高产融合子Q4413的形态学与生理生化特征的研究

本文通过对枯草芽孢杆菌BR151衍生株与北京棒杆菌1134衍生株的赖氨酸高产融合子Q4413株的形态学、生理生化特性等方面的研究,揭示了融合子与双亲株在这些方面的差异,为Q4413株确系双亲株的重组子或新的融合子增加了佐证.     

由大肠杆菌中提取重组猪生长激素的研究

 本文通过研究提出了一种由大肠杆菌细胞中提取重组猪生长激素的方法。提取回收率为52.4％,纯度为94.1％,具有生物学活性。     

草鱼血液学的研究：Ⅱ.血清电解质和尿素氮的周年变化

本文研究了草鱼稚鱼血清钾、钠、钙、氯、无机磷和尿素氮含量的周年变动规律,分析了水温、体重对上述血液化学成分的影响。结果显示其周年变动无一定规律性可循。血清钠、氯变动辐度较小,无机磷和尿素氮变动幅度较大,幅值约有4倍。水温与血清钾显示正相关(P≤0.05),与钠、钙显示负相关(P≤0.01,P≤0.05)。血清钠、尿素氮有随体重增加含量增高的趋势,而钾却显著减少。     

抑菌生的研究总结报告

本文报告一种由枯草杆菌制成的生态制剂,并命名为抑菌生(Subtilobiogen)。该制剂对创、烧伤感染有治疗作用。经过安全试验、急性毒性试验、Ames试验和微核试验证明,该制剂是一种无害、无毒和有致突变作用的活菌制剂。抑菌生有膏剂、乳剂及粉剂3种剂型。抑菌生在试管和体内对金黄色葡萄球菌、绿脓杆菌和大肠杆菌均具有抑菌作用。临床观察证明,将抑菌生喷洒在创面上,对浅Ⅱ°度、深Ⅱ°度及混合型烧伤感染均具有明显疗效。实验组(181例)与对照组(174例)相比较,在统计学上具有显著性差异。抑菌生的作用机制,经过初步试验证明,与营养争夺和占位性保护有关,因为枯草杆菌的生长速度超过金黄色葡萄球菌、绿脓杆菌和大肠杆菌的生长速度。     

Nucleotide sequences of serine tRNAs from Bacillus subtilis.

Three B. subitilis serine tRNAs were sequenced including modified nucleosides. All the serine tRNAs contained 1-methyl-adenosine in the D-loop. As other characteristic modified nucleosides, 5-methoxyuridine was found in the first letter of the anticodon in the tRNA(UGA).     

Steroidal saponins from Smilax lebrunii.

Two new steroidal saponins, (25 R)-spirostan-3 beta-ol-6-one-3-O-[alpha-L-arabinopyranosyl (1----6)]-beta-D-glucopyranoside and (25 R)-spirostan-3 beta-ol-6-one-3-O-[beta-D-glucopyranosyl(1---4)] [alpha-L-arabinopyranosyl(1----6)]-beta-glucopyranoside, were isolated from the rhizomes of Smilax lebrunii. Their structures have been established by chemical and spectral methods.     

Phenylpropanoid and iridoid glycosides from Pedicularis lasiophrys.

Two new compounds, pedicularioside E and F, were isolated from whole plants of Pedicularis lasiophrys, along with the four known compounds, verbascoside, cistanoside C, cistanoside D and 8-epiloganin. On the basis of spectral and chemical evidence, pedicularioside E and F were identified to be 1'-O-beta-D-(3-methoxy-4-hydroxy-beta-phenyl)-ethyl-6'-O-feruloyl- alpha-L-(2-acetyl)-rhamnosyl-(1----3')-4'-acetylglucopyranoside and shanzhisin methyl ester cellobioside, respectively.     

Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat

Summary Genomic in situ hybridization was used to identify alien chromatin in chromosome spreads of wheat, Triticum aestivum L., lines incorporating chromosomes from Leymus multicaulis (Kar. and Kir.) Tzvelev and Thinopyrum bessarabicum (Savul. and Rayss) Löve, and chromosome arms from Hordeum chilense Roem. and Schult, H. vulgare L. and Secale cereale L. Total genomic DNA from the introgressed alien species was used as a probe, together with excess amounts of unlabelled blocking DNA from wheat, for DNA:DNA in-situ hybridization. The method labelled the alien chromatin yellow-green, while the wheat chromosomes showed only the orange-red fluorescence of the DNA counterstain. Nuclei were screened from seedling root-tips (including those from half-grains) and anther wall tissue. The genomic probing method identified alien chromosomes and chromosome arms and allowed counting in nuclei at all stages of the cell cycle, so complete metaphases were not needed. At prophase or interphase, two labelled domains were visible in most nuclei from disomic lines, while only one labelled domain was visible in monosomic lines. At metaphase, direct visualization of the morphology of the alien chromosome or chromosome segment was possible and allowed identification of the relationship of the alien chromatin to the wheat chromosomes. The genomic in-situ hybridization method is fast, sensitive, accurate and informative. Hence it is likely to be of great value for both cytogenetic analysis and in plant breeding programmes.     

Intra-specific competition and density dependent juvenile growth

A difference equation model for the dynamics of a semelparous size-structured species consisting of juvenile and adult individuals is derived and studied. The adult population consists of two size classes, a smaller class and a larger more fertile class. Negative feedback occurs through slowed juvenile growth due to increased total population levels during the developmental period and consequently a smaller adult size at maturation. Intra-specific competition coefficients are size dependent and measure the strength of intra-specific competition between juveniles and adults. It is shown that equilibrium states in which adults and juveniles occur together at all times are in general destabilized by significantly increased juvenilevs adults competition with the result that stable periodic cycles appear, in which the generations alternate in time and hence avoid competition. This result supports the tenet that intra-specific competition between juveniles and adults is destabilizing. Exceptions to this destabilization principle are found, however, in which populations exhibiting non-equilibrium, aperiodic dynamics can be equilibrated by increase competition between juveniles and adults. This occurs, for example, when adult fertility and competition coefficients are significantly size class dependent. The author gratefully acknowledges the support of the Applied Mathematics Division and the Population Biology/Ecology Division of the National Science Foundation under NSF grant No. DMS-8902508. Research supported by the Department of Energy under contracts W-7405-ENG-36 and KC-07-01-01.     

苜蓿胚状体的分离及其发育过程中三种同工酶酶谱分析(简报)

目前,对胚状体发生过程中的生理生化研究表明,这一过程伴随有核酸、蛋白质等大分子物质合成速度的增加及与胚胎发生有关的特异性蛋白的合成;一些同工酶,如过氧化物酶、脂酶、细胞色素氧化酶和谷氨酸脱氢酶