辽西半干旱地区深松中耕对作物产量的影响及其作用机理研究

对辽宁西部半干旱地区深松中耕对作物产量影响及作用机理的研究表明,深松打破了犁底层,减小了土壤容重及穿透阻力,增加了表层及亚表层土壤的孔隙度．深松土壤水分人渗可达８０ｃｍ,大豆根系深度增加３．６～４．０ｃｍ,大豆产量和水分利用效率分别增加２７．７～３４．５％和１６．１％,在干旱年份玉米增产７．９％．     

Combined DNA vaccines formulated in DDA enhance protective immunity against tuberculosis

This study evaluated the adjuvant Dimethyldioctyldecyl Ammonium Bromide (DDA) effect on the protective immunity induced by a combination of plasmids containing genes encoding antigens Ag85B, MPT-83, and ESAT-6 from Mycobacterium tuberculosis. The combined DNA vaccines in DDA resulted in significant increases in both specific IgG and splenic T-cell-derived Th1-type cytokine gamma interferon (IFN-gamma) production in response to the three purified antigens when compared to that of combined DNA vaccines in saline. Vaccines in DDA increased the protective efficacy of mice challenged with M. tuberculosis H37Rv as measured by reduced relative CFU counts in their lungs. Mice immunized with the combined DNA vaccines were shown to limit the growth of tubercle bacilli both in lungs and in spleens. Histopathological analyses showed that vaccinated mice had substantially improved postinfection lung pathology relative to the controls. We suggest that our combination of antigens together with DDA formulation may provide a new insight into tuberculosis prevention.     

结球甘蓝和青花菜小孢子胚植株再生

结球甘蓝（Brassica oleracea var.capitata）和青花菜（Brassica oleracea var.italica）小孢子胚再生植株频率低是目前影响游离小孢子培养技术有效应用的关键问题之一,研究其小孢子胚植株再生频率的影响因素,提高胚再生植株频率,对促进游离小孢子培养技术在甘蓝类蔬菜育种中更好地应用具有重要意义。该文以结球甘蓝中甘11和青花菜TI-111等基因型为试材,对影响游离小孢子胚再生成植株的固体培养基类型、琼脂浓度、胚的类型及胚在液体培养基中的滞留时间等因素进行了研究。结果表明：游离小孢子培养25天的子叶胚在琼脂浓度为1%–1.25%的B5培养基上植株再生频率最高。进一步通过8个不同基因型对上述实验结果进行了验证,结果显示,游离小孢子培养25天的子叶胚在1%琼脂浓度的B5培养基上植株再生频率达77.8%–97.2%。     

Production and purification of a novel thermostable phytase by Pichia pastoris FPHY34

A genetically engineered Pichia pastoris FPHY34 strain containing a 1.3 kb thermostable phytase gene (fphy) evolved by DNA shuffling was constructed and screened. Expression and purification conditions for the recombinant phytase were developed in this study. The effect of Pi on recombinant phytase expression and cell growth of P. pastoris FPHY34 was tested in shake flask culture. Optimization of carbon sources for cell growth and methanol feeding strategies for phytase expression in P. pastoris FPHY34 was carried out in a 50-L fermenter by fed-batch fermentation. The purification of phytase was investigated by micro-filtration and ultra-filtration followed by desalting, ion-exchange chromatography, and gel filtration in the ÄKTA system. It showed that the optimum inorganic phosphorus is 13.6 g L⁻¹ and that glucose can be used as a substrate for P. pastoris cell growth instead of glycerol; the biomass yield of glycerol (Y_X/S) is slightly higher than that of glucose. Different profiles of lag phase and respiratory quotient (RQ) displayed between glucose and glycerol as the sole carbon source. The maximum phytase activity in per millimetre reached 2508 U mL⁻¹ at a methanol feed rate of 3.0 mL L⁻¹ h⁻¹ after 80 h period of induction. A purification factor of 41.1 with a 32% yield was achieved after chromatographic purification. The specific enzyme activity was 80 U mg⁻¹ and 3281 U mg⁻¹ in that supernatant fraction and after gel filtration purification, respectively. The strain P. pastoris FPHY34 showed a promising application in phytase industrial production.     

芽胞杆菌生产嘌呤核苷研究进展

嘌呤核苷在食品和医药领域都有广泛的应用。应用芽胞杆菌生产嘌呤核苷类物质的研究始于 6 0年代 ,其后对合成途径及其代谢调控机制相继进行了深入的研究 ,以下就此做详尽的综述 ,并以肌苷、鸟苷为例 ,对利用芽胞杆菌发酵生产嘌呤核苷的菌种选育和工艺调控的进展做全面的回顾。     

射频消融联合吉非替尼治疗晚期非小细胞肺癌的临床分析

目的:观察射频消融术(RFA)联合吉非替尼(Gefitinib)对晚期非小细胞肺癌(NSCLC)治疗的临床效果。方法:26例晚期NSCLC(III、IV期)患者用RFA联合Gefitinib治疗,与23例单独使用RFA治疗的对照组进行比较,观察两组之间有效率、生活质量和不良反应。结果:根据疗效、生活质量评分等指标进行评价,联合治疗组有效率为65.4%,高于单纯RFA治疗组的34.8%,两组之间有显著性差异(P<0.01),对接受治疗的患者进行生活质量评价结果表明,所有接受RFA和Gefitinib联合治疗组中有71.3%的病人生活质量良好,而接受单纯RFA治疗组中只有47%的患者生活质量良好,两组之间有显著性差异(P<0.01),两组患者不良反应主要是疼痛、发热、腹泻等,只需对症处理。结论:RFA联合Gefitinib综合治疗NSCLC方案优于单纯采用RFA的治疗方案。     

帕金森病丘脑底核神经元的电活动特点

本研究探讨了帕金森病(Parkinson′s disease, PD)患者丘脑底核(subthalamic nucleus, STN)神经元电活动的特点及其与PD症状的关系. 35例PD患者在接受手术治疗的同时, 应用微电极细胞记录和EMG记录技术, 记录手术靶点STN及其周围结构神经元的电活动以及手术对侧肢体的EMG. 应用分析软件甄别单细胞电活动, 分析其特点及其与肢体EMG的关系. 结果表明, STN及其周围结构具有特征性放电活动.在36个记录针道中, 共发现436个STN神经元, 平均放电频率44.0±20.5 Hz. 其中, 56%的神经元呈不规则簇状放电; 15%呈紧张性放电; 29%呈规则的簇状放电, 其放电节律与肢体震颤的EMG高度一致(r2=0.66, P<0.01), 称之为震颤细胞. 在PD震颤型患者的STN中发现大量震颤细胞, 且80%位于STN中上部, 而在PD僵直型患者的STN中均发现与运动相关的细胞电活动. 本研究提示, 通过微电极记录技术可准确地判断STN的位置和范围; 与震颤活动相关的细胞放电和与运动相关细胞的放电与PD症状有内在关系; STN参与PD运动障碍的病理生理过程.     

中药柴胡不同采收期的皂甙含量

中药柴胡不同采收期的皂甙含量潘泽惠庄体德周雪林林湘（江苏省中国科学院植物研究所,南京２１００１４）（黑龙江双鸭山矿务局师范学校,双鸭山１５５１２５）ＯｎｓｅａｓｏｎａｌｃｈａｎｇｅｓｏｆｔｈｅｔｏｔａｌｓａｐｏｎｉｎｓｉｎＣｈｉｎｅｓｅｔｒａｄｉｔ...