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61.
Frederico Figueiredo Amancio Tiago Pires Heringer Cristina da Cunha Hueb Barata de Oliveira Liliane Boaventura Fassy Frederico Bruzzi de Carvalho Daniela Pagliari Oliveira Claudio Dornas de Oliveira Fernando Otoni Botoni Fernanda do Carmo Magalh?es José Roberto Lambertucci Mariangela Carneiro 《PloS one》2015,10(6)
The purpose of our study was to describe the clinical profile of dengue-infected patients admitted to Brazilian intensive care units (ICU) and evaluate factors associated with death. A longitudinal, multicenter case series study was conducted with laboratory-confirmed dengue patients admitted to nine Brazilian ICUs situated in Minas Gerais state, southeastern Brazil from January 1, 2008, to December 31, 2013. Demographic, clinical and laboratory data; disease severity scores; and mortality were evaluated. A total of 97 patients were studied. The in-ICU and in-hospital mortality rates were 18.6% and 19.6%, respectively. Patients classified as having severe dengue according to current World Health Organization classifications showed an increased risk of death in a univariate analysis. Nonsurvivors were older, exhibited lower serum albumin concentrations and higher total leukocyte counts and serum creatinine levels. Other risk factors (vomiting, lethargy/restlessness, dyspnea/respiratory distress) were also associated with death in a univariate analysis. Multivariate analysis indicated that in-hospital mortality was significantly associated with Acute Physiology and Chronic Health Evaluation II and the Sequential Organ Failure Assessment score. The ICU and in-hospital mortality observed in this study were higher than values reported in similar studies. An increased frequency of ICU admission due to severe organ dysfunction, higher severity indices and scarcity of ICU beds may partially explain the higher mortality. 相似文献
62.
Fabricio Fernandes Fontana Celso Tadeu Barbosa dos Santos Flavia Maria Esteves Ademir Rocha Geisa Ferreira Fernandes Cristiane Candida do Amaral Marcos Abel Domingues Zoilo Pires De Camargo Mario León Silva-Vergara 《Mycopathologia》2010,169(3):159-165
There is some evidence that dogs can be naturally infected by Paracoccidioides brasiliensis in endemic areas of paracoccidioidomycosis. In order to evaluate canine infection with this fungus, a survey with 149 urban
and 126 rural dogs was carried out using ELISA and intradermal tests with the gp43 antigen of P. brasiliensis in Uberaba, Minas Gerais state of Brazil. Forty-one out of 149 urban dogs were euthanatized and had their lungs, liver and
spleen removed. One slice from each viscera was processed for histopathological examination and the remaining was homogenized and then cultivated on mycobiotic agar
at room temperature and Fava-Netto medium at 35°C and observed for 12 weeks. Of urban dogs, 75 (50.3%) were small adult females,
56 (36%) were strays, while 93 (64%) had been donated to the municipal zoonosis control center. Nine (6.2%) had a positive
intradermal test without statistical differences regarding gender, race, nutritional status or origin. No colonies with microscopic
or morphology appearances resembling P. brasiliensis were isolated, nor granulomatous process or fungal structures were observed from histopathological examination. Eighty (53.6%)
of the urban dogs presented seroreactivity, without statistical differences regarding gender, race, nutritional state, origin,
or positive intradermal test. Of 126 rural dogs, 102 (80.5%) presented antibodies against gp43 antigen, and this was statistically
significant in relation to the reactivity detected in urban dogs (P = 0.0001). Thus, dogs are commonly infected with P. brasiliensis, but they probably present natural resistance to develop paracoccidioidomycosis. 相似文献
63.
Patricia Fagundes Costa Geisa Ferreira Fernandes Priscila Oliveira dos Santos Cristiane Candida Amaral Zoilo Pires Camargo 《Mycopathologia》2010,169(1):37-46
The ecological niche or exact habitat of the fungus Paracoccidioides brasiliensis is not known, and few isolates have been obtained from the environment. In this study, ten isolates were analyzed with respect
to antigenic composition, serology, pathogenicity, and molecular aspects. Gp43 is considered to be the molecular basis for
the serodiagnosis of paracoccidioidomycosis; however, in this study only six of the environmental isolates secreted this molecule
(four in great amounts and two in small amounts). Other molecules were also produced. When exoantigens from these isolates
were tested using immunodiffusion, only four preparations were positive by ID tests. However, when these exoantigens were
tested by ELISA, all of them except one were able to detect anti-P. brasiliensis antibodies. In Western blot assays, these exoantigens showed different reactivities. Isolates that secreted gp43 presented
positive reactions for this molecule, and isolates that did not secrete gp43 gave positive reactions for other minor molecules.
RAPD analysis revealed that there is great genetic variation between these environmental isolates. These isolates were non-pathogenic:
no mortality was observed among the inoculated mice during an 18-month follow-up period. 相似文献
64.
Bárbara Santos Pires da Silva Eloísa Bonfá Júlio César Bertacini de Moraes Carla Gonçalves Schain Saad Ana Cristina de Medeiros Ribeiro Célio Roberto Gonçalves Jozélio Freire de Carvalho 《Biologicals》2010,38(5):567-569
The objective of this study was to evaluate the influence of anti-tumor necrosis factor (anti-TNF) in juvenile idiopathic arthritis (JIA), ankylosing spondylitis (AS) or psoriatic arthritis (PsA). Sixty-two patients were investigated: 7 JIA; 37 AS; and 18 PsA. Caucasian race accounted for 79% and 29% were female. Mean age was 40.4 ± 12.6years. None of the patients had a history of diabetes, and none had used oral hypoglycemic agents or insulin. Treatment was with adalimumab, infliximab and etanercept. Glucose, inflammatory markers and prednisone dose were assessed at baseline, as well as after three and six months of treatment. The mean erythrocyte sedimentation rate was significantly lower at three months and six months than at baseline (13.7 ± 18.0 and 18 ± 22.5 vs. 27.9 ± 23.4 mm; p = 0.001). At baseline, three months and six months, we found the following: mean C-reactive protein levels were comparable (22.1 ± 22.7, 14.5 ± 30.7 and 16.0 ± 23.8 mg/L, respectively; p = 0.26); mean glucose levels remained unchanged (90.8 ± 22.2 mg/dl, 89.5 ± 14.6 mg/dl and 89.8 ± 13.6 mg/dl, respectively; p = 0.91); and mean prednisone doses were low and stable (3.9 ± 4.9 mg/day, 3.7 ± 4.8 mg/day and 2.6 ± 4.0 mg/day, respectively; p = 0.23). During the first six months of treatment, anti-TNF therapy does not seem to influence glucose metabolism in JIA, AS or PsA. 相似文献
65.
Filipe Anibal Carvalho‐Costa Silvia M Dos Santos Marize Quinhones Pires Catarina Macedo Lopes François Noireau Raquel S. Pacheco 《Journal of vector ecology》2010,35(2):295-300
Chagas disease remains a public health concern in Brazil and other Latin American countries, mainly due to the potential domiciliation of native triatomine species. We analyzed the genetic variability of Triatoma pseudomaculata in sylvatic and peridomestic ecotopes throughout three localities in the northeastern state of Bahia, Brazil. We studied polymorphisms generated by random amplified polymorphic DNA (RAPD) and isoenzyme electrophoresis analyses. Based on RAPD analysis, each specimen was assigned to one of three genetic clusters. Although all sylvatic specimens from one locality were grouped into the same cluster, sylvatic and peridomestic specimens from the other two localities were broadly distributed between the remaining two clusters, suggesting that geographic population structuring was not occurring. Furthermore, isoenzyme analysis suggested that distinct populations were in Hardy‐Weinberg equilibrium. Low statistical values for Wright's Fst index also supported the absence of population structuring and suggested the occurrence of panmixia. We conclude that genetic flow occurs between sylvatic and peridomestic T. pseudomaculata populations, probably as a consequence of passive and active dispersion of the insects, associated with deforestation and anthropic transformations. 相似文献
66.
Maria Cristina Sanches Sérvio Pontes Ribeiro Valdnéa Casagrande Dalvi Milton Barbosa da Silva Junior Hildeberto Caldas de Sousa José Pires de Lemos-Filho 《Trees - Structure and Function》2010,24(1):79-88
Cariniana legalis is an emergent tree that reaches the upper canopy in Brazilian Semideciduous Forest. Spatial contrasts in microclimatic conditions
between the upper canopy and understorey in a forest may affect morpho-physiological leaf traits. In order to test the hypothesis
that the upper canopy is more stressful to leaves than a gap environment we compared emergent trees of C. legalis, 28–29 m in height to gap saplings, 6–9 m in height, for the following parameters: leaf area, leaf mass area (LMA the dry
weight:leaf area ratio), leaf thickness, leaf anatomical parameters, stomata conductance, and chlorophyll a fluorescence. Leaves from emergent trees had smaller leaf areas but greater LMA compared to saplings. Leaf thickness, palisade
layer thickness, and stomatal density were higher for emergent trees than for saplings. The opposite pattern was observed
for spongy layer thickness and spongy/palisade ratio. Stomatal conductance was also higher for emergent tree leaves than for
sapling leaves, but the magnitude of depression on stomatal conductance near midday was more pronounced in emergent trees.
The potential quantum yield of photosystem II, as determined by the F
v/F
m ratio was lower for leaves from saplings. The lower values of stomatal conductance, indicating restriction in CO2 diffusion into the mesophyll can be related to higher photoinhibition observed in the saplings. Leaves from emergent trees
and saplings exhibited similar values for apparent electron transport rates and non-photochemical quenching. Our results suggest
that changes in leaf traits could be associated to dry conditions at the upper canopy as well as to the ontogenetic transition
between sapling/emergent tree life stages. 相似文献
67.
Adriano Caliman Aliny F. Pires Francisco A. Esteves Reinaldo L. Bozelli Vinicius F. Farjalla 《Biodiversity and Conservation》2010,19(3):651-664
The sub-discipline of biodiversity and ecosystem functioning (BEF) has emerged as a central topic in contemporary ecological
research. However, to date no study has evaluated the prominence and publication biases in BEF research. Herein we report
the results of a careful quantitative assessment of BEF research published in five core general ecology journals from 1990
to 2007 to determine the position of BEF research within ecology, identify patterns of research effort within BEF research,
and discuss their probable proximal and historical causes. The relative importance of BEF publications increased exponentially
during the period analyzed and was significantly greater than the average growth of ecological literature, affirming the prominence
of BEF as a current paradigm in ecology. However, BEF research exhibited a strong bias toward experimental studies on terrestrial
plant communities, with significantly lower effort devoted to the functional aspects of biodiversity in aquatic systems, multiple
trophic level systems, and animal or microbial communities. Such trends may be explained by a combination of methodological
adequacy and historic epistemological differences in ecological thinking. We suggest that BEF researchers should direct more
effort toward the study of aquatic systems and animal communities, emphasize long-term and trophically complex experiments,
such as those with multi-trophic microbial communities, employ larger-scale field observational studies and increase the use
of integrative and theoretical studies. Many technical and analytical methodologies that are already employed in ecological
research, such as stable isotopes, paleobiology, remote sensing, and model selection criteria, can facilitate these aims.
Overcoming the above-mentioned shortcomings of current BEF research will greatly improve our ability to predict how biodiversity
loss will affect ecosystem processes and services in natural ecosystems. 相似文献
68.
Early evolution of bHLH proteins in plants 总被引:1,自引:0,他引:1
69.
Lívia Santos Lima Karina Peres Gramacho José Luis Pires Didier Clement Uilson Vanderlei Lopes Nicolas Carels Abelmon da Silva Gesteira Fernanda Amato Gaiotto Júlio Cézar de Mattos Cascardo Fabienne Micheli 《Tree Genetics & Genomes》2010,6(5):663-676
In this study, we report results of the detection and analysis of SSR markers derived of cacao–Moniliophthora perniciosa expressed sequence tags (ESTs) in relation to cacao resistance to witches’ broom disease (WBD), and we compare the polymorphism
of those ESTs (EST-simple sequence repeat (SSR)) with classical neutral SSR markers. A total of 3,487 ESTs was used in this
investigation. SSRs were identified in 430 sequences: 277 from the resistant genotype TSH 1188 and 153 from the susceptible
one Catongo, totalizing 505 EST-SSRs with three types of motifs: dinucleotides (72.1%), trinucleotides (27.3%), and tetranucleotides
(0.6%). EST-SSRs were classified into 16 main categories; most of the EST-SSRs belonged to “Unknown function” and “No homology”
categories (45.82%). A high frequency of SSRs was found in the 5’UTR and in the ORF (about 27%) and a low frequency was observed
in the 3’UTR (about 8%). Forty-nine EST-SSR primers were designed and evaluated in 21 cacao accessions, 12 revealed polymorphism,
having 47 alleles in total, with an average of 3.92 alleles per locus. On the other hand, the 11 genomic SSR markers revealed
a total of 47 alleles, with an average of 5.22 alleles per locus. The association of EST-SSR with the genomic SSR enhanced
the analysis of genetic distance among the genotypes. Among the 12 polymorphic EST-SSR markers, two were mapped on the F2 Sca 6 × ICS 1 population reference for WBD resistance. 相似文献
70.
Marreiro DN do Perpetuo Socorro C Martins M de Sousa SS Ibiapina V Torres S Pires LV do Nascimento Nogueira N Lima JM do Monte SJ 《Biological trace element research》2007,120(1-3):42-50
The objective of this study was to assess urinary excretion of zinc and evaluation parameters of metabolic control in type
2 diabetic patients. Thirty-one type 2 diabetic patients, of both genders, with 5.8 ± 5.6 years average time of the disease,
age range 20–60 years, were selected. Evaluation of the nutritional status was performed using anthropometric measurements.
To evaluate food consumption, the 3-day alimentary log method was used, and its analysis was performed using a software. Determination
of urinary zinc was by atomic absorption spectrophotometry. From the obtained results, it was concluded that 51.6% of the
patients were overweight. The mean of found waist circumference was 100.4 and 92.2 cm for men and women, respectively. Blood
glucose and glycated hemoglobin values were higher than reference values, and plasma albumin concentration was adequate. The
median of found urinary zinc excretion was 474.9 μg/24 h, within normal standards (300–600 μg/day). Regarding diet composition,
calorie and protein concentration were above recommendation, while mean zinc concentration was adequate. This data allow the
conclusion that the evaluated patients presented adequate urinary zinc excretion in comparison with reference values. 相似文献