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921.
922.
Feng Shao Yu-Mei Xiong Rong-Huan Zhu Min-Hua Ling Cheng-Wu Chi Da-Cheng Wang 《Protein expression and purification》1999,17(3):358
The gene encoding a neurotoxin (BmK M1) from the scorpion Buthus martensii Karsch was expressed in Saccharomyces cerevisiae at a high level with the alcohol dehydrogenase promoter. SDS–PAGE of the culture confirmed expression and showed secretion into medium from yeast. Recombinant BmK M1 was purified rapidly and efficiently by ion exchange and gel filtration chromatography to homogeneity, produced a single band on tricine–SDS–PAGE, and processed the homologous N-terminus. Amino acid analysis and N-terminal sequencing demonstrated that the recombinant toxin was processed correctly from the α-mating factor leader sequence and was chemically identical to the native form. The expressed recombinant BmK M1 was toxic for mice, which indicated that it was biologically active. Quantitative estimation showed that recombinant BmK M1 had an LD50 similar to that of the native toxin. 相似文献
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924.
The effects of plant litter on vegetation: a meta-analysis 总被引:38,自引:3,他引:35
925.
H. Xiong C. Li E. Garami Y. Wang M. Ramjeesingh K. Galley C.E. Bear 《The Journal of membrane biology》1999,167(3):215-221
ClC-2 belongs to a large family of chloride channels and its expression in certain cell types is associated with the appearance
of swelling-activated chloride (Cl−) currents. In the present report, we examined the hypothesis that ClC-2 plays a role in regulatory volume decrease by expressing
ClC-2 in Sf9 cells using the baculovirus system. First, we showed that ClC-2 protein expression is associated with appearance
of a Cl− conductance which is activated by hypo-osmotic shock and can be distinguished from swelling-activated chloride currents endogenous
to Sf9 cells on the basis of its pharmacology and specific inhibition by an anti-ClC-2 antibody. Second, we show that the
rate of regulatory volume decrease is significantly enhanced in Sf9 cells expressing ClC-2 protein. Hence, our data support
the hypothesis that ClC-2 is capable of mediating regulatory volume decrease.
Received: 12 August/Revised: 23 October 1998 相似文献
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927.
Yaoyao Zhang Xiaodong Sun Zhiwen Nong Mo Chen Yujiang Hao Jianghua Wang Kexiong Wang Ding Wang Zhigang Mei 《Marine Mammal Science》2024,40(2):e13082
The establishment of marine protected areas (MPAs) for cetaceans is an important strategy to mitigate human disturbance and protect biodiversity. Despite abundant cetacean species, there are only a few MPAs dedicated to cetacean conservation in China, all of which are for inshore dolphins. Bryde's whales, the only nearshore baleen whale population in mainland China, are conflicting with intensive human activities, yet an effective conservation strategy is lacking. This study used species distribution models to analyze distribution patterns and suitable habitats of Bryde's whales in the Beibu Gulf and proposes the first baleen whale MPA in China. Our results showed Bryde's whales have a seasonal distribution pattern in the Beibu Gulf, and that the waters around Weizhou Island and the southeastern coast of Vietnam were their core habitats. The seasonal nighttime light data indicated a negative relationship between the number of ship lights and Bryde's whale sightings and suggest that Bryde's whales might be threatened by fisheries. We proposed an MPA based on the results, suggesting that the waters within 20 km around Weizhou Island should be declared a protected area. Furthermore, we recommend that anthropogenic activities in the waters around Weizhou Island are better managed to reduce negative impacts on marine life. 相似文献
928.
Mingchang Zhang Weidong Xu Xuefei Han Huiqing Fan Tao Chen YaXiong Yang Yong Gao Chao Zheng Yi Yang Ting Xiong Yong-Wei Zhang Wee Siang Vincent Lee Weijia Wang Hongge Pan Zhi Gen Yu Junmin Xue 《Liver Transplantation》2024,14(9):2303737
Aqueous zinc ion batteries (ZIBs) exhibit great potential for next-generation energy storage devices. However, significant challenges exist, including the uncontrollable formation of Zn dendrite and side reactions during zinc stripping and plating. The mechanism of Zn dendrite nucleation has yet to be fully understood. In this work, the first principles simulations are used to investigate the Zn dendrite formation process. The unintentionally adsorbed O2− and OH− ions are the inducing factors for Zn dendrite nucleation and growth on the Zn (0001) plane due to significantly increased Zn diffusion barriers. A top-down method is demonstrated to suppress the dendrite using delaminated V2CTx to capture O2− and OH− ions thanks to reduced Zn diffusion barriers. The experimental results revealed significantly suppressed Zn dendrite nucleation and growth, resulting in a layer-by-layer deposit/stripping of Zn. Based on the electrochemical evaluations, the V2CTx-coated Zn composite delivers a high coulombic efficiency of 99.3% at 1.0 mAh cm−2. Furthermore, the full cell achieves excellent cyclic performance of 93.6% capacity retention after 2000 cycles at 1 A g−1. This strategy has broad scalability and can be widely applied in designing metallic anodes for rechargeable batteries. 相似文献
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