广西北部湾地区生态足迹动态分析

生态足迹方法为定量计算地区可持续性发展提供了一种简单易行的方法。论文将在传统生态足迹方法的基础上结合目前国外最新生态足迹动态分析方法对广西北部湾地区从1998年到2005年的生态足迹进行了计算。结果表明:结合万元GDP占用生态足迹、生态足迹多样性指数和经济系统发展能力指数等指标的生态足迹动态分析方法,加强了传统生态足迹方法对地区可持续发展状况的反应。1998～2005年期间,广西北部湾地区,土地生产效率不断提高,经济发展能力在逐步增强,但是人均生态足迹呈逐年增加趋势,出现生态赤字,处于不可持续发展状态,仅林地资源与建筑用地资源还有少量人均盈余。     

Asymmetric reduction of prochiral ketones to chiral alcohols catalyzed by plants tissue

As an important organic compound, chiral alcohols are the key chiral building blocks to many single enantiomer pharmaceuticals. Asymmetric reduction of the corresponding prochiral ketones to produce the chiral alcohols by biocatalysis is one of the most promising routes. Asymmetric reduction of different kinds of non-natural prochiral ketones catalyzed by various plants tissue was studied in this work. Acetophenone, 4'-chloroacetophenone and ethyl 4-chloroacetoacetate were chosen as the model substrates for simple ketone, halogen-containing aromatic ketone and beta-ketoesters, respectively. Apple (Malus pumila), carrot (Daucus carota), cucumber (Cucumis sativus), onion (Allium cepa), potato (Soanum tuberosum), radish (Raphanus sativus) and sweet potato (Ipomoea batatas) were chosen as the biocatalysts. It was found that these kinds of prochiral ketoness could be reduced by these plants tissue with high enantioselectivity. Both R- and S-form configuration chiral alcohols could be obtained. The e.e. and chemical yield could reach about 98 and 80% respectively for acetophenone and 4'-chloroacetophenone reduction reaction with favorable plant tissue. And the e.e. and yield for ethyl 4-chloroacetoacetate reduction reaction was about 91 and 45% respectively.     

RETRACTED ARTICLE: ShRNA-mediated gene silencing of Heat shock protein 70 inhibits human colon cancer cell growth in vitro and in vivo

Molecular and Cellular Biochemistry -     

Effects of Melatonin on Growth,Physiology and Gene Expression in Rice Seedlings Under Cadmium Stress

Melatonin (MLT) is a hormonal substance found in many organisms and can improve plant stress resistance. In this study, the japonica rice variety Y32 and indica rice variety NJ6 were cultivated in hydroponics under different concentrations of CdCl₂ at the two-leaf stage. The growth, physiological and biochemical responses of the seedlings and the expression of cadmium (Cd)-related genes under exogenous melatonin (MLT) treatment were assessed. The results indicated that Cd stress destroyed the dynamic balance between reactive oxygen species (ROS) production and removal, resulting in ROS accumulation, membrane lipid peroxidation, and impaired growth and development. Following the application of exogenous MLT to rice seedlings, increases in plant biomass including both underground and above-ground areas were observed. MLT also scavenged the inhibition of superoxide dismutase (SOD) and peroxidase (POD) in a concentration dependent manner in response to Cd stress. Catalase (CAT) activity and malondialdehyde (MDA) expression also decreased following MLT treatment. Amongst the six Cd-related genes assessed, five genes were down-regulated and one was up-regulated in response to MLT treatment. Taken together, these data demonstrate that MLT improves the resilience of rice seedlings at the biochemical, physiological, and molecular levels, and diminishes the damage caused by Cd stress.     

The configuration,solvatochromism and metallo‐responses of two novel cyano‐containing oligo(phenylene‐vinylene) derivatives

Two novel cyano‐containing oligo(phenylenevinylene) (OPV) derivatives have been designed and synthesized. Photophysical and sensing properties of the two compounds were studied. Such studies reveal the intramolecular charge transfer process between cyano groups and OPV core. The results showed that the alkyl difference of substituted OPV leads to the changes of molecular configuration and metallo‐response of two compounds. Copyright © 2010 John Wiley & Sons, Ltd.     

新疆艾比湖沉积物中免培养放线菌的多样性北大核心CSCD

【目的】采用免培养的方法研究新疆艾比湖湖底沉积物放线菌组成及多样性。【方法】采集并混合5份艾比湖湖底沉积物样本,并提取其总DNA,采用放线菌通用引物对其16S r RNA基因序列进行Touchdown PCR,构建放线菌16S r RNA基因文库。蓝白斑筛选后随机挑选白色克隆分析,利用限制性内切酶HhaⅠ进行酶切分型,挑选具有独特限制性片段差异的阳性克隆进行测序分析。序列经Chimera Check检测,BLAST同源比对及构建16S r RNA基因序列系统发育树。【结果】随机挑选192个白色克隆,其中166个为阳性克隆,选取51个具有独特限制性片段差异的克隆进行序列分析。测序结果进行比对以及Chimera Check检测后,共获得36个可操作单元(Operational Taxonomic Units,OTUs),Gen Bank注册号为KR182090-KR182131。文库覆盖度结果表明克隆文库涵盖了本环境中90.4%的放线菌类群。聚类结果显示,艾比湖湖底沉积物中放线菌分为2个类群,第1个类群属于放线菌门(Actinobacteria),放线菌纲(Actinobacteria)中的放线菌目(Actinomycetales)、丙酸杆菌目(Propionibacteriales)、微球菌目(Micrococcales)和棒杆菌目(Corynebacteriales)4个目,该类群占克隆文库18.1%;另外1个类群属于Unclassified Actinobacteria的类群,分为3个不同的group,占整个克隆文库的81.9%。【结论】新疆艾比湖湖底沉积物中存在多种未知的放线菌类群。     

Inhibition of heme oxygenase-1 enhances the chemosensitivity of laryngeal squamous cell cancer Hep-2 cells to cisplatin

It has been previously reported that cisplatin is a well-known anticancer drug being used against a wide range of malignancies including head and neck, ovarian and non-small cell lung carcinoma, and demonstrated its anticancer activity by reacting with DNA or changing cell structure, immune response, reactive oxygen species level (ROS). In this research we proved that cisplatin induced cell injuries and heme oxygenase-1 (HO-1) expression in laryngeal squamous cell cancer Hep-2 cells through ROS generation. The induction of HO-1 clearly protected Hep-2 cells from cisplatin-induced cell death and ROS reaction, and the inhibitor of HO-1 enhanced the cell death and ROS generation induced by cisplatin. Furthermore, the HO-1 expression induced by cisplatin was strongly inhibited by the knockdown of nuclear factor-erythroid-2-related factor-2 (Nrf-2), and the oxidative damages induced by cisplatin were significantly enhanced. Therefore, it may be concluded that the inhibition of HO-1 or the knockdown of Nrf-2 significantly enhanced cisplatin’s anticancer effects on Hep-2 cells. In clinic, with the overexpression of HO-1 in laryngeal squamous cancer tissues, the combination of cisplatin with the inhibitor of HO-1 or Nrf-2 siRNA may act as a new method to the treatment of laryngeal squamous cancer.     

Anti-inflammatory isoflavones and isoflavanones from the roots of Pongamia pinnata (L.) Pierre

A phytochemical study on the roots of Pongamia pinnata afforded five new isoflavone and isoflavanone derivatives (1–5), including two previously undescribed phenylisoflavones possessing an 1,2-oxetane ring, along with 21 known compounds (6–26) among which compound 18 is the first time to be isolated from nature. The structures of the isolated compounds were determined on the basis of 1D, 2D NMR, and HRESIMS. The absolute configurations of the compounds were assigned via analysis of the specific rotations and circular dichroism (CD) spectra, as well as by comparison of the calculated and experimental electronic circular dichroism (ECD) data. All the isolated compounds were evaluated for their inhibitory effects on NO production in LPS-stimulated BV-2 microglial cells. Twelve compounds exhibited different levels of inhibitory effects against NO production, and compound 1 showed the best activity with an IC₅₀ value at 9.0?μM.