Untersuchungen Über faktoren,die sich bei folgekultivierung der pflanzen geltend machen

V práci byla sledována mo?nost allelopatického ovlivňování následných rostlin p?edplodinami p?i kultivaoi v odstupňovaných ?asových intervalech po sobě v té?e zemině u tě chto kombinací: mák — cukrovka, ho??ice — je?men, konopí — ?ito, cibule — ?epka. Pokusy byly prováděny v kvítiná?ích s kompostovou zeminou, umístěných během pokusu na zahradě a zapu? těných do p?dy. Byl sledován r?st p?edplodin a následných rostlin v po?áte?ních fá zích r?stu. P?ed vysetím následných rostlin byla stanovena u odebraných vzork? zemin intensita respirace, okam?itá vlhkost a obsah fyziologicky p?ístupného dusíku, fosforu a draslíku. Ve v?ech zkou?ených kombinacích byly následné rostliny ovlivněny kultivací p?edplodiny a následným ulo?ením zeminy. Změny r?stu následných rostlin ?áste? ně korelovaly s obsahem fyziologicky p?ístupného dusíku v zemině. Podle jejich charakteru v?ak bylo té? patrno, ?e se na nich podílely i allelopatické faktory. Zna?ně inhibi?ně p?sobil mák na cukrovku, mé ně inhibi?ně p?sobila ho??ice na je?men a cibule na ?epku. ??inek konopí na ?ito byl promě nný s dobou ulo?ení zeminy. Změny v obsahu fyziologicky p?ístupného dusíku, fosforu a draslíku v pokusné zemině neodpovídaly . mno?ství narostlé p?edplodiny, co? bylo podmíněno pou?itou kultiva?ní metodikou. Poměrně rychlé doplňování p?edplodinou vy?erpaných dusi?nan? v pokusné zemině s dobou jejího ulo?ení bylo pravděpodobně podmíněno nitrifikacními procesy. Podle stanovených změn intensity respirace pokusné zeminy se na allelopatickém ovlivnění mohla podílet i pudní mikroflora.     

Analysis of replication region of the cryptic plasmid pAG20 from Acetobacter aceti 3620

The DNA sequence of small cryptic plasmid pAG20 in Acetobacter aceti was determined at 3064 bp with 51.6% GC pairs. The plasmid encoded a 186 amino acid protein which is important for plasmid replication in Gram-negative bacteria except Escherichia coli. Two 21 bp large direct repeat sequence 1 and two 13 bp direct repeat sequence 2 were determined in the regulation region upstream from gene encoded Rep protein. Vector pAG24 with kanamycin gene and two deletion derivatives pAG25 and pAG26 without rep gene from plasmid pAG20 were constructed. Plasmid pAG24 was replicated in a broad host range like E. coli, Acetobacter pasteurianus, A. aceti, Comanomonas spp., Serratia marcescens, and Shigella spp.     

Bacterial DNA detected on pathologically changed heart valves using 16S rRNA gene amplification

Nowadays, dental diseases are one of the most common illnesses in the world. Some of them can lead to translocation of oral bacteria to the bloodstream causing intermittent bacteraemia. Therefore, a potential association between oral infection and cardiovascular diseases has been discussed in recent years as a result of adhesion of oral microbes to the heart valves. The aim of this study was to detect oral bacteria on pathologically changed heart valves not caused by infective endocarditis. In the study, patients with pathologically changed heart valves were involved. Samples of heart valves removed during heart valve replacement surgery were cut into two parts. One aliquot was cultivated aerobically and anaerobically. Bacterial DNA was extracted using Ultra-Deep Microbiome Prep (Molzym GmbH, Bremen, Germany) followed by a 16S rRNA gene PCR amplification using Mastermix 16S Complete kit (Molzym GmbH, Bremen, Germany). Positive PCR products were sequenced and the sequences were analyzed using BLAST database (http://www.ncbi.nlm.nih/BLAST). During the study period, 41 samples were processed. Bacterial DNA of the following bacteria was detected in 21 samples: Cutibacterium acnes (formerly Propionibacterium acnes) (n?=?11; 52.38% of patients with positive bacterial DNA detection), Staphylococcus sp. (n?=?9; 42.86%), Streptococcus sp. (n?=?1; 4.76%), Streptococcus sanguinis (n?=?4; 19.05%), Streptococcus oralis (n?=?1; 4.76%), Carnobacterium sp. (n?=?1; 4.76%), Bacillus sp. (n?=?2; 9.52%), and Bergeyella sp. (n?=?1; 4.76%). In nine samples, multiple bacteria were found. Our results showed significant appearance of bacteria on pathologically changed heart valves in patients with no symptoms of infective endocarditis.     

The <Emphasis Type="Italic">Agropyron cristatum</Emphasis> karyotype,chromosome structure and cross-genome homoeology as revealed by fluorescence in situ hybridization with tandem repeats and wheat single-gene probes

Key message

Fluorescence in situ hybridization with probes for 45 cDNAs and five tandem repeats revealed homoeologous relationships of Agropyron cristatum with wheat. The results will contribute to alien gene introgression in wheat improvement.

Abstract

Crested wheatgrass (Agropyron cristatum L. Gaertn.) is a wild relative of wheat and a promising source of novel genes for wheat improvement. To date, identification of A. cristatum chromosomes has not been possible, and its molecular karyotype has not been available. Furthermore, homoeologous relationship between the genomes of A. cristatum and wheat has not been determined. To develop chromosome-specific landmarks, A. cristatum genomic DNA was sequenced, and new tandem repeats were discovered. Their distribution on mitotic chromosomes was studied by fluorescence in situ hybridization (FISH), which revealed specific patterns for five repeats in addition to 5S and 45S ribosomal DNA and rye subtelomeric repeats pSc119.2 and pSc200. FISH with one tandem repeat together with 45S rDNA enabled identification of all A. cristatum chromosomes. To analyze the structure and cross-species homoeology of A. cristatum chromosomes with wheat, probes for 45 mapped wheat cDNAs covering all seven chromosome groups were localized by FISH. Thirty-four cDNAs hybridized to homoeologous chromosomes of A. cristatum, nine hybridized to homoeologous and non-homoeologous chromosomes, and two hybridized to unique positions on non-homoeologous chromosomes. FISH using single-gene probes revealed that the wheat-A. cristatum collinearity was distorted, and important structural rearrangements were observed for chromosomes 2P, 4P, 5P, 6P and 7P. Chromosomal inversions were found for pericentric region of 4P and whole chromosome arm 6PL. Furthermore, reciprocal translocations between 2PS and 4PL were detected. These results provide new insights into the genome evolution within Triticeae and will facilitate the use of crested wheatgrass in alien gene introgression into wheat.

     

Construction of a simple flat magnetic separator

Summary A procedure for the construction of a cheap and simple flat magnetic separator is described. The separator can be used to remove magnetic carriers and sorbents from up to 500 – 1000 ml of suspension. The properties of this model and commercially available separators are similar.