排序方式: 共有78条查询结果,搜索用时 379 毫秒
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A full-length cDNA encoding a dehydrin was cloned from the living fossil plant Ginkgo biloba by rapid amplification of cDNA ends (RACE). The cDNA, designated as GbDHN, was 813 bp long containing an open reading frame of 489 bp. The deduced GbDHN protein had 163 amino acid residues, which formed a 17 kDa polypeptide with a predicted isoelectric point (pI) of 5.75. GbDHN had an S-segment and a K-segment, indicative of dehydrins, but no Y-segments. Homology analysis indicated that the S-segment and K-segment of GbDHN shared identity with those of other reported dehydrins, indicating that GbDHN belonged to dehydrin superfamily. Genomic sequence of GbDHN was also cloned using genomic walker technology. By comparing genomic DNA with the cDNA, it was found that there was a 257-bp intron in this gene. Promoter analysis indicated that it contained six CAAT boxes, one TATA box, one ABRE box and one GC-motif in the 5′-flanking region. Southern blot analysis revealed that GbDHN belonged to a single copy gene family. RT-PCR analysis revealed that GbDHN constitutively expressed in stems and roots. The increased expression of GbDHN was detected when G. biloba seedlings were treated with exogenous abscisic acid (ABA), salt stress and drought stress. These results indicate that the GbDHN has the potential to play a role in response to ABA and environmental stresses that can cause plant dehydration. 相似文献
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Adenoviral-vector mediated transfer of HBV-targeted ribonuclease can inhibit HBV replication in vivo
Zhao Y Li Y Liu J Liu Z Huang Y Lei J Li S Xue C 《Biochemical and biophysical research communications》2008,371(3):541-545
Hepatitis B virus (HBV)-targeted ribonuclease (HBV-TR) is a fused protein of HBV core protein and a ribonuclease, human eosinophil-derived neurotoxin (hEDN). Our previous results showed that HBV-TR could effectively inhibit HBV replication in vitro. To test whether HBV-TR can inhibit HBV replication in vivo, we constructed a recombinant adenoviral vector expressing HBV-TR (Ad-TR) and used it to treat HBV-transgenic mice. Immunohistochemical staining showed that TR was expressed at varied levels in different tissues of Ad-TR-treated mice. Serum HBsAg concentration was decreased by 64.8% for the Ad-TR-treated mice compared with empty adenoviral vector-treated control mice. The amount of HBV-DNA in the livers of the Ad-TR-treated mice was 0.74 × 107 copies/μg of genomic DNA while the amount of HBV-DNA in the livers of the empty adenoviral vector-treated control mice was 2.86 × 107 copies/μg of genomic DNA. Serum HBV-DNA of Ad-TR-treated mice was also decreased by 71.4% compared with empty adenoviral vector-treated control mice. In addition, for some Ad-TR-treated mice, the expression of HBsAg in the liver cells turned negative. No discernible adverse effects were observed for Ad-TR-treated mice. Taken together, our results indicated that adenovirus mediated transfer of HBV-TR can inhibit HBV replication in vivo. 相似文献
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Sergeeva MV Zhou Y Bartkowski DM Nolan TG Norris DA Okamoto E Kirkovsky L Kamran R Lebrun LA Tsan M Patel R Shah AM Lardy M Gobbi A Li LS Zhao J Bertolini T Stankovic N Sun Z Murphy DE Webber SE Dragovich PS 《Bioorganic & medicinal chemistry letters》2008,18(11):3421-3426
5-Hydroxy-3(2H)-pyridazinone derivatives were investigated as potent inhibitors of genotype 1 HCV NS5B polymerase focusing on the optimization of their drug metabolism and pharmacokinetics (DMPK) profiles. This investigation led to the discovery of potent inhibitors with improved DMPK properties. 相似文献
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Li LS Zhou Y Murphy DE Stankovic N Zhao J Dragovich PS Bertolini T Sun Z Ayida B Tran CV Ruebsam F Webber SE Shah AM Tsan M Showalter RE Patel R Lebrun LA Bartkowski DM Nolan TG Norris DA Kamran R Brooks J Sergeeva MV Kirkovsky L Zhao Q Kissinger CR 《Bioorganic & medicinal chemistry letters》2008,18(11):3446-3455
5-Hydroxy-3(2H)-pyridazinone derivatives were investigated as inhibitors of genotype 1 HCV NS5B polymerase. Lead optimization led to the discovery of compound 3a, which displayed potent inhibitory activities in biochemical and replicon assays [IC(50) (1b)<10nM; IC(50) (1a)=22 nM; EC(50) (1b)=5nM], good stability toward human liver microsomes (HLM t(1/2)>60 min), and high ratios of liver to plasma concentrations 12h after a single oral administration to rats. 相似文献
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Zhou Y Webber SE Murphy DE Li LS Dragovich PS Tran CV Sun Z Ruebsam F Shah AM Tsan M Showalter RE Patel R Li B Zhao Q Han Q Hermann T Kissinger CR Lebrun L Sergeeva MV Kirkovsky L 《Bioorganic & medicinal chemistry letters》2008,18(4):1413-1418
5-Hydroxy-3(2H)-pyridazinone derivatives were investigated as inhibitors of genotype 1 HCV NS5B polymerase. The synthesis, structure-activity relationships (SAR), metabolic stability, and structure-based design approach for this new class of compounds are discussed. 相似文献
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于2009年5-7月定点采集了江苏海域绿潮藻类,测定、分析了这些藻类核糖体rDNA ITS序列,并进行分类鉴定。研究结果显示,ITS+5.8S序列片段长度为552-578 bp,其中ITS1序列部分长度为179-182 bp,5.8S序列全长为155-158 bp,ITS2序列全长为180-196 bp,序列的平均GC含量(contents)为61.6%-63.3%,不同ITS序列存在不同的插入/缺失位点。扩增得到27个序列中有7个不同序列,依据NCBI数据库相似性查找、系统进化关系及遗传距离等分析结果,确定有4个种类:浒苔(Ulva prolifeya),缘管浒苔(U.linza),石莼属(Ulva sp.)1种及盘苔属(Blidingia sp.)1种。分析表明,ITS序列可作为石莼科种类鉴定的标记。 相似文献
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Zhiwei Huang Xin Kuang Zhongxiang Chen Zhijia Fang Song Wang Ping Shi 《Current microbiology》2014,68(4):448-456
Chromium is a significant mutagen and carcinogen in environment. We compared the effects of tri- and hexavalent chromium on cytotoxicity and oxidative stress in yeast. Cell growth was inhibited by Cr3+ or Cr6+, and Cr6+ significantly increased the lethal rate compared with Cr3+. Both Cr3+ and Cr6+ can enter into the yeast cells. The percent of propidium iodide permeable cells treated with Cr3+ is almost five times as that treated with the same concentration of Cr6+. Levels of TBARS, O2 ?, and carbonyl protein were significantly increased in both Cr6+- and Cr3+-treated cells in a concentration- and time-dependent manner. Moreover, the accumulation of these stress markers in Cr6+-treated cells was over the Cr3+-treated ones. The decreased GSH level and increased activity of GPx were observed after 300 μM Cr6+-exposure compared with the untreated control, whereas there was no other change of GSH content in cells treated with Cr3+ even at very high concentration. Exposure to both Cr3+ and Cr6+ resulted in the decrease of activities of SOD and catalase. Furthermore, the effect of Cr6+ is stronger than that of Cr3+. Null mutation sensitivity assay demonstrated that the gsh1 mutant was sensitive to Cr6+ other than Cr3+, the apn1 mutant is more sensitive to Cr6+ than Cr3+, and the rad1 mutant is sensitive to both Cr6+ and Cr3+. Therefore, Cr3+ can be concluded to inhibit cell growth probably due to the damage of plasma membrane integrality in yeast. Although both tri- and hexavalent chromium can induce cytotoxicity and oxidative stress, the action mode of Cr3+ is different from that of Cr6+, and serious membrane damage caused by Cr3+ is not the direct consequence of the increase of lipid peroxidation. 相似文献