Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine

The growth characteristics and influence of glucose and glutamine on the growth and maintenance of channel catfish ovary (CCO) cells were investigated. Besides glutamine, amino acids threonine, arginine, methionine and serine were found to be essential for CCO cell growth. In the glucose-free medium, glutamine is utilized as energy source and no cell growth limitation was observed. However, the lack of glutamine in culture medium did not stimulate CCO cells to efficient glucose consumption. When both glucose and glutamine were deficient, cell growth was also observed suggesting no rigorous nutritional requirements. Obtained results are useful for further understanding of culture processes using CCO cells.     

The effect of aluminium on the heam biosynthesis (in vitro) in bone marrow cells in rats.

The study has been carried out on male Wistar rats. The aim of the present study was to trace the effect of aluminium chloride and aluminium nitrate at concentration 10 microM and 20 microM on haem biosynthesis in vitro in bone marrow cell culture. The ability of haem biosynthesis in bone marrow cell culture after 48 h of experiments with aluminium chloride and aluminium nitrate significantly decreased in relation with the control value.