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821.
The Chinese species of the genera Omicrogiton Orchymont, 1919, Peratogonus Sharp, 1884 and Mircogioton Orchymont, 1937 are reviewed, diagnosed and keyed. Mircogioton and Omicrogiton are reported for the first time from China, Peratogonus for the first time for mainland China. Five species are recognized: Omicrogiton
coomani Balfour-Browne, 1939 (Guangdong, Hongkong), Omicrogiton
hainanensis
sp. n. (Hainan), Omicrogiton
roberti
sp. n. (Hainan), Mircogioton
coomani Orchymont, 1937 (Yunnan), and Peratogonus
reversus Sharp, 1884 (Guangdong, Jiangxi, Taiwan). Lectotype of Omicrogiton
coomani is designated. Mircogioton
cognitus (Malcolm, 1981), syn. n. is considered a junior subjective synonym of Mircogioton
coomani Orchymont, 1939. Species of Mircogioton and Omicrogiton inhabit decaying banana trunks, whereas Peratogonus
reversus was always collected from moist forest leaf litter. 相似文献
822.
Liguo Feng Han Ding Jia Wang Meng Wang Wei Xia Shu Zang Lixia Sheng 《Saudi Journal of Biological Sciences》2015,22(4):417-423
Salt stress is one important factor influencing the growth and development of plants, and salt tolerance of plants is a result of combined action of multiple genes and mechanisms. Rosa rugosa is not only an important ornamental plant, but also the natural aromatic plant of high value. Wild R. rugosa which is naturally distributed on the coast and islands of China has a good salt tolerance due to the special living environment. Here, the vacuolar Na+/H+ reverse transporter gene (NHX1) and the vacuolar H+-ATPase subunit C gene (VHA-c) closely related to plant salt tolerance were isolated from wild R. rugosa, and the expression patterns in R. rugosa leaves of the two genes under NaCl stress were determined by real-time quantitative fluorescence PCR. The results showed that the RrNHX1 protein is a constitutive Na+/H+ reverse transporter, the expression of the RrNHX1 gene first increased and then decreased with the increasing salt concentration, and had a time-controlled effect. The RrVHA-c gene is suggestive of the housekeeping feature, its expression pattern showed a similar variation trend with the RrNHX1 gene under the stress of different concentrations of NaCl, and its temporal expression level under 200 mM NaCl stress presented bimodal change. These findings indicated that RrNHX1 and RrVHA-c genes are closely associated with the salt tolerance trait of wild R. rugosa. 相似文献
823.
Baoge Qu Yiguo Jia Yuanxun Liu Hui Wang Guangying Ren Hong Wang 《Cell stress & chaperones》2015,20(6):885-892
As an intracellular polypeptide, heat shock protein 70 (HSP70) can be exposed on the plasma membrane and/or released into the circulation. However, the role of HSP70 in various nondisease and disease conditions remains unknown. Quantitative methods for the detection of HSP70 have been used in clinical studies, revealing that an increase in circulating HSP70 is associated with various types of exercise, elderly patients presenting with inflammation, mobile phones, inflammation, sepsis, chronic obstructive pulmonary disease, asthma, carotid intima-media thickness, glutamine-treated ill patients, mortality, diabetes mellitus, active chronic glomerulonephritis, and cancers. Circulating HSP70 decreases with age in humans and in obstructive sleep apnea, arteriosclerosis, atrial fibrillation (AF) following coronary artery bypass surgery, nonalcoholic fatty liver disease, moderate-to-severe alcoholic fatty liver disease, hepatic steatosis, and Helicobacter pylori infection. In conclusion, quantitative methods can be used to detect HSP70, particularly in determining circulating HSP70 levels, using more convenient and rapid screening methods. Studies have shown that changes in HSP70 are associated with various nondisease and disease conditions; thus, HSP70 might be a novel potential biomarker reflecting various nondisease conditions and also the severity of disease conditions. However, the reliability and accuracy, as well as the underlying mechanism, of this relationship remain poorly understood, and large-sample clinical research must be performed to verify the role.
Electronic supplementary material
The online version of this article (doi:10.1007/s12192-015-0618-8) contains supplementary material, which is available to authorized users. 相似文献824.
Hongcheng Zhu Xi Yang Jia Liu Lu Zhou Chi Zhang Liping Xu Qin Qin Liangliang Zhan Jing Lu Hongyan Cheng Xinchen Sun 《Cell stress & chaperones》2015,20(2):217-220
Eukaryotic elongation factor 2 (eEF2) is a member of the GTP-binding translation elongation factor family that is essential for protein synthesis. eEF2 kinase (eEF2K) is a structurally and functionally unique protein kinase in the calmodulin-mediated signaling pathway. eEF2K phosphorylates eEF2, thereby inhibiting eEF2 function under stressful conditions. eEF2K regulates numerous processes, such as protein synthesis, cell cycle progression, and induction of autophagy and apoptosis in cancer cells. This review will demonstrate the mechanisms underlying eEF2K activity in cancer cells under different stresses, such as nutrient deprivation, hypoxia, and DNA damage via eEF2 regulation. In vivo, in vitro, and clinical studies indicated that eEF2K may be a novel biomarker and therapeutic target for cancer. 相似文献
825.
826.
827.
Xian Jia Shuyu Liu Hao Zheng Bo Li Qi Qi Lei Wei Taiyi Zhao Jian He Jingchen Sun 《BMC genomics》2015,16(1)
Background
The analysis of codon usage is a good way to understand the genetic and evolutionary characteristics of an organism. However, there are only a few reports related with the codon usage of the domesticated silkworm, Bombyx mori (B. mori). Hence, the codon usage of B. mori was analyzed here to reveal the constraint factors and it could be helpful to improve the bioreactor based on B. mori.Results
A total of 1,097 annotated mRNA sequences from B. mori were analyzed, revealing there is only a weak codon bias. It also shows that the gene expression level is related to the GC content, and the amino acids with higher general average hydropathicity (GRAVY) and aromaticity (Aromo). And the genes on the primary axis are strongly positively correlated with the GC content, and GC3s. Meanwhile, the effective number of codons (ENc) is strongly correlated with codon adaptation index (CAI), gene length, and Aromo values. However, the ENc values are correlated with the second axis, which indicates that the codon usage in B. mori is affected by not only mutation pressure and natural selection, but also nucleotide composition and the gene expression level. It is also associated with Aromo values, and gene length. Additionally, B. mori has a greater relative discrepancy in codon preferences with Drosophila melanogaster (D. melanogaster) or Saccharomyces cerevisiae (S. cerevisiae) than with Arabidopsis thaliana (A. thaliana), Escherichia coli (E. coli), or Caenorhabditis elegans (C. elegans).Conclusions
The codon usage bias in B. mori is relatively weak, and many influence factors are found here, such as nucleotide composition, mutation pressure, natural selection, and expression level. Additionally, it is also associated with Aromo values, and gene length. Among them, natural selection might play a major role. Moreover, the “optimal codons” of B. mori are all encoded by G and C, which provides useful information for enhancing the gene expression in B. mori through codon optimization.Electronic supplementary material
The online version of this article (doi:10.1186/s12864-015-1596-z) contains supplementary material, which is available to authorized users. 相似文献828.
Yao YW Shi Y Jia ZF Jiang YH Gu Z Wang J Aljofan M Sun ZG 《Histochemistry and cell biology》2011,136(2):205-215
To investigate the biological significance of ubiquitin carboxyl-terminal hydrolase L1 (UCH-L1) involvement in oocyte maturation,
we screened for proteins that bound to UCH-L1 in mouse ovaries, and we found that the prostate tumor overexpressed-1 (PTOV1)
protein was able to bind to UCH-L1. PTOV1 is highly expressed in prostate cancers and considered as a potential marker for
carcinogenesis and the progress of prostate cancer. It was reported that PTOV1 plays an important role in cell cycle regulation,
but its role in mammalian oocyte development and meiosis is still unclear. In this paper, it was found that the expression
levels of PTOV1 in mouse ovaries progressively increased from prepubescence to adulthood. And we found by immunohistochemistry
that PTOV1 spreaded in both the cytoplasm and nuclei of oocytes during prepuberty, but in normal adult mouse oocytes, it concentrated
not only in nuclei but also on the plasma membrane, though in some oocytes with abnormal shapes, PTOV1 did not display the
typical distribution patterns. In granulosa cells, however, it was found to locate in the cytoplasm at all the selected ages.
In postnatal mouse ovaries (28 days), estradiol treatment induced the adult-specific distribution pattern of PTOV1 in oocytes.
In addition, UCH-L1 was shown to be associated with CDK1, which participated in the regulation of cell cycle and oocyte maturation.
Therefore, we propose that the distribution changes of PTOV1 are age-dependent, and significant for mouse oocyte development
and maturation. Moreover, the discovery that PTOV1 is associated with UCH-L1 in mouse oocytes supports the explanations for
that UCH-L1 is involved in oocyte development and maturation, especially under the regulation of estrogen. 相似文献
829.
Wang H Sun Y Liu S Yu H Li W Zeng J Chen C Jia J 《FEMS immunology and medical microbiology》2011,63(1):82-92
Helicobacter pylori is a major human pathogen associated with gastric diseases such as chronic active gastritis, peptic ulcer, and gastric carcinoma. The growth factor progranulin (PGRN) is a secreted glycoprotein that functions as an important regulator of cell growth, migration, and transformation. We aimed to determine the molecular mechanisms by which H. pylori upregulates the expression of PGRN and the relationship between H. pylori infection and production of PGRN in controlling cell proliferation and migration. Levels of PGRN were examined in gastric tissues from patients and in vitro in gastric epithelial cells. Cell proliferation was measured by colony formation assay. Cell migration was monitored by wound healing migration assay. PGRN protein levels were increased in patients with gastritis and gastric cancer tissue. Infection of gastric epithelial cells with H. pylori significantly increased PGRN expression in a time-dependent manner. Blockade of the p38 and MEK1/2 pathway by inhibitor inhibited H. pylori-mediated PGRN upregulation. Activation of p38 and MEK1/2 pathway by H. pylori was also identified. Knockdown of PGRN attenuated the H. pylori-induced proliferative activity and migration of cancer cells. These findings suggest that the upregulation of PGRN in H. pylori-infected gastric epithelial cells may contribute to the carcinogenic process. 相似文献
830.
Guanghai Yu Xiaoqiang Jia Jianping Wen Guoying Wang Yunlin Chen 《World journal of microbiology & biotechnology》2011,27(8):1859-1868
The effects of eight cofactors of enzymes on daptomycin production were investigated in this work, which included nicotinic
acid (VPP), riboflavin (VB2), heme, thiamine (VB1), biotin (VH), cyanocobalamin (VB12), tetrahydrofolic acid (THF) and pyridoxal 5-phosphate (VB6). The dry cell weight (DCW), consumption of glucose, and daptomycin production were obviously improved when proper amount
of exogenous cofactors were supplemented in the medium. The effects of heme, THF, VB12 and VB6 on daptomycin production were especially notable. The daptomycin yield enhanced 363, 104, 53 and 46%, respectively, when
optimized amount of these four cofactors were supplemented in the broth. Moreover, the daptomycin yield further increased
to 632 mg/l, which was over 4.5-fold higher than that of the control (without cofactors), at 132 h in a 7.5-l fermenter, by
supplementation all of the eight cofactors at optimized concentrations (VPP 4 mg/l, VB2 0.5 mg/l, heme 9 mg/l, VB1 0.4 mg/l, VH 0.1 mg/l, VB12 0.04 mg/l, THF 6 mg/l and VB6 0.4 mg/l). Further, the effects of cofactors on the corresponding key enzymes and important intracellular metabolites were
studied in order to elucidate the mechanism of enhancement of daptomycin production by manipulation of cofactors concentration
in the fermentation culture. It is suggested that this strategy for increasing the daptomycin production in Streptomyces roseosporus LC-51 by manipulation of cofactors concentration in the fermentation culture may provide an alternative approach to enhance
the production of metabolites in other Streptomyces. 相似文献