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91.
Yuan Xu Chen Shao Xinpeng Fan Alan Warren Khaled A. S. Al-Rasheid Weibo Song Norbert Wilbert 《Polar Biology》2016,39(8):1439-1453
Antarctica is a remote and isolated biotope which makes it an ideal location for studying new and endemic species. Since there is little literature available on the diversity of ciliates in this area, a taxonomic survey of ciliates from melt-water of Collins glacier, King George Island, was carried out from January to March 2006. As a result, the morphology and infraciliature of five ciliates, including one new species, are described using live observations and silver staining: Gastronauta multistriata nov. spec., Neokeronopsis asiatica Foissner et al., 2010, Paraholosticha muscicola Kahl, 1932, Oxytricha sp., and Cyclidium glaucoma Müller, 1786. Gastronauta multistriata nov. spec. is distinguished from its congeners by the following combination of characters: cell size in vivo on average 80 × 40 μm; 5–9 kineties in left ciliary field; 18–23 kineties in right ciliary field, including 10–12 postoral kineties; 7–10 preoral kineties; dorsal brush along anterior dorsal margin, consisting of 5–8 groups of basal bodies. The only minor differences between the current population of N. asiatica and a previously described Antarctic population are the numbers of caudal cirri (6–10 vs. 8–15) and dorsal kineties (11–13 vs. 12–18). Paraholosticha sterkii is synonymised with P. muscicola. The Antarctic population of C. glaucoma corresponds well with a former population from China, the only difference being the number of kinetids in SK n (11–17 vs. 9–11). This work will contribute to the understanding of ciliate diversity in this little studied area. 相似文献
92.
Construction of a high density integrated genetic map for cucumber (Cucumis sativus L.) 总被引:1,自引:0,他引:1
Zhang WW Pan JS He HL Zhang C Li Z Zhao JL Yuan XJ Zhu LH Huang SW Cai R 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2012,124(2):249-259
The high-density consensus map was constructed based on the GY14 × PI 183967 map from an inter-subspecific cross and the extended
S94 × S06 map from an intra-subspecific cross. The consensus map was composed of 1,369 loci, including 1,152 SSR loci, 192
SRAP loci, 21 SCAR loci and one STS locus as well as three gene loci of fruit external quality traits in seven chromosomes,
and spanned 700.5 cM, of which 682.7 cM (97.5%) were covered by SSR markers. The average genetic distance and physical interval
between loci were 0.51 cM and ~268 kbp, respectively. Additionally, the physical position of the sequence-associated markers
aligned along the assembled cucumber genome sequence established a relationship between genetic maps and cucumber genome sequence
and to a great extent validated the order of markers in individual maps and consensus map. This consensus map with a high
marker density and well-ordered markers is a saturated and reliable linkage map for genetic analysis of cucumber or the Cucurbitaceae
family of plants. 相似文献
93.
ITMSQ: A software tool for N‐ and C‐terminal fragment ion pairs based isobaric tandem mass spectrometry quantification 下载免费PDF全文
Li‐Qi Xie Lei Zhang Ai‐Ying Nie Guo‐Quan Yan Jun Yao Yang Zhang Peng‐Yuan Yang Hao‐Jie Lu 《Proteomics》2015,15(22):3755-3764
Tandem MS (MS2) quantification using the series of N‐ and C‐terminal fragment ion pairs generated from isobaric‐labelled peptides was recently considered an accurate strategy in quantitative proteomics. However, the presence of multiplexed terminal fragment ion in MS2 spectra may reduce the efficiency of peptide identification, resulting in lower identification scores or even incorrect assignments. To address this issue, we developed a quantitative software tool, denoted isobaric tandem MS quantification (ITMSQ), to improve N‐ and C‐terminal fragment ion pairs based isobaric MS2 quantification. A spectrum splitting module was designed to separate the MS2 spectra from different samples, increasing the accuracy of both identification and quantification. ITMSQ offers a convenient interface through which parameters can be changed along with the labelling method, and the result files and all of the intermediate files can be exported. We performed an analysis of in vivo terminal amino acid labelling labelled HeLa samples and found that the numbers of quantified proteins and peptides increased by 13.64 and 27.52% after spectrum splitting, respectively. In conclusion, ITMSQ provides an accurate and reliable quantitative solutionfor N‐ and C‐terminal fragment ion pairs based isobaric MS2 quantitative methods. 相似文献
94.
Yuanyuan Yu Lei Hou Xu Shi Xiaoru Sun Xinhui Liu Yifan Yu Zhongshang Yuan Hongkai Li Fuzhong Xue 《PLoS genetics》2022,18(3)
Nonrandom selection in one-sample Mendelian Randomization (MR) results in biased estimates and inflated type I error rates only when the selection effects are sufficiently large. In two-sample MR, the different selection mechanisms in two samples may more seriously affect the causal effect estimation. Firstly, we propose sufficient conditions for causal effect invariance under different selection mechanisms using two-sample MR methods. In the simulation study, we consider 49 possible selection mechanisms in two-sample MR, which depend on genetic variants (G), exposures (X), outcomes (Y) and their combination. We further compare eight pleiotropy-robust methods under different selection mechanisms. Results of simulation reveal that nonrandom selection in sample II has a larger influence on biases and type I error rates than those in sample I. Furthermore, selections depending on X+Y, G+Y, or G+X+Y in sample II lead to larger biases than other selection mechanisms. Notably, when selection depends on Y, bias of causal estimation for non-zero causal effect is larger than that for null causal effect. Especially, the mode based estimate has the largest standard errors among the eight methods. In the absence of pleiotropy, selections depending on Y or G in sample II show nearly unbiased causal effect estimations when the casual effect is null. In the scenarios of balanced pleiotropy, all eight MR methods, especially MR-Egger, demonstrate large biases because the nonrandom selections result in the violation of the Instrument Strength Independent of Direct Effect (InSIDE) assumption. When directional pleiotropy exists, nonrandom selections have a severe impact on the eight MR methods. Application demonstrates that the nonrandom selection in sample II (coronary heart disease patients) can magnify the causal effect estimation of obesity on HbA1c levels. In conclusion, nonrandom selection in two-sample MR exacerbates the bias of causal effect estimation for pleiotropy-robust MR methods. 相似文献
95.
96.
该研究以红嘴相思鸟(Leiothrix lutea)为材料,分析对其鸣管神经支(NXIIts)进行断单侧和断双侧处理后的鸣声声学变化,探讨鸣管神经支配特性。结果表明,断单侧NXIIts后红嘴相思鸟均可发出常见叫声,但鸣声音节间隔拉长,音节时程缩短,调频指数下降,且断左侧NXIIts的作用效果明显大于断右侧NXIIts。即NXIIts支配具单侧性,且呈左侧优势,此外,左侧NXIIts还具有产生音节高频成分和谐波的作用。断双侧NXIIts后,红嘴相思鸟鸣声音调唯一,响度大幅下降,音节脉冲数增加。 相似文献
97.
镉离子诱导BA/F3β细胞发生奇特的细胞凋亡 总被引:2,自引:0,他引:2
细胞凋亡一般都伴随有DNA 片段化, 活性氧含量增加, 并能被过量的Bcl2 所抑制。以BA/F3β细胞为模型, 利用MTT 检测、Hochest 染色以及透射电镜检测等技术却发现, 镉离子虽然可以诱导该细胞凋亡, 但是这种凋亡没有DNA 片段化, 也没有活性氧含量增加。此外, 过量Bcl2 对这种凋亡也没有保护作用。因此, 可以确认镉离子诱导BA/F3β细胞发生了奇特的细胞凋亡。 相似文献
98.
Organization and assembly of the TRAPPII complex 总被引:1,自引:0,他引:1
Choi C Davey M Schluter C Pandher P Fang Y Foster LJ Conibear E 《Traffic (Copenhagen, Denmark)》2011,12(6):715-725
Current models suggest that TRAPP tethering complexes exist in two forms. Whereas the seven-subunit TRAPPI complex mediates ER-to-Golgi transport, TRAPPII contains three additional subunits (Trs65, Trs120 and Trs130) and is required for distinct tethering events at Golgi membranes. It is not clear how TRAPPII assembly is regulated. Here, we show that Tca17 is a fourth TRAPPII-specific component, and that Trs65 and Tca17 interact with distinct domains of Trs130 and make different contributions to complex assembly. Whereas Tca17 promotes the stable association of TRAPPII-specific subunits with the core complex, Trs65 stabilizes TRAPPII in an oligomeric form. We show that Trs85, which was previously reported to be a subunit of both TRAPPI and TRAPPII, is not associated with the TRAPPII complex in yeast. However, we find that proteins related to Trs85, Trs65 and Tca17 are part of the same TRAPP complex in mammalian cells. These findings have implications for models of TRAPP complex formation and suggest that TRAPP complexes may be organized differently in yeast and mammals. 相似文献
99.
100.
Yao Shi Juan Yuan Vilma Rraklli Eva Maxymovitz Miriam Cipullo Mingzhi Liu Shuijie Li Isabelle Westerlund Oscar C Bedoya-Reina Petra Bullova Joanna Rorbach C Christofer Juhlin Adam Stenman Catharina Larsson Per Kogner Maureen J OSullivan Susanne Schlisio Johan Holmberg 《Nucleic acids research》2021,49(5):2509