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901.
In this study, a cell line, designated as Acipenser ruthenus testis (ART), was successfully established from testis tissues of the sterlet Acipenser ruthenus and characterized by studying and comparing the expression of specific genes between the cell line and the parent gonad tissues. The results suggested that the developed ART cell line was composed of a mixture of germ cells and somatic cells. Ploidy analysis indicated that the cell line exhibited a high degree of genetic stability and that the cells remained in a good proliferating state after being subcultured to passage 80. 相似文献
902.
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904.
He Youdi Chen Jun-Feng Yang Yan-Mei Huang Xiao-Hui Dong Xiao-Hui Yang Hui-Xin Cao Jun-Kai Jiang Xiao-Xia 《Molecular biology reports》2019,46(4):3991-3999
Molecular Biology Reports - Mesenchymal stem cells (MSCs) are self-renewing multipotent cells with immunoregulatory function, which makes them attractive candidates for regenerative medicine.... 相似文献
905.
Weiwei Dong Huiwu Tian Dengqiang Wang Lixiong Yu Xinbin Duan Shaoping Liu Daqing Chen 《Zeitschrift fur angewandte Ichthyologie》2019,35(6):1295-1299
Gobiobotia filifer is a small benthic fish distributed in Yangtze River Basin. The abundance of G. filifer increased after impoundment of Xiluodu Dam and Xiangjiaba Dam. The state of population structure and changes of genetic diversity before and after impoundment of Xiluodu Dam and Xiangjiaba Dam were interesting issues. However, efficient molecular markers were rare, which will limit us to solve above problems. Twenty‐eight expressed sequence tag SSRs (EST‐SSRs) were successfully identified and verified as stable amplification and polymorphic loci by polyacrylamide gel electrophoresis (PAGE) and capillary electrophoresis. The number of alleles at these EST‐SSR loci ranged from 3 to 14, the polymorphism information content values were 0.125–0.897, and the observed and expected heterozygosities were 0.0–0.857 and 0.132–0.928, respectively. Cross‐species amplification of the 28 loci developed in this study was examined in seven individuals of each of the 7 taxa. The amplification efficiency of 28 EST‐SSRs primer pairs is related to the distance of genetic relationship between cross‐species with G. filifer, and same subfamily species (Xenophysogobio boulengeri and Xenophysogobio nudicorpa) showed the highest (50%) amplification efficiency. These EST‐SSR markers could be used to analyse genetic diversity and population structure of G. filifer and related species. 相似文献
906.
The adsorption of the CO2/CH4 mixture in coal affects the CO2-enhanced coalbed methane recovery project. To gain a better understanding of CH4 and CO2 interaction with middle-rank coal, we developed a molecular concept with support for the sorption of CH4 and CO2 on Ximing-8 coal (XM-8) (1.8% vitrinite reflectance). A XM-8 coal model was built by using molecular dynamic (MD) simulations. The molecular simulations were established by the Grand Canonical Monte Carlo and MD methods to study the effects of the temperature, pressure, and species bulk mole fraction on the pure component adsorption isotherms, isosteric heat and adsorption selectivity. It turns out that the CO2 selectivity decreases as the pressure and its own bulk mole fraction increases, but it increases as temperature increases, and the selectivity values are not always greater than 1. The interactions between the small molecules and XM-8 were determined by using density functional theory. It was found that the interactions between the CO2 and XM-8 surface is greater, particularly for the heteroatoms than CH4. The adsorption selectivity and interaction were simultaneously used to reveal that the advantageously substituted range is high temperature, low pressure and a high content of heteroatoms. 相似文献
907.
Abnormal expression of tumour necrosis factor-α (TNF-α) can lead to various pathological reactions, such as arthritis, psoriasis, krone disease, etc. p38 mitogen-activated protein kinase (p38 MAPK) is an important signal transduction enzyme that plays important roles in influencing the release of intracellular TNF-α factor. It is very meaningful to study the targeting kinase with specific inhibitors in the treatment of related diseases. In order to achieve a deeper insight, it is necessary to analyse the structural characteristics and the action mode of the p38 MAPK inhibitors in the active site. In the study, a ligand-based common feature pharmacophore model and the receptor structure-based pharmacophore model were constructed, respectively. Their common chemical features consisted of the hydrophobic groups (H) and the hydrogen bond acceptors (A), and kept the consistency of spatial structure distribution. Then, the molecular docking and molecular dynamics simulation were performed with the eight training set compounds. The binding characteristics of molecules binding were described in the topological region of the active site. Finally, the structure–activity relationship (SAR) was obtained by analysing docking results with the different pharmacophore models. This research leads to the proposal of an interaction model in the p38 MAPK active site and provides guidance for the screening and design of more potent and selective p38 MAPK inhibitors. 相似文献
908.
Daniel Duran Xue Zeng Sheng Chih Jin Jungmin Choi Carol Nelson-Williams Bogdan Yatsula Jonathan Gaillard Charuta Gavankar Furey Qiongshi Lu Andrew T. Timberlake Weilai Dong Michelle A. Sorscher Erin Loring Jennifer Klein August Allocco Ava Hunt Sierra Conine Jason K. Karimy Kristopher T. Kahle 《Neuron》2019,101(3):429-443.e4
909.
Shiyue Cheng Zhi Li Junju He Shujun Fu Yumei Duan Qin Zhou Yuanliang Yan Xiaoyu Liu Liyu Liu Chang Feng Lu Zhang Jiang He Yuezhen Deng Lun-Quan Sun 《生物化学与生物物理学报:疾病的分子基础》2019,1865(6):1201-1213
Viral noncoding RNAs (Epstein–Barr virus-encoded RNAs, EBERs) are believed to play a critical role in the progression of lymphoma and nasopharyngeal carcinoma (NPC). However, the accurate mechanisms accounting for their oncogenic function have not been elucidated, especially in terms of interaction between tumor cells and mesenchymal cells. Here, we report that, in addition to NPC cells, EBERs are also found in endothelial cells in Epstein–Barr virus (EBV)-infected NPC parenchymal tissues, which implicates NPC-derived extracellular vesicles (EVs) in transmitting EBERs to endothelial cells. In support of this hypothesis, we first ascertained if EBERs could be transferred to endothelial cells via EVs isolated from NPC culture supernatant. Then, we clarified that EVs-derived EBERs could promote angiogenesis through stimulation of VCAM-1 expression. Finally, we explored the involvement of EBER recognition by TLR3 and RIG-I in NPC angiogenesis. Our observations collectively illustrate the significance and mechanism of EVs-derived EBERs in angiogenesis and underlie the interaction mechanisms between EBV-infected NPC cells and the tumor microenvironment. 相似文献
910.
Xianjun Sun Yubao Lv Junjun Wang HuiQin Cheng Jianhua Huang Yijie Du Jingcheng Dong 《Journal of cellular biochemistry》2019,120(10):18128-18141
Clinical application of oxaliplatin, a platinum-based chemotherapeutic agent, in cancer, especially colorectal cancer, is widely used. However, oxaliplatin-induced peripheral neurotoxicity (OIPN) has a high incidence, and to date, there have been few detailed studies on pathogenesis and treatment mechanisms. The present study was performed by using a proteomic approach to explore protein expression profiling of rats treated with oxaliplatin by multiplex isobaric tags for relative and absolute quantification labeling and two-dimensional liquid chromatography-tandem mass spectrometry. There were 74 proteins that showed different expression in sciatic nerve between control rats and OIPN model rats, with 53 upregulated proteins and 21 downregulated proteins detected in OIPN groups compared with control groups. On the basis of Gene Ontology clustering, these proteins were associated with biological processes (eg, muscle contraction, muscle system process, and skeletal muscle contraction), cellular component (eg, myofibril, contractile fiber, and contractile fiber part) and molecular function (structural constituent of muscle, hydro-lyase activity, and calcium ion binding). On the basis of Kyoto Encyclopedia of Genes and Genomes pathway database, these proteins were associated with African trypanosomiasis, malaria, nitrogen metabolism, etc. Real-time polymerase chain reaction, Western blot as well as immunohistochemistry analysis was performed to examine the expression of partially differential protein. In conclusion, our study establishes a protein expression profile of oxaliplatin-induced rats and mechanisms leading to OIPN development, and will be useful for developing novel diagnostic biomarkers and aiding in the prevention and control of OIPN. 相似文献