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81.
Z. N. Zhuravleva S. S. Hutsyan G. I. Zhuravlev 《Russian Journal of Developmental Biology》2016,47(3):147-153
Neurochemical differentiation of neurons in transplants developing in rat anterior eye chamber was studied. Pieces of the somatosensory neocortex area, isolated from 17-day fetuses of Wistar rats, were used for the transplantation. The general cytological analysis and immunochemical identification of GABAergic neurons in neocortical transplants and in the appropriate brain area of the recipient rats (control) were carried out after 6 months. Cytoarchitectonics typical for neocortex was not revealed in the transplants. Furthermore, a 1.4-fold decrease in numerical density of the entire neuron population was found compared to the control. The proportion of GABAergic nerve cells in the transplanted tissue was reduced even more dramatically— by 13.1 times. The dimensions of all types of neurons, especially GABAergic cells, were greater in the transplants in oculo compared to neocortex in situ. The increase in size occurred mostly due to the cytoplasm. Thus, the nuclei of GABA-positive neurons in the transplants were larger by 1.2 times compared to the control and their perikarya were larger by 1.5 times. The obtained results showed that the conditions in the anterior eye chamber the most dramatically affect the differentiation of GABAergic neurons, and cell hypertrophy, probably, is the functional compensation of the decrease in their number. Considering the literature data on the increased excitability and synchronized neuronal activity in the intraocular transplants, it can be assumed that these transplants can be used as a model for studying the cellular mechanisms of nervous tissue epileptization under disinhibition conditions. 相似文献
82.
The composition of nitrogen-containing organic bases in thevolatile substances of boiled beef has been studied. Thirtycompounds have been identified including primary, secondary,and tertiary aliphatic amines, pyrazines, pyridine, piperidineand their alkyl substituted derivatives. The content of trimethyl-,diethyl-, diisopropylamines, and piperidine, possible precursorsof N-nitrosamines, has been quantitatively determined. 相似文献
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O. V. Tupikina V. S. Melamud T. I. Bogdanova I. A. Tsaplina T. A. Pivovarova A. E. Zhuravleva T. F. Kondrat’eva 《Microbiology》2009,78(5):593-597
Plasmids were discovered for the first time in strains belonging to different species of the genus Sulfobacillus: S. thermosulfidooxidans, S. sibiricus, S. thermotolerans, “S. olympiadicus”, and S. acidophilus. The plasmids were detected in the cells of four out of eight strains grown on a medium with ferrous iron. Adaptation to
elementary sulfur was accompanied by changes in the plasmid profiles in two out of seven strains. Plasmids were detected in
all the studied strains of sulfobacilli after adaptation to the pyrite-arsenopyrite ore concentrate from the Nezhdaninskoe
deposit containing gold, silver, zinc, copper, and lead. No plasmids were found in S. thermotolerans Kr1T after four transfers on a medium containing iron and 0.018 mM Ag+. After adaptation of the same strain to 765 mM Zn2+, only one plasmid was found in the cells, the largest among those detected earlier in this culture adapted to the Nezhdaninskoe
ore concentrate. The strain S. thermotolerans Kr1T, after four transfers on media with either 78 mM Cu2+ or 2 mM Pb2+, did not contain plasmids. The presence of plasmids in the cells of sulfobacilli did not influence their resistance to the
ions of the studied metals. 相似文献
86.
Ivan L. Budyak Beena Krishnan Anna M. Marcelino-Cruz Mylene C. Ferrolino Anastasia Zhuravleva Lila M. Gierasch 《Structure (London, England : 1993)》2013,21(3):476-485
Highlights? The folding pathway of a β-barrel protein protects it from aggregation ? The rate-determining transition state of CRABP1 is polarized and malleable ? Regions constituting the aggregate core of CRABP1 are protected early in folding ? Early barrel closure in iLBPs may offer a general strategy for productive folding 相似文献
87.
It has been found by reversed-phase chromatography that humic acids obtained from vermicomposts of different duration of vermicomposting consist of a hydrophilic and a hydrophobic fractions, the hydrophobic fraction having a substantially lower content of charged, probably carboxylic, groups. A change in the sign of the temperature dependence of the heat capacity of diluted aqueous solutions of humic acids at approximately 58 degrees C has been found by differential scanning microcalorimetry, which indicates an increase in the hydration of hydrophobic groups. A jumpwise increase in heat capacity in the temperature range from 86 to 90 degrees C was also found, which is due likely to the hydration of hydrophobic groups in the interior of "micelles", due to the "devitrification" of the hydrophobic nucleus of micelle-like structures. It was shown that increasing the duration of vermicomposting leads to an increase in the relative content of the hydrophobic fraction of humic acids and in the cooperativity of the thermodynamic transition, which manifests itself in a jump of heat capacity, which probably results from the increase in the "micelle" size. 相似文献
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Catechol 2, 3-dioxygenase is present in several types of bacteria and undergoes degradation of environmental pollutants through
an important key biochemical pathways. Specifically, this enzyme cleaves aromatic rings of several environmental pollutants such
as toluene, xylene, naphthalene and biphenyl derivatives. Hence, the importance of Catechol 2, 3-dioxygenase and its role in the
degradation of environmental pollutants made us to predict the three-dimensional structure of Catechol 2, 3-dioxygenase from
Burkholderia cepacia. The 10ns molecular dynamics simulation was carried out to check the stability of the modeled Catechol 2, 3-
dioxygenase. The results show that the model was energetically stable, and it attains their equilibrium within 2000 ps of production
MD run. The docking of various petroleum hydrocarbons into the Catechol 2,3-dioxygenase reveals that the benzene, O-xylene,
Toluene, Fluorene, Naphthalene, Carbazol, Pyrene, Dibenzothiophene, Anthracene, Phenanthrene, Biphenyl makes strong
hydrogen bond and Van der waals interaction with the active site residues of H150, L152, W198, H206, H220, H252, I254, T255,
Y261, E271, L276 and F309. Free energy of binding and estimated inhibition constant of these compounds demonstrates that they
are energetically stable in their binding cavity. Chrysene shows positive energy of binding in the active site atom of Fe. Except
Pyrene all the substrates made close contact with Fe atom by the distance ranges from 1.67 to 2.43 Å. In addition to that, the above
mentioned substrate except pyrene all other made π-π stacking interaction with H252 by the distance ranges from 3.40 to 3.90 Å.
All these docking results reveal that, except Chrysene all other substrate has good free energy of binding to hold enough in the
active site and makes strong VdW interaction with Catechol-2,3-dioxygenase. These results suggest that, the enzyme is capable of
catalyzing the above-mentioned substrate. 相似文献
90.