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11.
Wang SF Si YX Wang ZJ Yin SJ Yang JM Qian GY 《International journal of biological macromolecules》2012,50(4):981-990
A folding study of creatine kinase from Pelodiscus sinensis has not yet been reported. To gain more insight into structural and folding mechanisms of P. sinensis CK (PSCK), denaturants such as SDS, guanidine HCl, and urea were applied in this study. We purified PSCK from the muscle of P. sinensis and conducted inhibition kinetics with structural unfolding studies under various conditions. The results revealed that PSCK was completely inactivated at 1.8 mM SDS, 1.05 M guanidine HCl, and 7.5 M urea. The kinetics via time-interval measurements showed that the inactivation by SDS, guanidine HCl, and urea were all first-order reactions with kinetic processes shifting from monophase to biphase at increasing concentrations. With respect to tertiary structural changes, PSCK was unfolded in different ways; SDS increased the hydrophobicity but retained the most tertiary structural conformation, while guanidine HCl and urea induced conspicuous changes in tertiary structures and initiated kinetic unfolding mechanisms. Our study provides information regarding PSCK and enhances our knowledge of the reptile-derived enzyme folding. 相似文献
12.
Mitigation of ammonia inhibition by internal dilution in high‐rate anaerobic digestion of food waste leachate and evidences of microbial community response 下载免费PDF全文
13.
Tao Tan Jun Wu Chenyang Si Shaoxing Dai Youyue Zhang Nianqin Sun E Zhang Honglian Shao Wei Si Pengpeng Yang Hong Wang Zhenzhen Chen Ran Zhu Yu Kang Reyna Hernandez-Benitez Llanos Martinez Martinez Estrella Nuñez Delicado W. Travis Berggren Juan Carlos Izpisua Belmonte 《Cell》2021,184(8):2020-2032.e14
14.
The production and secretion of streptokinase using OmpA signal sequence in E. coli was enhanced by removing the 13 N-terminal amino acids (SK(N13). The secretion level of SK(N13 protein into the extracellular medium was two times higher than that of wild-type streptokinase. About 4500 IU of SK(N13 protein per 1 ml LB-ampicillin medium was secreted into extracellular medium at 12 hours after induction. Fully active and enhanced extracellular preparation of the mutant streptokinase may be a potential alternative source for the simple downstream processing 相似文献
15.
We follow up on a suggestion by Rolls and co-workers, that the effects of competitive learning should be assessed on the shape
and number of spatial fields that dentate gyrus (DG) granule cells may form when receiving input from medial entorhinal cortex
(mEC) grid units. We consider a simple non-dynamical model where DG units are described by a threshold-linear transfer function,
and receive feedforward inputs from 1,000 mEC model grid units of various spacing, orientation and spatial phase. Feedforward
weights are updated according to a Hebbian rule as the virtual rodent follows a long simulated trajectory through a single
environment. Dentate activity is constrained to be very sparse. We find that indeed competitive Hebbian learning tends to
result in a few active DG units with a single place field each, rounded in shape and made larger by iterative weight changes.
These effects are more pronounced when produced with thousands of DG units and inputs per DG unit, which the realistic system
has available, than with fewer units and inputs, in which case several DG units persists with multiple fields. The emergence
of single-field units with learning is in contrast, however, to recent data indicating that most active DG units do have multiple
fields. We show how multiple irregularly arranged fields can be produced by the addition of non-space selective lateral entorhinal
cortex (lEC) units, which are modelled as simply providing an additional effective input specific to each DG unit. The mean
number of such multiple DG fields is enhanced, in particular, when lEC and mEC inputs have overall similar variance across
DG units. Finally, we show that in a restricted environment the mean size of the fields is unaltered, while their mean number
is scaled down with the area of the environment.
相似文献
Alessandro TrevesEmail: |
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中国南海侧扁软柳珊瑚中孕甾烷类化学成分的研究(英文) 总被引:1,自引:0,他引:1
从南海侧扁软柳珊瑚Subergorgia suberosa的二氯甲烷-甲醇提取物中首次分离鉴定了8个孕甾烷类化合物,经波谱鉴定为3β-O-palmitoyl-pregn-5-ene-20-one-3-ol (1),3β-O-palmitoyl-5α-pregn-20-one-3-ol (2),5α-pregn-1-ene-3,20-dione (3),3β,5α-pregn-20-one-3-ol (4),3β-pregn-5-ene-20-one-3-ol (5),3β,5β-pregn-20-one-3-ol (6),5β-pregn-3,20-dione (7),pregn-4-ene-3,20-dione (8).其中化合物1,2为新化合物. 相似文献
18.
Ali Riza Si?man Memduh Bülbül Canan Coker Banu Onvural 《Journal of trace elements in medicine and biology》2003,17(1):51-55
Cadmium is a nephrotoxic metal widely used in industry and the main source of Cd in general population is smoking. Considering that the source of Cd in cigarettes is the tobacco leaf, the exposure to Cd was evaluated in workers employed at a tobacco leaf processing factory. Blood and urinary Cd levels were measured by flameless atomic absorption spectrometry in 87 workers and 35 controls. Urinary enzymes, total protein, albumin and uric acid were also determined to investigate the possible nephrotoxic effects of Cd. Blood Cd levels were significantly higher in workers (1.63 +/- 1.95 microg/L) than in controls (0.91 +/- 1.15 microg/L) (p = 0.044). The increase observed in urinary Cd levels of workers was non significant (0.56 +/- 0.5 microg/g creatinine in workers and 0.46 +/- 0.5 microg/g creatinine in controls). Both in workers and in controls, subjects smoking >10 cigarettes/day showed significantly increased blood Cd levels compared to non-smokers (p = 0.000 and p = 0.011, respectively). In workers, urinary alkaline phosphatase (ALP), gamma glutamyl transferase (GGT), total protein, and uric acid were observed to be significantly increased (p = 0.013, p = 0.000, p = 0.000, p = 0.025, respectively), ALP, GGT and total protein being positively correlated with Cd in urine. In conclusion, the workers in the tobacco leaf processing factory were found to be exposed to Cd compared to the general population. The increase in the urinary enzymes and proteins suggests that an exposure to Cd affects kidney functions even below the toxic limits generally accepted. 相似文献
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Dong Chengya Wen Shaohong Zhao Shunying Sun Si Zhao Shangfeng Dong Wen Han Pingxin Chen Qingfang Gong Ting Chen Wentao Liu Wenqian Liu Xiangrong 《Neurochemical research》2021,46(4):755-769
Neurochemical Research - Cerebral ischemia leads to reactive astrogliosis and glial scar formation. Glial scarring can impede functional restoration during the recovery phase of stroke. Salidroside... 相似文献