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291.
292.
Sensitive determination of bromhexine hydrochloride based on its quenching effect on luminol/H2O2 electrochemiluminescence system 下载免费PDF全文
Dexian Kong Siqin Huang Jiansong Cheng Qizhao Zhuang Yijun Liu Chun‐Hua Lu 《Luminescence》2018,33(4):698-703
In this paper, the electrochemiluminescence (ECL) behavior of luminol/H2O2 system in the presence of bromhexine hydrochloride (BrH) was investigated. It was found that the ECL intensity of luminol/H2O2 system on a platinum electrode could be intensely quenched by BrH owing to the scavenging superoxide radical ability of BrH, and therefore the sensitive determination of BrH was possible. Under optimal conditions, the quenched ECL intensity was linear to the concentration of BrH in a wide range of 0.08 to 500 μM, with a detection limit of 0.02 μM (signal‐to‐noise ratio (S/N) = 3). This ECL method possessed the merits of rapid, simple and sensitive, and was successfully applied to the BrH quantification in pharmaceutical preparations with satisfactory recoveries of 91.0 ± 4.0 to 106.5 ± 3.4%. The possible route of the quenched ECL of luminol/H2O2 in the presence of BrH was also discussed. 相似文献
293.
Fang Wang Bei Liu Zhou Yu Tong Wang Yajuan Song Ran Zhuang Yonghong Wu Yingjun Su Shuzhong Guo 《Journal of molecular histology》2018,49(3):277-287
Diabetes is a condition that causes delayed wound healing and results in chronic wounds. CD100 has been reported to promote and induce potent and obvious angiogenesis both in vivo and in vitro studies, the absence of which are a main cause of the diabetic chronic wound. In the present study, we investigated the effects of application of soluble CD100 on wound healing in diabetic mice. Four 5-mm full-thickness dermal wounds were made on each male db/db mouse. 12 mice from CD100 group were subcutaneously injected with 250 ng of CD100 (50 µl) per wound, in addition, 12 mice were injected with the same volume phosphate-balanced solution as the control. The animals were treated every other day until the wounds healed completely. Images were obtained to calculate the area ratio of the original area. HE and Masson’s trichrome staining were used for histological examination. Collagen remodeling, angiogenesis and wound bed inflammation were evaluated by immunohistochemical staining and western blot. We demonstrated that CD100 had distinct functions during the wound healing process. Histological and western blotting analysis showed a more organized epithelium and dermis, more collagen fibers, higher angiogenesis and lower inflammation in the CD100 group than in the PBS group. These findings suggest that CD100 may accelerate wound healing in diabetic mice by promoting angiogenesis in the wound and by reducing the inflammatory response. 相似文献
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Repression of microRNA‐382 inhibits glomerular mesangial cell proliferation and extracellular matrix accumulation via FoxO1 in mice with diabetic nephropathy 下载免费PDF全文
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Silencing of SOCS‐1 and SOCS‐3 suppresses renal interstitial fibrosis by alleviating renal tubular damage in a rat model of hydronephrosis 下载免费PDF全文
297.
Effects of long noncoding RNA SPRY4‐IT1‐mediated EZH2 on the invasion and migration of lung adenocarcinoma 下载免费PDF全文
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Comparative performance of different scale‐down simulators of substrate gradients in Penicillium chrysogenum cultures: the need of a biological systems response analysis 下载免费PDF全文
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Evolution and characterisation of the AhRAF4 NB‐ARC gene family induced by Aspergillus flavus inoculation and abiotic stresses in peanut 下载免费PDF全文
Y. Deng H. Chen C. Zhang T. Cai B. Zhang S. Zhou J. C. Fountain R.‐L. Pan B. Guo W.‐J. Zhuang 《Plant biology (Stuttgart, Germany)》2018,20(4):737-750
- Aflatoxin contamination in peanut is a serious food safety issue to human health around the world. Finding disease resistance genes is a key strategy for genetic improvement in breeding to deal with this issue.
- We identified an Aspergillus flavus‐induced NBS‐LRR gene, AhRAF4, using a microarray‐based approach. By comparison of 23 sequences from three species using phytogenetics, protein secondary structure and three‐dimensional structural analyses, AhRAF4 was revealed to be derived from Arachis duranensis by recombination, and has newly evolved into a family of several members, characterised by duplications and point mutations. However, the members of the family descended from A. ipaensis were lost following tetraploidisation.
- AhRAF4 was slightly up‐regulated by low temperature, drought, salicylic acid and ethylene, but down‐regulated by methyl jasmonate. The distinct responses upon As. flavus inoculation and the differential reactions between resistant and susceptible varieties indicate that AhRAF4 might play a role in defence responses. Temporal and spatial expression and the phenotype of transformed protoplasts suggest that AhRAF4 may also be associated with pericarp development.
- Because tetraploid cultivated peanuts are vulnerable to many pathogens, an exploration of R‐genes may provide an effective method for genetic improvement of peanut cultivars.