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排序方式: 共有511条查询结果,搜索用时 656 毫秒
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银叶竹芋的组织培养和快速繁殖 总被引:2,自引:0,他引:2
1植物名称银叶竹芋(Ctenanthe setosa‘Greystar’)。2材料类别当年生茎节。3培养条件芽诱导培养基:(1)MS+BA1mg·L-1(单位下同)+NAA0.1;(2)继代增殖培养基MS+BA6+NAA0.05;(3)生根培养基MS+NAA0.5。以上培养基均附加3%蔗糖(生根培养基中为2%)、0.6%琼脂。pH5.8,培养温度(26±1)℃,每天光照12h,光强40μmol·m-2·s-1。4生长与分化情况4.1无菌材料的获得取当年生茎节,除去根状物,自来水冲洗表面20min。将茎节切成约2cm的小段,每段一芽,在超净工作台上,用75%的酒精浸泡30s,再置于0.1%升汞溶液(加1~2滴吐温20)中消毒10min,然后用无… 相似文献
44.
Shen SS Liu C Xu ZY Hu YH Gao GF Wang SY 《Biochemical and biophysical research communications》2012,420(4):907-912
The mtDNA 1555A>G mutation was considered to be one of the most common causes of aminoglycoside-induced and non-syndromic hearing loss. However, this mutation was always found in homoplasmy with high phenotypic heterogeneity. Recently this mutation in heteroplasmy has been reported in several studies. In the present study, we have collected a large Chinese family harboring heteroplasmic mtDNA 1555A>G mutation with diverse clinical phenotypes. To investigate the relationship between the mutation load and the severity of hearing loss under Eastern Asian background, we performed clinical, molecular, genetic and phylogenic analysis. This pedigree was characterized by coexistence of eight subjects with homoplasmic mutation and ten subjects with various degrees of heteroplasmy, and the results suggested that there was a strong correlation between the mutation load and the severity/age-onset of hearing loss (r=0.758, p<0.001). We noticed that the mutation level of offspring was associated with their mothers' in this pedigree, which indicated that maybe exist a regular pattern during the process of the heteroplasmic transmission. In addition, analysis of the complete mtDNA genome of this family revealed that it belonged to Eastern Asian haplogroup B4C1. In addition, a rare homoplasmic mtDNA 9128T>C variant was identified, it located at a strictly conserved site of mtDNA ATP6 gene. 相似文献
45.
Zhang HT Zhan XB Zheng ZY Wu JR English N Yu XB Lin CC 《Applied microbiology and biotechnology》2012,93(1):367-379
A significant problem in scale-down cultures, rarely studied for metabolic characterization and curdlan-producing Agrobacterium sp. ATCC 31749, is the presence of dissolved oxygen (DO) gradients combined with pH control. Constant DO, between 5% and
75%, was maintained during batch fermentations by manipulating the agitation with PID system. Fermentation, metabolic and
kinetic characterization studies were conducted in a scale-down system. The curdlan yield, intracellular nucleotide levels
and glucose conversion efficiency into curdlan were significantly affected by DO concentrations. The optimum DO concentrations
for curdlan production were 45–60%. The average curdlan yield, curdlan productivity and glucose conversion efficiency into
curdlan were enhanced by 80%, 66% and 32%, respectively, compared to that at 15% DO. No apparent difference in the gel strength
of the resulting curdlan was detected. The comparison of curdlan biosynthesis and cellular nucleotide levels showed that curdlan
production had positive relationship with intracellular levels of UTP, ADP, AMP, NAD+, NADH and UDP-glucose. The curdlan productivity under 45% DO and 60% DO was different during 20–50 h. However, after 60 h
curdlan productivity of both conditions was similar. On that basis, a simple and reproducible two-stage DO control process
for curdlan production was developed. Curdlan production yield reached 42.8 g/l, an increase of 30% compared to that of the
single agitation speed control process. 相似文献
46.
根据对不同层次和专业医学生实验室生物安全教学的实践和体会,从教学指导思想、课程设置、考核评估等方面进行了总结,并分析了教学过程发现的问题,以期通过教学活动培养学生良好的实验室生物安全意识和素质。 相似文献
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Jian-Rong Wu Li-Jun Yu Xiao-Bei Zhan Zhi-Yong Zheng Jing Lu Chi-Chung Lin 《Process Biochemistry》2012,47(11):1552-1558
The mechanism of nitrogen signal regulating curdlan biosynthesis in Agrobacterium sp. ATCC 31749 was investigated. Under nitrogen limitation, more carbon flux is directed to curdlan synthesis with low specific growth rate. When ntrB and ntrC genes in Agrobacterium sp. were inactivated, NH4Cl utilization ability was significantly impaired in the ntrB and ntrC mutants and curdlan production was significantly reduced. Through proteomic analysis, nearly 40 proteins did not express in ntrC mutant compared with wild type strain. The levels of 22 proteins were significantly increased and 21 proteins were repressed after nitrogen exhaustion. Phosphoglucomutase activity in Agrobacterium sp. was also decreased. However, phosphoglucomutase activity in the ntrC mutant did not change. On that basis, an NtrC-dependent regulatory network for curdlan biosynthesis in response to nitrogen limitation in Agrobacterium sp. ATCC 31749 is proposed. 相似文献
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ISG15(Interferon stimulated gene 15,ISG15)蛋白是由干扰素诱导产生的一种泛素样蛋白分子,分子量大小约为15kD。ISG15同泛素分子相类似可以被共价结合于其他蛋白分子上,这种现象称为ISG化(ISGylation)现象。ISG化系统包括ISG15、UBE1L、UBCH8和HERC5四类蛋白分子,协同完成ISG化过程。ISG15及ISG化系统在抗病毒反应中具有重要作用。近几年对于ISG15的抗病毒作用和机制的研究已经有了很大的突破,ISG15的抗病毒作用也越来越受到人们重视,了解清楚ISG15抗病毒机制对于研制新的抗病毒药物及提出新的抗病毒策略具有重要意义。本文对ISG15在不同种病毒中的抗病毒机制研究进展进行了简要综述。 相似文献