,可有效提高视力,扩大视野。     

Acetylation control of metabolic enzymes in cancer： an updated version

Metabolic reprogramming is one of the critical features in cancer. Tumor cells preferentially utilize glycolysis instead of oxidative phosphorylation in the presence of oxygen, namely ＇Warburg Effect＇. Recent studies have provided new insights into the Warburg effect, elucidating metabol- ic-dependent and independent mechanisms of metabolic enzymes regulated by post-translational modifications and providing further evidence for the critical role of these tricks in cancer metabolism and tumorigenesis. Of particu- lar interest, we summarized the latest advances in both the metabolic and the non-metabolic functions of metabolic enzymes via the acetylation regulation in the Warburg effect. In addition, their potential roles in cancer metabol- ism therapy will also be briefly discussed.     

Down-regulation of GPR137 expression inhibits proliferation of colon cancer cells

G protein-coupled receptors （GPRs） are highly related to oncogenesis and cancer metastasis. G protein-coupled re- ceptor 137 （GPR137） was initially reported as a novel orphan GPR about 10 years ago. Some orphan GPRs have been implicated in human cancers. The aim of this study is to investigate the role of GPR137 in human colon cancer. Expression levels of GRP137 were analyzed in different colon cancer cell lines by quantitative polymerase chain re- action and western blot analysis. Lentivirus-mediated short hairpin RNA was specifically designed to knock down GPR137 expression in colon cancer cells. Cell viability was measured by methylthiazoletetrazolium and colony forma- tion assays. In addition, cell cycle characteristic was investi- gated by flow cytometry. GRP137 expression was observed in aH seven colon cancer cell lines at different levels. The mRNA and protein levels of GPR137 were down-regulated in both HCTll6 and RKO cells after lentivirus infection. Lentivirus-mediated silencing of GPR137 reduced the proliferation rate and colonies numbers. Knockdown of GPR137 in both cell lines led to cell cycle arrest in the G0/G1 phase. These results indicated that GPR137 plays an important role in colon cancer cell proliferation. A better understanding of GPR137＇s effects on signal transduction pathways in colon cancer cells may provide insights into the novel gene therapy of colon cancer.     

糖尿病合并肺结核患者诱导耐药性危险因素的回归分析

目的:研究2型糖尿病(Type 2 Diabetes,T2DM)合并肺结核(Tuberculosis,TB)患者诱导耐药性危险因素的回归分析。方法:从2012年3月到2013年3月,于我院共计有124例患者被确诊为肺结核,将其作为研究对象。根据患者是否合并有2型糖尿病,将其分成观察组(49例)及对照组(75例)。对全部患者进行耐药性实验,分别经单因素分析及Logistic回归性分析寻找诱导耐药性的危险因素。结果:观察组在治疗过程中断、有吸烟习惯、依从性差、病程≥1年、HbAlc值≥6.5%等方面所占比例显著高于对照组,差异均有统计学意义(均P0.05)。由多因素分析可知,治疗过程中断、有吸烟习惯、依从性差、病程≥1年、HbAlc值≥6.5%等均为糖尿病合并肺结核患者的危险因素。结论:T2DM合并TB患者诱导耐药性的危险因素较多,临床应重点关注,并采取相应措施,从而为临床治疗提供更为有利的条件。