全文获取类型
收费全文 | 2785篇 |
免费 | 201篇 |
国内免费 | 150篇 |
专业分类
3136篇 |
出版年
2023年 | 21篇 |
2022年 | 57篇 |
2021年 | 109篇 |
2020年 | 75篇 |
2019年 | 94篇 |
2018年 | 104篇 |
2017年 | 78篇 |
2016年 | 86篇 |
2015年 | 161篇 |
2014年 | 163篇 |
2013年 | 221篇 |
2012年 | 228篇 |
2011年 | 189篇 |
2010年 | 134篇 |
2009年 | 115篇 |
2008年 | 98篇 |
2007年 | 115篇 |
2006年 | 112篇 |
2005年 | 116篇 |
2004年 | 109篇 |
2003年 | 81篇 |
2002年 | 59篇 |
2001年 | 65篇 |
2000年 | 55篇 |
1999年 | 55篇 |
1998年 | 28篇 |
1997年 | 26篇 |
1996年 | 23篇 |
1995年 | 29篇 |
1994年 | 27篇 |
1993年 | 19篇 |
1992年 | 40篇 |
1991年 | 30篇 |
1990年 | 18篇 |
1989年 | 22篇 |
1988年 | 15篇 |
1987年 | 11篇 |
1986年 | 13篇 |
1985年 | 15篇 |
1984年 | 17篇 |
1983年 | 17篇 |
1982年 | 15篇 |
1981年 | 8篇 |
1980年 | 6篇 |
1979年 | 7篇 |
1977年 | 6篇 |
1976年 | 8篇 |
1975年 | 8篇 |
1974年 | 7篇 |
1971年 | 8篇 |
排序方式: 共有3136条查询结果,搜索用时 15 毫秒
81.
82.
83.
84.
RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells 总被引:1,自引:0,他引:1
El-Sibai M Pertz O Pang H Yip SC Lorenz M Symons M Condeelis JS Hahn KM Backer JM 《Experimental cell research》2008,314(7):1540-1552
Rho GTPases are versatile regulators of cell shape that act on the actin cytoskeleton. Studies using Rho GTPase mutants have shown that, in some cells, Rac1 and Cdc42 regulate the formation of lamellipodia and filopodia, respectively at the leading edge, whereas RhoA mediates contraction at the rear of moving cells. However, recent reports have described a zone of RhoA/ROCK activation at the front of cells undergoing motility. In this study, we use a FRET-based RhoA biosensor to show that RhoA activation localizes to the leading edge of EGF-stimulated cells. Inhibition of Rho or ROCK enhanced protrusion, yet markedly inhibited cell motility; these changes correlated with a marked activation of Rac-1 at the cell edge. Surprisingly, whereas EGF-stimulated protrusion in control MTLn3 cells is Rac-independent and Cdc42-dependent, the opposite pattern is observed in MTLn3 cells after inhibition of ROCK. Thus, Rho and ROCK suppress Rac-1 activation at the leading edge, and inhibition of ROCK causes a switch between Cdc42 and Rac-1 as the dominant Rho GTPase driving protrusion in carcinoma cells. These data describe a novel role for Rho in coordinating signaling by Rac and Cdc42. 相似文献
85.
Stéphanie Blanchard Anthony D. William Angeline C.-H. Lee Anders Poulsen Ee Ling Teo Weiping Deng Noah Tu Evelyn Tan Kay Lin Goh Wai Chung Ong Chee Pang Ng Kee Chuan Goh Zahid Bonday Eric T. Sun 《Bioorganic & medicinal chemistry letters》2010,20(8):2443-2447
A series of alkenyl indazoles were synthesized and evaluated in Aurora kinase enzyme assays. Several promising leads were optimized for selectivity towards Aurora B. Excellent binding affinity and good selectivity were achieved with optimized compounds in isolated Aurora subfamily assays. 相似文献
86.
Effect of ecological engineering on the nutrient content of surface sediments in Lake Taihu, China 总被引:1,自引:1,他引:1
Yong-Xia Gao Guang-Wei Zhu Bo-Qiang Qin Yong Pang Zhi-Jun Gong Yun-Lin Zhang 《Ecological Engineering》2009,35(11):1624-1630
Ecological engineering was carried out in Meiliang Bay of Lake Taihu beginning in 2003 in order to improve water quality. There were two main objectives: to improve the growth environment for macrophytes, and to restore macrophyte assemblages. We examined surface sediments once per month beginning in April 2005 to study the response of sediment nutrient content to the ecological engineering. Average total nitrogen (TN) and total phosphorus (TP) concentrations in the surface sediments were 7043 and 1370 mg kg−1, respectively, in May 2005, while after 1 year, TN concentration was reduced to 2929 mg kg−1 and TP concentration was reduced to 352 mg kg−1. We conclude that ecological engineering can lower the nutrient content in surface sediments when it is used to improve water quality. 相似文献
87.
Gang Li Cong-Feng Song Xiao-Mai Pang Yue Yang Jin-Sheng Wang 《Journal of Phytopathology》2009,157(4):208-218
Virulence analysis and restriction fragment length polymorphism (RFLP) were used to evaluated the population structure of Xanthomonas oryzae pv . oryzae ( Xoo ) from the main rice-growing region in China. The pathotype of Xoo was determined for 103 strains by inoculating 13 near-isogenic rice lines using IR24 as the recurrent parent. Sixty-one pathotypes was shared by these strains, on the basis of the consensus of three clustering statistics, and four clusters for pathotype were formed. Cluster 2 consists of strains with high molecular polymorphorism and many pathotypes that are either virulent to a majority of the 13 major resistance ( R ) genes or avirulent only to Xa21 , and is geographically dispersed. The resistance gene Xa21 has broader resistance than others to the strains tested. A probe from a member of the avrBs3/pthA type III effector family, 1376 bp Sph I-digested fragment, was used to screen the genomes of 52 strains tested. Four common bands were found in the DNA fingerprint pattern of Xoo , suggesting basic patterns of evolutionary relationship for members of avrBs3/pthA family and/or the pathogen. Each distinct RFLP banding pattern of each strain was considered as a haplotype; 42 haplotypes were revealed by the probe and divided into four lineages by the same statistics method. It was observed that some isolates with different pathotypes shared the same haplotype and others with different haplotypes harboured identical pathotype. There was a weak correlation between virulent pathotypes and molecular haplotypes. 相似文献
88.
移植骨髓间充质干细胞治疗大鼠糖尿病的研究 总被引:1,自引:0,他引:1
目的 通过移植骨髓间充质干细胞(mesenchymal stem cell,MSC)的方法试治疗大鼠糖尿病。方法 贴壁生长的MSC与大鼠胰腺的细胞共培养以检测其向胰岛细胞分化的潜能。并将体外培养扩增的MSC移植入糖尿病大鼠体内,观测其能否改善糖尿病病情及其在大鼠体内微环境中的分化情况。结果 共培养法可使MSC分化为胰岛样细胞。对大鼠的MSC移植能明显缓解糖尿病病情。结论 MSC移植的方法对大鼠糖尿病有一定的治疗作用。 相似文献
89.
利用免疫组织化学技术研究了永久型热休克蛋白HSC70在白菜花各组织中的分布。结果表明:在正常温度条件下,HSC70在小孢子母细胞、四分体细胞、花药壁绒毡层细胞中分布最多,在花原基、花托的维管组织、花粉母细胞以及发育早期的胚珠中的表达也较多。该结果与其他人用核酸杂交、同位素示踪等技术所得结果基本一致,本文对HSC70在白菜花不同组织中的分布与其功能的关系进行了初步讨论。 相似文献
90.
Pengsheng Chen Sisi Pang Naiquan Yang Haoyu Meng Jia Liu Ningtian Zhou Min Zhang Zhihui Xu Wei Gao Bo Chen Zhengxian Tao Liansheng Wang Zhijian Yang 《PloS one》2013,8(11)
The fruit of Schisandra chinensis has been used in the traditional Chinese medicine for thousands of years. Accumulating evidence suggests that Schisandrin B (Sch B) has cardioprotection effect on myocardial ischemia in
vitro. However, it is unclear whether Sch B has beneficial effects on continuous myocardial ischemia in vivo. The aim of the present study was to investigate whether Sch B could improve cardiac function and attenuate myocardial remodeling after myocardial infarction (MI) in mice. Mice model of MI was established by permanent ligation of the left anterior descending (LAD) coronary artery. Then the MI mice were randomly treated with Sch B or vehicle alone. After treatment for 3 weeks, Sch B could increase survival rate, improve heart function and decrease infarct size compared with vehicle. Moreover, Sch B could down-regulate some inflammatory cytokines, activate eNOS pathway, inhibit cell apoptosis, and enhance cell proliferation. Further in vitro study on H9c2 cells showed similar effects of Sch B on prevention of hypoxia-induced inflammation and cell apoptosis. Taken together, our results demonstrate that Sch B can reduce inflammation, inhibit apoptosis, and improve cardiac function after ischemic injury. It represents a potential novel therapeutic approach for treatment of ischemic heart disease. 相似文献