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11.
Formation of lymphocyte colonies under serum-free culture conditions in normal individuals and patients with chronic lymphocytic leukemia 总被引:1,自引:0,他引:1
Y C Dai G Z Hu H Y Niu Z Wen Z Fang X G Lu J Li Q Wu Y P Huang R Wen 《International journal of cell cloning》1987,5(6):480-491
This paper describes a culture system which supports the formation of B cell and some T cell colonies under serum-free conditions in peripheral blood samples of normal individuals and patients with chronic lymphocytic leukemia (CLL) of B cell type. In this system, serum is replaced by bovine serum albumin, transferrin, cholesterol, insulin and catalase or horseradish peroxidase. In addition, it is necessary to add staphylococcus protein A, mitomycin-treated T cells as feeders and phytohemagglutinin leukocyte-conditioned medium as a source of growth factors. The plating efficiency is greatly enhanced when normal cells are incubated with galactose oxidase prior to plating and when CLL cells are exposed sequentially to neuraminidase and galactose oxidase. 相似文献
12.
T Isobe Y I Fang D Muno T Okuyama D Ohmori F Yamakura 《Biochemistry international》1988,16(3):495-501
Alignment of the amino acid sequences of the Pseudomonas ovalis and Photobacterium leiognathi iron-superoxide dismutases (Fe-SODs) with the known sequences of the manganese-superoxide dismutases (Mn-SODs) shows that both types of SOD are highly homologous (33-53% identity) and share residues for the metal coordination. The amino acid residues that form the environment of the metal ions appear to be also conserved between the Fe- and Mn-SODs, except that the Phe-84 and Gln-154 in the Mn-SODs are replaced by Tyr and Ala, respectively, in the Fe-enzymes. Since this latter residue contributes to formation of the hydrophobic metal-ligand environment through hydrogen bonding with Trp-133 and Tyr-34 in the Mn-SODs, its substitution by Ala should cause different micro environments between the metal centers of the Fe- and Mn-SODs. This difference may account for the metal specificity of both types of SODs demonstrated by previous reconstitution experiments. 相似文献
13.
为探讨依兰花精油的香气质量,我们对云南省西双版纳产依兰花精油以及引种栽培于西双版纳的泰国种、老挝种的依兰花精油的化学成分在Finnigan-4510 GC/MS/DS上用毛细管柱进行了定性和定量分析,鉴定了44个化学成分。分析结果表明,依兰油的香气与品种及精油中的酯类、醇类和酚醚的含量有关。倍半萜烯类和倍半萜醇类的含量越高,其香气质量越差。就西双版纳产依兰油的香气而言,依兰花由青转黄时得到的精油远较绿花和花蕾油为好。 相似文献
14.
在甜椒(Capsicum annuum L.)中,靠近花粉中部的绒毡层自药隔产生,由较大的细胞组成,而花药外部区域的其余的绒毡层细胞较小,来自于初生壁层,前者的细胞具有大液泡和较大的细胞核,甲基绿-派罗宁和汞-溴酚蓝染色反应较后者弱,在造孢组织时期,二者液泡内都含有较大的球形的酸性磷酸酶颗粒,在以后的发育中,这种颗粒消失,在减数分裂时期,两种绒毡层的DNA,RNA和蛋白质合成活动增强,来自药隔的绒毡层积累了更多的DNA,绒毡层在解体时酸性磷本酶活性很高,两种不同的绒毡层退化过程相似,在全部发育过程中绒毡层内无淀粉粒。 相似文献
15.
鼻蝇亚科(Rhiniinae)是双翅目丽蝇科(Calliphoridae)中比较特殊的一大类群,因此也有人把它从丽蝇科分出,独立成科的。其特征有口上片突出如鼻状,后头上部大半是裸出的,约占整个头的宽度的范围内连粉被也缺如;r脉的上方沿后侧有一列明晰的小刚毛,翅下大结节上无立纤毛,下腋瓣裸。主要分布在东洋区、非洲区、大洋洲区以及古北区南缘。目前全世界已知有22个属,近300种,曾有报道其幼虫寄生于白蚁巢内及蝗 相似文献
16.
Kunio Takeda Atsuya Shigemura Satoshi Hamada Weicheng Gu Defu Fang Katsushi Sasa Kazuaki Hachiya 《Journal of Protein Chemistry》1992,11(2):187-192
The effect of protein conformations on the reaction rate of Ellman's reagent, 5,5-dithiobis (2-nitrobenzoic acid) (DTNB) with sulfhydryl (SH) groups of proteins was examined. The stopped-flow method was applied to follow the reaction of DTNB with SH group of two proteins, bovine serum albumin (BSA) and ovalbumin (OVA), at various concentrations of guanidine hydrochloride and urea. The rates for both the proteins were faster in guanidine than in urea. The rate sharply depended on the protein conformations, which were monitored by changes of helix contents on the basis of the circular dichroism measurements. The reaction rate of DTNB with SH groups of BSA was maximal around 2 M guanidine and 5 M urea. On the other hand, the reaction rate of DTNB with OVA was maximal at 3.5 M guanidine, while it gradually increased with an increase in the urea concentration. The amount of reactive SH group participating in the reaction with DTNB was also estimated by the absorbance change at 412 nm. The magnitudes of absorbance change for the reaction with free SH groups of OVA at low concentrations of the denaturants were appreciably smaller than those for BSA with one free SH group. Most of the four SH groups of OVA might react with DTNB above 5 M guanidine, although only a part of them did even at 9 M urea. 相似文献
17.
18.
19.
Evidence that the G1-S and G2-M transitions are controlled by different cdc2 proteins in higher eukaryotes. 总被引:71,自引:0,他引:71
Xenopus eggs contain two distinct cdc2 homologs of 34 and 32 kd. We show that the 32 kd cdc2 protein, like the 34 kd protein, is a kinase. However, unlike the 34 kd homolog, the 32 kd cdc2 kinase activity does not decrease dramatically at the end of mitosis. The 32 kd protein does not associate with mitotic cyclins B1 and B2 but does associate with cyclin A and a novel doublet of proteins of 54 kd that may regulate its activity. We also show that depletion of the 32 kd cdc2 homolog from a Xenopus extract blocks DNA replication, but does not inhibit entry into mitosis. By contrast, depletion of the 34 kd cdc2 homolog or absence of mitotic cyclins from an extract does not inhibit replication, but does block entry into mitosis. Our results indicate that in higher eukaryotes, DNA replication (G1-S) and mitosis (G2-M) may be controlled by distinctly different cdc2 proteins. 相似文献
20.
Rong-Xiang Fang Zhen Pang Dong-Ming Gao Ke-Qiang Mang Nam-Hai Chua 《Plant molecular biology》1991,17(6):1255-1257