全文获取类型
收费全文 | 8840篇 |
免费 | 665篇 |
国内免费 | 654篇 |
出版年
2024年 | 19篇 |
2023年 | 114篇 |
2022年 | 282篇 |
2021年 | 492篇 |
2020年 | 293篇 |
2019年 | 371篇 |
2018年 | 377篇 |
2017年 | 300篇 |
2016年 | 367篇 |
2015年 | 567篇 |
2014年 | 683篇 |
2013年 | 701篇 |
2012年 | 828篇 |
2011年 | 717篇 |
2010年 | 430篇 |
2009年 | 401篇 |
2008年 | 461篇 |
2007年 | 377篇 |
2006年 | 292篇 |
2005年 | 247篇 |
2004年 | 192篇 |
2003年 | 186篇 |
2002年 | 162篇 |
2001年 | 143篇 |
2000年 | 128篇 |
1999年 | 106篇 |
1998年 | 71篇 |
1997年 | 83篇 |
1996年 | 82篇 |
1995年 | 60篇 |
1994年 | 47篇 |
1993年 | 45篇 |
1992年 | 63篇 |
1991年 | 49篇 |
1990年 | 49篇 |
1989年 | 33篇 |
1988年 | 39篇 |
1987年 | 26篇 |
1986年 | 23篇 |
1985年 | 36篇 |
1984年 | 33篇 |
1983年 | 14篇 |
1982年 | 15篇 |
1981年 | 8篇 |
1978年 | 11篇 |
1974年 | 11篇 |
1973年 | 7篇 |
1971年 | 9篇 |
1967年 | 7篇 |
1966年 | 9篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
911.
Van Camp JA Hu LY Kostlan C Lefker B Li J Mitchell L Wang Z Yue WS Carroll M Dettling D Du D Pocalyko D Wade K 《Bioorganic & medicinal chemistry letters》2007,17(20):5529-5532
A series of substituted 4-aryl-2-trifluoromethylbenzonitrile analogs were evaluated in the human androgen receptor binding and cellular functional assays. Analogs with sufficient in vitro binding and cellular potency (IC(50)<200 nM) were tested in the progesterone receptor binding assay for selectivity and in the Golden Syrian hamster ear model for in vivo efficacy. Within the series, compound 4 e was identified to be the most active analog in vivo (wax ester inhibition=86%). 相似文献
912.
Single nucleotide polymorphisms in chicken <Emphasis Type="Italic">lmbr1</Emphasis> gene were associated with chicken growth and carcass traits
下载免费PDF全文
![点击此处可从《中国科学C辑(英文版)》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Huang YanQun Du XiaoHui Deng XueMei Qiu XiangPin Wang ChunKao Chen Wen Li Ning Wu ChangXin 《中国科学C辑(英文版)》2007,50(1):62-69
Lmbr1 is the key candidate gene controlling vertebrate limb development, but its effects on animal growth and carcass traits have
never been reported. In this experiment, lmbr1 was taken as the candidate gene affecting chicken growth and carcass traits. T/C and G/A mutations located in exon 16 and
one A/C mutation located in intron 5 of chicken lmbr1 were detected from Silky, White Plymouth Rock broilers and their F2 crossing chickens by PCR-SSCP and sequencing methods. The analysis of variance (ANOVA) results suggests that T/C polymorphism
of exon 16 had significant association with eviscerated yield rate (EYR), gizzard rate (GR), shank and claw rate (SCR) and
shank girth (SG); A/C polymorphism of intron 5 was significantly associated with SCR, liver rate and head-neck weight (HNW),
while both sites had no significant association with other growth and carcass traits. These results demonstrate that lmbr1 gene could be a genetic locus or linked to a major gene significantly affecting these growth and carcass traits in chicken. 相似文献
913.
黄酮类化合物抗肿瘤研究进展 总被引:6,自引:0,他引:6
黄酮类化合物是广泛存在于自然界中的一类多酚化合物,有许多潜在的药用价值,抗肿瘤活性是其研究热点之一。其抗肿瘤作用主要表现在抑制细胞增殖、诱导细胞凋亡、干预信号转导、影响细胞周期、影响血管生成、克服肿瘤细胞多药耐药性等方面。文中就黄酮类化合物抗肿瘤作用的研究进展进行综述。 相似文献
914.
Redistribution of carbon flux in Torulopsis glabrata by altering vitamin and calcium level 总被引:1,自引:0,他引:1
Manipulation of cofactor (thiamine, biotin and Ca(2+)) levels as a potential tool to redistribute carbon flux was studied in Torulopsis glabrata. With sub-optimization of vitamin in fermentation medium, the carbon flux was blocked at the key node of pyruvate, and 69 g/L pyruvate was accumulated. Increasing the concentrations of thiamine and biotin could selectively open the valve of carbon flux from pyruvate to pyruvate dehydrogenase complex, the pyruvate carboxylase (PC) pathway and the channel into the TCA cycle, leading to the over-production of alpha-ketoglutarate. In addition, the activity of PC was enhanced with Ca(2+) present in fermentation medium. By combining high concentration's vitamins and CaCO(3) as the pH buffer, a batch culture was conducted in a 7-L fermentor, with the pyruvate concentration decreased to 21.8 g/L while alpha-ketoglutarate concentration increased to 43.7 g/L. Our study indicated that the metabolic flux could be redistributed to overproduce desired metabolites with manipulating the cofactor levels. Furthermore, the manipulation of vitamin level provided an alternative tool to realize metabolic engineering goals. 相似文献
915.
Du L Chen J Qi Y Li D Yuan C Lin MC Yew DT Kung HF Yu JC Lai L 《International journal of nanomedicine》2007,2(4):805-812
We report the preparation of a non-polymer coated superparamagnetic nanoparticle that is stable and biocompatible both in vitro and in vivo. The non-polymer, betaine, is a natural methylating agent in mammalian liver with active surface property. Upon systemic administration, the nanoparticle has preferential biodistribution in mammalian liver and exhibits good reduction of relaxivity time and negative enhancement for the detection of hepatoma nodules in rats using MRI. Our data demonstrate that the non-polymer coated superparamagnetic nanoparticle should have potential applications in biomedicine. 相似文献
916.
Kim YS Xiao HZ Du EQ Cai GS Lu SY Qi YP 《Journal of biochemistry and molecular biology》2007,40(4):571-576
Three anti-apoptosis genes, Ls-iap2, iap3 and p49 were found in Leucania separata multiple nuclear polyhedrovirus. Amino acid sequence homology of Ls-IAP2 and Ls-IAP3 with Op-IAP2 and Op-IAP3 from Orgyia pseddotsugata MNPV were 20% and 42%, while that of Ls-P49 is 28% with Sl-P49 from Spodoptera littorolis MNPV. Ls-IAP2 contains one baculoviral IAP repeat (BIR) domain followed by a RING domain, while Ls-IAP3 contains two BIRs and a RING. Ls-P49 contains a reactive site loop, predicted cleavage site (KKLD(74) downward arrow G) that is different from Sl-P49 (TVID(94) downward arrow G). Expressed Ls-iap3 or Ls-p49 under presence of actinomycin D in SF9 cells, DNA ladder assay revealed that Ls- IAP3 or Ls-P49 could block the apoptosis of SF9 cells induced by actinomycin D. Replication of p35 deficient-mutant Autographa californica MNPV in SF9 cells was also rescued when Ls-iap3 or Ls-p49 was expressed transiently. No anti-apoptotic activity was observed for Ls-IAP2. The results showed that both of Ls-IAP3 and Ls-P49 were functional apoptotic suppressors in SF9 cells. 相似文献
917.
The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield. 相似文献
918.
Helicobacter pylori activates extracellular-signal related (ERK) kinases in gastric epithelial cells, via transactivation of the EGF receptor (EGFR). H. pylori activation of EGFR may be relevant to epithelial hyperproliferation and gastric carcinogenesis. The aim of this study was to develop an 'In-Cell Western' (ICW) assay for quantitative examination of H. pylori-induced epithelial signalling, to enable the role of the EGFR in H. pylori-induced phosphorylation of ERK in epithelial cells to be ascertained. H. pylori strains were co-incubated with A431 and AGS cells. pERK and total ERK were quantified in situ using ICW analysis. H. pylori strains both with, and without a cag PAI, and Helicobacter felis, significantly increased pERK levels in A431 cells. The EGFR inhibitor EKB-569 dose-dependently reduced H. pylori-induced ERK phosphorylation in A431 and AGS cells. A significantly lower reduction was observed with cag+ strains in A431 but not AGS cells. The cag PAI was not necessary for EGFR signal transactivation. These data suggest that H. pylori induces pERK in epithelial cells partly via the EGFR pathway. Additional signalling mechanisms are likely to be involved in H. pylori-induced ERK phosphorylation. ICW analysis is a rapid quantitative method for evaluating the effects of inhibitors on H. pylori-induced cell signalling pathways of relevance to gastric carcinogenesis. 相似文献
919.
920.
Li ZS Qiao RP Du Q Yang ZJ Zhang LR Zhang PZ Liang ZC Zhang LH 《Bioconjugate chemistry》2007,18(4):1017-1024
A novel class of aminoisonucleoside was synthesized and incorporated into a luciferase gene-targeting siRNA. Structural and functional analyses of such a kind of siRNAs indicated that sense strand modifications with aminoisonucleoside at the 3' or 5' terminal, such as ssIso-1 and ssIso-2, have less effect on RNA duplex thermal and serum stabilities, and their functional activities are also comparable to their native siRNAs. In contrast, antisense strand modifications with aminoisonucleoside at the corresponding positions, such as asIso-2 or asIso-1, bring a striking negative effect on RNA duplex stability but still maintain around 40-50% of gene knockdown. 相似文献