全文获取类型
收费全文 | 3972篇 |
免费 | 274篇 |
国内免费 | 267篇 |
专业分类
4513篇 |
出版年
2024年 | 10篇 |
2023年 | 62篇 |
2022年 | 127篇 |
2021年 | 220篇 |
2020年 | 167篇 |
2019年 | 160篇 |
2018年 | 170篇 |
2017年 | 137篇 |
2016年 | 188篇 |
2015年 | 253篇 |
2014年 | 314篇 |
2013年 | 335篇 |
2012年 | 345篇 |
2011年 | 303篇 |
2010年 | 213篇 |
2009年 | 187篇 |
2008年 | 207篇 |
2007年 | 164篇 |
2006年 | 145篇 |
2005年 | 136篇 |
2004年 | 96篇 |
2003年 | 81篇 |
2002年 | 71篇 |
2001年 | 55篇 |
2000年 | 55篇 |
1999年 | 59篇 |
1998年 | 40篇 |
1997年 | 22篇 |
1996年 | 31篇 |
1995年 | 26篇 |
1994年 | 19篇 |
1993年 | 16篇 |
1992年 | 16篇 |
1991年 | 13篇 |
1990年 | 14篇 |
1989年 | 11篇 |
1988年 | 9篇 |
1987年 | 10篇 |
1986年 | 9篇 |
1985年 | 8篇 |
1984年 | 1篇 |
1983年 | 2篇 |
1981年 | 2篇 |
1979年 | 4篇 |
排序方式: 共有4513条查询结果,搜索用时 10 毫秒
81.
Xiaobo Hu Tingting Duan Zhuan Wu Yuqing Xiong Zhaohui Cao 《Acta biochimica et biophysica Sinica》2021,(7):958-960
In the past few decades,obesity is in an increasingly prevalent metabolic disease worldwide,posing the greatest threat to human health.Understanding the pathway... 相似文献
82.
Xiaobo Hu Tingting Duan Yuqing Xiong Zhijie He Wenjie Wei Zhaohui Cao 《Acta biochimica et biophysica Sinica》2021,(8):1109-1111
Cell-cell communication plays a critical role in cell proliferation,dif-ferentiation,morphogenesis,functiogenesis,and tissue homoeostasis in multicellular organ... 相似文献
83.
84.
85.
Yi‐Zhe Zhang Juncheng Lin Zhizhong Ren Chun‐Xiang Chen Daisuke Miki Si‐Si Xie Jian Zhang Ya‐Nan Chang Jing Jiang Jun Yan Qingshun Q. Li Jian‐Kang Zhu Cheng‐Guo Duan 《植物学报(英文版)》2021,63(4):707-722
Heterochromatin is widespread in eukaryotic genomes and has diverse impacts depending on its genomic context. Previous studies have shown that a protein complex, the ASI1‐AIPP1‐EDM2 (AAE) complex, participates in polyadenylation regulation of several intronic heterochromatin‐containing genes. However, the genome‐wide functions of AAE are still unknown. Here, we show that the ASI1 and EDM2 mostly target the common genomic regions on a genome‐wide level and preferentially interacts with genetic heterochromatin. Polyadenylation (poly(A) sequencing reveals that AAE complex has a substantial influence on poly(A) site usage of heterochromatin‐containing genes, including not only intronic heterochromatin‐containing genes but also the genes showing overlap with heterochromatin. Intriguingly, AAE is also involved in the alternative splicing regulation of a number of heterochromatin‐overlapping genes, such as the disease resistance gene RPP4. We provided evidence that genic heterochromatin is indispensable for the recruitment of AAE in polyadenylation and splicing regulation. In addition to conferring RNA processing regulation at genic heterochromatin‐containing genes, AAE also targets some transposable elements (TEs) outside of genes (including TEs sandwiched by genes and island TEs) for epigenetic silencing. Our results reveal new functions of AAE in RNA processing and epigenetic silencing, and thus represent important advances in epigenetic regulation. 相似文献
86.
Ziqiang Ding Hongwei Duan Wenbo Ge Jianshu Lv Jianlin Zeng Wenjuan Wang Tian Niu Junjie Hu Yong Zhang Xingxu Zhao 《Animal Reproduction》2022,19(2)
Progesterone (P4) can participate in the development of female mammalian antral follicles through nuclear receptor (PGR). In this experiment, the differences of P4 synthesis and PGR expression in different developmental stages of sheep antral follicles (large > 5mm, medium 2-5mm, small < 2mm) were detected by enzyme-linked immunosorbent assay, immunohistochemistry, qRT-PCR and Western blotting. Secondly, sheep follicular granulosa cells were cultured in vitro. The effects of different concentrations of FSH and LH on P4 synthesis and PGR expression were studied. The results showed that acute steroid regulatory protein (StAR), cholesterol side chain lyase (P450scc) and 3β Hydroxysteroid dehydrogenase (3β-HSD) and PGR were expressed in antral follicles, and with the development of antral follicles in sheep, StAR, P450scc and the expression of 3β-HSD and PGR increased significantly. In vitro experiments showed that FSH and LH alone or together treatment could regulate P4 secretion and PGR expression in sheep follicular granulosa cells to varying degrees, hint P4 and PGR by FSH and LH, and LH was the main factor. Our results supplement the effects of FSH and LH on the regulation of P4 synthesis during follicular development, which provides new data for further study of steroid synthesis and function in follicular development. 相似文献
87.
88.
Bingqing Xia Xurui Shen Yang He Xiaoyan Pan Feng-Liang Liu Yi Wang Feipu Yang Sui Fang Yan Wu Zilei Duan Xiaoli Zuo Zhuqing Xie Xiangrui Jiang Ling Xu Hao Chi Shuangqu Li Qian Meng Hu Zhou Yubo Zhou Xi Cheng Xiaoming Xin Lin Jin Hai-Lin Zhang Dan-Dan Yu Ming-Hua Li Xiao-Li Feng Jiekai Chen Hualiang Jiang Gengfu Xiao Yong-Tang Zheng Lei-Ke Zhang Jingshan Shen Jia Li Zhaobing Gao 《Cell research》2021,31(8):847-860
Cytokine storm and multi-organ failure are the main causes of SARS-CoV-2-related death. However, the origin of excessive damages caused by SARS-CoV-2 remains largely unknown. Here we show that the SARS-CoV-2 envelope (2-E) protein alone is able to cause acute respiratory distress syndrome (ARDS)-like damages in vitro and in vivo. 2-E proteins were found to form a type of pH-sensitive cation channels in bilayer lipid membranes. As observed in SARS-CoV-2-infected cells, heterologous expression of 2-E channels induced rapid cell death in various susceptible cell types and robust secretion of cytokines and chemokines in macrophages. Intravenous administration of purified 2-E protein into mice caused ARDS-like pathological damages in lung and spleen. A dominant negative mutation lowering 2-E channel activity attenuated cell death and SARS-CoV-2 production. Newly identified channel inhibitors exhibited potent anti-SARS-CoV-2 activity and excellent cell protective activity in vitro and these activities were positively correlated with inhibition of 2-E channel. Importantly, prophylactic and therapeutic administration of the channel inhibitor effectively reduced both the viral load and secretion of inflammation cytokines in lungs of SARS-CoV-2-infected transgenic mice expressing human angiotensin-converting enzyme 2 (hACE-2). Our study supports that 2-E is a promising drug target against SARS-CoV-2.Subject terms: Cell death, Molecular biology 相似文献
89.
Tingting Duan Cifei Tang Zhuan Wu Zhaohui Cao Xiaobo Hu 《Acta biochimica et biophysica Sinica》2021,(1):128-130
Type 2 diabetes(T2D)is a chronic metabolic disease characterized by insulin resistance and hyperglycemia,which is ultimately linked to the loss of pancreaticβ-cells and their function[1].Understanding the pathological mechanisms ofβ-cell dysfunction in T2D may lead to development of new therapeutic approaches.Recently,compelling evidence suggests that members of the nuclear receptor 4A(NR4A)subgroup play a pivotal role inβ-cell loss[2].Nor1,also known as NR4A3,belongs to the NR4A subfamily,which also includes Nur77(NR4A1)and Nurr1(NR4A2),and is defined as a true orphan nuclear receptor with an unknown endogenous ligand or ligand independent[3].As a regulator of gene expression located in the nucleus,Nor1 exhibits tissue-specific expression,which selectively controls diverse biological processes,including cell proliferation,apoptosis,differentiation,immune homeostasis,and fuel utilization[4].Thus far,it was reported that Nor1 is involved in numerous pathologies such as cancer,inflammatory diseases,and Parkinson’s disease[4]. 相似文献
90.
Fabrice Roegiers Joshua Kavaler Nicholas Tolwinski Yu-Ting Chou Hong Duan Fernando Bejarano Diana Zitserman Eric C. Lai 《Genetics》2009,182(1):407-410
Forty years ago, a high frequency of lethal giant larvae (lgl) alleles in wild populations of Drosophila melanogaster was reported. This locus has been intensively studied for its roles in epithelial polarity, asymmetric neural divisions, and restriction of tissue proliferation. Here, we identify a high frequency of lgl alleles in the Bloomington second chromosome deficiency kit and the University of California at Los Angeles Bruinfly FRT40A-lethal P collection. These unrecognized aberrations confound the use of these workhorse collections for phenotypic screening or genetic mapping. In addition, we determined that independent alleles of insensitive, reported to affect asymmetric cell divisions during sensory organ development, carry lgl deletions that are responsible for the observed phenotypes. Taken together, these results encourage the routine testing of second chromosome stocks for second-site alleles of lgl. 相似文献