云南羯布罗香树脂的化学成分

从云南产羯布罗香(Dipterocarpus tubinatus Gaertn. f.)树脂中分离得到6个三萜化合物,鉴定为羟基达玛烯酮—Ⅱ(hydroxydammarenone-Ⅱ,达玛烯二醇—Ⅱ(dammarenediol—Ⅱ),白桦脂酸(betulonk acid),亚细亚酸(asiatic acid),3,23-O-异丙叉亚细亚酸(3,23-O-isopropylidene asiatic acid)和崩大碗酸(madasiatic acid)。其中崩大碗酸系首次从龙脑香科树脂中分离得到。     

国产五种菊属植物的核型研究

本文对国产5种菊属植物的核型进行了研究,结果为:D. indicum 2n=2x=18=16m 2st,2n=4x=36=28m 6sm 2st;D. lavandulifolium 2n=2x=18=14m 4sm,2n=4x=36=28m 6sm 2st;D. lavandulifolium var.seticuspe 2n=2x=18=12m 6sm;D. chanetii 2n=4x=36=20m 14sm 2st,2n=6x=54=38m 14sm 2st;D. potentilloides2n=2x=18=14m 2sm 2st;D. vestitum 2n=6x=54=38m 16sm.核型分析的结果表明,本文所研究类群中出现的多倍体多为异源多倍体;根据对核型资料、形态特征及地理分布特点的综合分析,作者认为多倍化是菊属野生种进行的主要途径。     

室旁核在剌激猫肾神经传入纤维引起的血压效应中的应用

The purpose of this study is to investigate the role of paraventricular nucleus of the hypothalamus (PVN) and alpha 1 adrenergic receptor of PVN in the pressor responses to stimulation of renal afferent nerve in alpha 1-chloralose-anesthetized cats with carotid sinoaortic denervation and vagotomy. The pressor response to stimulation of renal afferent nerve consisted of a primary and a second components. The primary component response was completely blocked while the second component was not blocked by autonomic blocking agents (hexomethonium and atropine). Bilateral lesions of PVN greatly attenuated the pressor response before and after autonomic blockade. Intracerebroventricular and PVN injection alpha 1, adrenergic antagonist (prazosin) significantly decreased in the pressor response to stimulation of renal afferent nerve. These results indicate that paraventricular nucleus of the hypothalamus and alpha 1 adrenergic receptors in central nervous system, especially in PVN, play an important role in the pressor responses to stimulation of renal afferent nerve.     

Na~+、K~+离子通道阻滞剂对离体大鼠黄体细胞孕酮生成的影响

本工作用二种离子通道阻断剂四乙胺(TEA)和河豚毒素(TTX)来研究 Na~+、K~+通道的改变对大鼠黄体细胞孕酮生成的影响。10~(-3)mol/L 的 TEA 或 TTX 均使孕酮分泌量显著增加,而这种促进效应可被酪氨酸(Tyr)完全阻断。Tyr 对 TEA 或 TTX 与 hCG 联合所引起的孕酮分泌也有抑制作用。上述实验说明跨黄体细胞内外的 K~+和 Na~+浓度差与孕酮分泌有关。     

刺激中缝背核压抑大鼠小脑浦肯野细胞对苔状和爬行纤维传入的反应

本文在麻醉并制动的大鼠上观察了中缝背核(DR)条件刺激对由苔状和爬行纤维传入引起的小脑浦肯野细胞(PC)诱发反应的影响。主要结果有:(1)刺激大脑皮层感觉运动区可以引起苔状和爬行纤维向对侧小脑皮层第Ⅵ和Ⅶ小叶的传入,因而在该小叶上记录到 PC 的诱发简单锋电位(SS)和复杂锋电位(CS)反应,潜伏期分别是8—25和12—30ms。(2)以不影响PC 自发 SS 和 CS 活动的阈下强度刺激 DR,可显著地压抑 PC 对于刺激感觉运动皮层引起的苔状和爬行纤维兴奋所产生的诱发 SS 和 CS 反应,这种压抑作用可持续数百毫秒。(3)DR条件刺激对 PC 的诱发 SS 和 CS 反应的压抑作用可以被静脉注射5-HT 受体阻断剂羟甲丙基甲基麦角酰胺所减弱或阻断。上述结果表明 DR 的5-HT 能纤维传入可以降低苔状和爬行纤维对 PC 的突触作用效力,抑或降低 PC 对突触传入的反应敏感性,提示中缝-小脑5-HT,能纤维传入系统参与了小脑某些重要的神经活动过程。     

蟾蜍血液淋巴细胞姐妹染色单体互换及细胞周期动力学研究

本文对中华大蟾蜍Bufo bufo gargarizans血液淋巴细胞在植物血球凝集素(PHA)刺激条件下的细胞周期动力学及丝裂霉素C(MMC)诱发姐妹染色单体互换(SCE)的敏感性进行了研究。用姐妹染色单体差别染色方法确定细胞的分裂次数。实验结果表明,中华大蟾蜍血液淋巴细胞在体外培养2天后出现第二次分裂细胞,3天后达峰值(占分裂细胞中期相的38.9％),此后,其百分比逐日下降,培养后4天出现第三次分裂细胞,第5天出现第四次分裂细胞。Brdu浓度在8—24μg/ml范围内对SCE本底无影响。6ng/ml MMC所诱发的SCEs比对照组高3倍以上,说明中华大蟾蜍对MMC的诱变作用相当敏感。     

血友病患者血清中淋巴腺病病毒/人T细胞Ⅲ型病毒抗体检测

对浙江省1982～1984年注射了美国产浓缩Ⅶ因子制剂的18例血友病患者,用酶联免疫吸附法(ELTSA)检测了血清中淋巴腺病病毒/人T细胞Ⅲ型病毒(LAV/HTLV-Ⅲ)抗体,发现4例阳性,并经免疫荧光试验和Western印迹法证实。2例应用了国产浓缩Ⅷ因子者抗体阴性。一例从美国来华旅游死于艾滋病者,LAV/HTLV-Ⅲ抗体阳性。本研究证明,LAV/HTLVⅢ病毒巳通过美国生产的Ⅷ因子制剂传入中国。     

HBsAg合成肽段的免疫源性和抗肽血清与天然HBsAg的结合测定

我们将三种乙型肝炎表面抗原的合成肽段在联结载体或不接载体的情况下,分别制备成4种免疫原对兔子进行了免疫。大部分兔子都产生了抗肽抗体。其中抗P_(122-48)(auw)与抗P_(122-148)(adw)都能与天然HBsAg反应,而抗P_(122-148)(adw)的抗HBs活力比文献报道的都高,通过结构分析表明:含有天然蛋白上免疫显性区域的免疫原是合成疫苗的理想选者。     

毛丝鼠体温调节发育的研究

毛丝鼠幼仔,六日龄已基本上达到恒温水平。吮乳期幼仔的静止代谢率较威体高;二日龄前,代谢率变化不符合体表面积定律;二日龄后,趋向成年恒温动物代谢类型,而符合体表面积定律。幼仔在25℃环境的热能消耗比20℃时少。     

Loading and translocation of assimilate in the fine veins of sunflower leaves

Abstract The time course of loading and transport of assimilate in sunflower leaves was examined by pulse labelling with ¹⁴CO₂, followed by freeze drying or freeze substitution, and dry autoradiography at both low and high resolution. The five classes of veins, V1-V5 (V5 being smallest), show a division of function: V5 and V4 are engaged in loading and short distance transport; V3 to V1, in long distance translocation. The first high concentration of ¹⁴C is found in two or three phloem parenchyma cells (intermediary cells) of V5 and V4 veins. The sieve elements of V5 and V4 veins do not show comparable concentrations of ¹⁴C at any time. Recently assimilated ¹⁴C is transported by the intermediary cells for distances of about 0.5 mm to the V3 veins. In V3 to V1 veins translocation is in the sieve tubes. Transport in V5 and V4 veins is in two directions, that in V3 to V1, in one direction towards the petiole. The high concentration of ¹⁴C formed in the intermediary cells does not increase further as the assimilate moves to the sieve tubes of the V3 veins, and so is probably the origin of the gradient that drives translocation.